Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a ubiquitous pathogen responsible for several severe infections. This study aimed to investigate the adhesive properties and antibiotic resistance among clinical <i>S. aureus</i> isolated from Hail Hospital Provin...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1353 |
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| author | Khulood Fahad Alabbosh Tarek Zmantar Abdulrahman S. Bazaid Mejdi Snoussi Emira Noumi |
| author_facet | Khulood Fahad Alabbosh Tarek Zmantar Abdulrahman S. Bazaid Mejdi Snoussi Emira Noumi |
| author_sort | Khulood Fahad Alabbosh |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a ubiquitous pathogen responsible for several severe infections. This study aimed to investigate the adhesive properties and antibiotic resistance among clinical <i>S. aureus</i> isolated from Hail Hospital Province, Kingdom of Saudi Arabia (KSA), using molecular approaches. This study was conducted according to the ethical committee at Hail’s guidelines on twenty-four <i>S. aureus</i> isolates. A polymerase chain reaction (PCR) was performed to identify genes encoding the β-lactamase resistance (<i>bla</i>Z), methicillin resistance (<i>mec</i>A), fluoroquinolone resistance (<i>nor</i>A), nitric oxide reductase (<i>nor</i>B), fibronectin (<i>fnb</i>A and <i>fnb</i>B), clumping factor (<i>clf</i>A) and intracellular adhesion factors (<i>ica</i>A and <i>ica</i>D). This qualitative study tested adhesion based on exopolysaccharide production on Congo red agar (CRA) medium and biofilm formation on polystyrene by <i>S. aureus</i> strains. Among 24 isolates, the <i>cna</i> and blaz were the most prevalent (70.8%), followed by <i>nor</i>B (54.1%), <i>clf</i>A (50.0%), <i>nor</i>A (41.6%), <i>mec</i>A and <i>fnb</i>B (37.5%) and <i>fnb</i>A (33.3%). The presence of <i>ica</i>A/<i>ica</i>D genes was demonstrated in almost all tested strains in comparison to the reference strain, <i>S. aureus</i> ATCC 43300. The phenotypic study of adhesion showed that all tested strains had moderate biofilm-forming capacity on polystyrene and represented different morphotypes on a CRA medium. Five strains among the twenty-four harbored the four genes of resistance to antibiotics (<i>mec</i>A, <i>nor</i>A, <i>nor</i>B and <i>bla</i>z). Considering the genes of adhesion (<i>cna</i>, <i>clf</i>A, <i>fnb</i>A and <i>fnb</i>B), these genes were present in 25% of the tested isolates. Regarding the adhesive properties, the clinical isolates of <i>S. aureus</i> formed biofilm on polystyrene, and only one strain (S17) produced exopolysaccharides on Congo red agar. All these results contribute to an understanding that the pathogenesis of clinical <i>S. aureus</i> isolates is due to their antibiotic resistance and adhesion to medical material. |
| first_indexed | 2024-03-11T03:28:09Z |
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| last_indexed | 2024-03-11T03:28:09Z |
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| spelling | doaj.art-2f54099cd28946d8981fbd5f74e794042023-11-18T02:35:32ZengMDPI AGMicroorganisms2076-26072023-05-01115135310.3390/microorganisms11051353Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound InfectionsKhulood Fahad Alabbosh0Tarek Zmantar1Abdulrahman S. Bazaid2Mejdi Snoussi3Emira Noumi4Department of Biology, College of Science, University of Hail, Hail 2440, Saudi ArabiaLaboratory of Analysis, Treatment, Valorization of Environmental, and Product Pollutants, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, TunisiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Hail, Hail 55476, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, Hail 2440, Saudi Arabia<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a ubiquitous pathogen responsible for several severe infections. This study aimed to investigate the adhesive properties and antibiotic resistance among clinical <i>S. aureus</i> isolated from Hail Hospital Province, Kingdom of Saudi Arabia (KSA), using molecular approaches. This study was conducted according to the ethical committee at Hail’s guidelines on twenty-four <i>S. aureus</i> isolates. A polymerase chain reaction (PCR) was performed to identify genes encoding the β-lactamase resistance (<i>bla</i>Z), methicillin resistance (<i>mec</i>A), fluoroquinolone resistance (<i>nor</i>A), nitric oxide reductase (<i>nor</i>B), fibronectin (<i>fnb</i>A and <i>fnb</i>B), clumping factor (<i>clf</i>A) and intracellular adhesion factors (<i>ica</i>A and <i>ica</i>D). This qualitative study tested adhesion based on exopolysaccharide production on Congo red agar (CRA) medium and biofilm formation on polystyrene by <i>S. aureus</i> strains. Among 24 isolates, the <i>cna</i> and blaz were the most prevalent (70.8%), followed by <i>nor</i>B (54.1%), <i>clf</i>A (50.0%), <i>nor</i>A (41.6%), <i>mec</i>A and <i>fnb</i>B (37.5%) and <i>fnb</i>A (33.3%). The presence of <i>ica</i>A/<i>ica</i>D genes was demonstrated in almost all tested strains in comparison to the reference strain, <i>S. aureus</i> ATCC 43300. The phenotypic study of adhesion showed that all tested strains had moderate biofilm-forming capacity on polystyrene and represented different morphotypes on a CRA medium. Five strains among the twenty-four harbored the four genes of resistance to antibiotics (<i>mec</i>A, <i>nor</i>A, <i>nor</i>B and <i>bla</i>z). Considering the genes of adhesion (<i>cna</i>, <i>clf</i>A, <i>fnb</i>A and <i>fnb</i>B), these genes were present in 25% of the tested isolates. Regarding the adhesive properties, the clinical isolates of <i>S. aureus</i> formed biofilm on polystyrene, and only one strain (S17) produced exopolysaccharides on Congo red agar. All these results contribute to an understanding that the pathogenesis of clinical <i>S. aureus</i> isolates is due to their antibiotic resistance and adhesion to medical material.https://www.mdpi.com/2076-2607/11/5/1353<i>Staphylococcus aureus</i>antibiotic resistanceadhesion genesbiofilmmolecular approach |
| spellingShingle | Khulood Fahad Alabbosh Tarek Zmantar Abdulrahman S. Bazaid Mejdi Snoussi Emira Noumi Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections Microorganisms <i>Staphylococcus aureus</i> antibiotic resistance adhesion genes biofilm molecular approach |
| title | Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections |
| title_full | Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections |
| title_fullStr | Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections |
| title_full_unstemmed | Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections |
| title_short | Antibiotics Resistance and Adhesive Properties of Clinical <i>Staphylococcus aureus</i> Isolated from Wound Infections |
| title_sort | antibiotics resistance and adhesive properties of clinical i staphylococcus aureus i isolated from wound infections |
| topic | <i>Staphylococcus aureus</i> antibiotic resistance adhesion genes biofilm molecular approach |
| url | https://www.mdpi.com/2076-2607/11/5/1353 |
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