Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring
Summary: Background and aims: We aimed to evaluate the impact of perinatal food manipulation on skeletal characteristics and insulin levels of Wistar rats at the age of 1 year. Methods: Sixty-seven first-time pregnant rats were randomized, to either normally fed (Control Diet, CD), food-restricted...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Clinical Nutrition Open Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667268520300048 |
| _version_ | 1818329410565046272 |
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| author | Nikolaos Syggelos Areti Augoulea Eleni Armeni George Kaparos Homeira Vafaei Ismene Dontas Katerina Marinou George Vaggos Panagiota Raptou Irene Lambrinoudaki Makarios Eleftheriades Kypros H. Nicolaides |
| author_facet | Nikolaos Syggelos Areti Augoulea Eleni Armeni George Kaparos Homeira Vafaei Ismene Dontas Katerina Marinou George Vaggos Panagiota Raptou Irene Lambrinoudaki Makarios Eleftheriades Kypros H. Nicolaides |
| author_sort | Nikolaos Syggelos |
| collection | DOAJ |
| description | Summary: Background and aims: We aimed to evaluate the impact of perinatal food manipulation on skeletal characteristics and insulin levels of Wistar rats at the age of 1 year. Methods: Sixty-seven first-time pregnant rats were randomized, to either normally fed (Control Diet, CD), food-restricted (FR), or fat-fed (FF), from the 12th gestational day, and gave birth on the 21st day of pregnancy. Pups born to FR-mothers were divided into: fetal growth restricted (FGR) and non-FGR, based on their birth weight. Maternal food manipulation continued through the lactation period. Following delivery, all neonates were cross-fostered until the 25th day postpartum; the offspring of normally-fed mothers were lactated by FR-, FF- or CD-fed mothers. A similar process was followed for the offspring of mothers FF- or FR-during pregnancy. On the 26th day postpartum, all pups were weaned to the diet of their foster mother until one year old. Bone density was assessed by peripheral quantitative computed tomography. Results: FF/FF rats had lower values of total bone density and total/subcortical area compared to CD/CD. FF/FR showed lower subcortical density compared to FF/FF group. FGR/CD showed lower values of all assessed skeletal parameters compared to those receiving CD throughout the experiment. Non-FGR/FF rats had higher values of all assessed skeletal parameters compared to those food restricted postnatally. FGR-pups that were fat-fed postnatally had higher insulin vs rats FF/FR. Similar insulin levels were identified in rats fat-fed postnatally, irrespective of prenatal food-restriction or high-fat diet. Conclusions: Perinatal food manipulation is associated with distinct skeletal acquisition and insulin levels’ profiles in Wistar rats at the first year of life. |
| first_indexed | 2024-12-13T12:47:37Z |
| format | Article |
| id | doaj.art-2f5903b5776f42a4852d6630769f4e5a |
| institution | Directory Open Access Journal |
| issn | 2667-2685 |
| language | English |
| last_indexed | 2024-12-13T12:47:37Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Nutrition Open Science |
| spelling | doaj.art-2f5903b5776f42a4852d6630769f4e5a2022-12-21T23:45:26ZengElsevierClinical Nutrition Open Science2667-26852021-02-01353447Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspringNikolaos Syggelos0Areti Augoulea1Eleni Armeni2George Kaparos3Homeira Vafaei4Ismene Dontas5Katerina Marinou6George Vaggos7Panagiota Raptou8Irene Lambrinoudaki9Makarios Eleftheriades10Kypros H. Nicolaides11Second Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, GreeceSecond Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, GreeceSecond Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, GreeceBiochemical Laboratory, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, GreeceDepartment of Obstetrics and Gynecology, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, IranLaboratory for Research of the Musculoskeletal System, Th. Garofalidis, National and Kapodistrian University of Athens, KAT Hospital, Kifissia, Attica, GreeceGreek Ministry of Rural Development and Food, Department of Animal Welfare for Farm and Laboratory Animals, Athens, GreeceDepartment of Obstetrics and Gynecology, General Hospital of Arta, Arta, GreeceLaboratory for Research of the Musculoskeletal System, Th. Garofalidis, National and Kapodistrian University of Athens, KAT Hospital, Kifissia, Attica, GreeceSecond Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, Greece; Corresponding author. 27, Themistokleous street, Dionysos GR-14578, Athens, Greece. Tel.: +30 210 6410944; fax: +30 210 6410325.Second Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Aretaieio Hospital, Athens, Greece; Harris Birthright Research Centre for Fetal Medicine, King's College Hospital, London, UKHarris Birthright Research Centre for Fetal Medicine, King's College Hospital, London, UKSummary: Background and aims: We aimed to evaluate the impact of perinatal food manipulation on skeletal characteristics and insulin levels of Wistar rats at the age of 1 year. Methods: Sixty-seven first-time pregnant rats were randomized, to either normally fed (Control Diet, CD), food-restricted (FR), or fat-fed (FF), from the 12th gestational day, and gave birth on the 21st day of pregnancy. Pups born to FR-mothers were divided into: fetal growth restricted (FGR) and non-FGR, based on their birth weight. Maternal food manipulation continued through the lactation period. Following delivery, all neonates were cross-fostered until the 25th day postpartum; the offspring of normally-fed mothers were lactated by FR-, FF- or CD-fed mothers. A similar process was followed for the offspring of mothers FF- or FR-during pregnancy. On the 26th day postpartum, all pups were weaned to the diet of their foster mother until one year old. Bone density was assessed by peripheral quantitative computed tomography. Results: FF/FF rats had lower values of total bone density and total/subcortical area compared to CD/CD. FF/FR showed lower subcortical density compared to FF/FF group. FGR/CD showed lower values of all assessed skeletal parameters compared to those receiving CD throughout the experiment. Non-FGR/FF rats had higher values of all assessed skeletal parameters compared to those food restricted postnatally. FGR-pups that were fat-fed postnatally had higher insulin vs rats FF/FR. Similar insulin levels were identified in rats fat-fed postnatally, irrespective of prenatal food-restriction or high-fat diet. Conclusions: Perinatal food manipulation is associated with distinct skeletal acquisition and insulin levels’ profiles in Wistar rats at the first year of life.http://www.sciencedirect.com/science/article/pii/S2667268520300048Perinatal nutritionPeripheral quantitative computed tomographyWistar rats |
| spellingShingle | Nikolaos Syggelos Areti Augoulea Eleni Armeni George Kaparos Homeira Vafaei Ismene Dontas Katerina Marinou George Vaggos Panagiota Raptou Irene Lambrinoudaki Makarios Eleftheriades Kypros H. Nicolaides Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring Clinical Nutrition Open Science Perinatal nutrition Peripheral quantitative computed tomography Wistar rats |
| title | Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring |
| title_full | Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring |
| title_fullStr | Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring |
| title_full_unstemmed | Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring |
| title_short | Impact of prenatal and postnatal nutritional manipulation on bone quality in adult Wistar rats offspring |
| title_sort | impact of prenatal and postnatal nutritional manipulation on bone quality in adult wistar rats offspring |
| topic | Perinatal nutrition Peripheral quantitative computed tomography Wistar rats |
| url | http://www.sciencedirect.com/science/article/pii/S2667268520300048 |
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