Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis
A lahar-laden soil is described as marginal soil due to its poor physicochemical and biological properties and is hypothesised to have low biodiversity of soil microbes that would help support plant growth. Thus, the study aimed to assess the presence of soil microorganisms in lahar-laden soils in S...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Acta Agriculturae Scandinavica. Section B, Soil and Plant Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09064710.2022.2163281 |
| _version_ | 1797684320106708992 |
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| author | Israel M. Guanzon Maria Luisa T. Mason Purisima P. Juico Fernan T. Fiegalan |
| author_facet | Israel M. Guanzon Maria Luisa T. Mason Purisima P. Juico Fernan T. Fiegalan |
| author_sort | Israel M. Guanzon |
| collection | DOAJ |
| description | A lahar-laden soil is described as marginal soil due to its poor physicochemical and biological properties and is hypothesised to have low biodiversity of soil microbes that would help support plant growth. Thus, the study aimed to assess the presence of soil microorganisms in lahar-laden soils in Sta. Rita, Pampanga, with their ability as beneficial microbes for crop production. Vigna radiata was used as the trap crop and grown for a maximum of 35 days in a microcosm experiment where all variables were similar except for the location of the sampling site. Through the sequence analysis of the 16S rRNA gene, five representative isolates revealed close resemblance to the genus Bradyrhizobium and Rhizobium, while the others were classified as non-rhizobial endophytes, namely, Pseudomonas, Agrobacterium, and Leifsonia. These results indicate that the lahar-laden soils in the sampling site harbour some agronomically-important genera of microorganisms. Surprisingly, this is the first time that the species Leifsonia xyli was identified and isolated in acidic lahar-laden soil. In contrast, it is hypothesised that the presence of L.xyli in this study might be attributed to the acidic soil pH, leading to the suppression of rhizobia in root nodules. |
| first_indexed | 2024-03-12T00:27:57Z |
| format | Article |
| id | doaj.art-2f613d875d90428695688851b510183c |
| institution | Directory Open Access Journal |
| issn | 0906-4710 1651-1913 |
| language | English |
| last_indexed | 2024-03-12T00:27:57Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Acta Agriculturae Scandinavica. Section B, Soil and Plant Science |
| spelling | doaj.art-2f613d875d90428695688851b510183c2023-09-15T10:26:28ZengTaylor & Francis GroupActa Agriculturae Scandinavica. Section B, Soil and Plant Science0906-47101651-19132023-12-0173111210.1080/09064710.2022.21632812163281Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysisIsrael M. Guanzon0Maria Luisa T. Mason1Purisima P. Juico2Fernan T. Fiegalan3Pampanga State Agricultural UniversityCentral Luzon State UniversityCentral Luzon State UniversityCentral Luzon State UniversityA lahar-laden soil is described as marginal soil due to its poor physicochemical and biological properties and is hypothesised to have low biodiversity of soil microbes that would help support plant growth. Thus, the study aimed to assess the presence of soil microorganisms in lahar-laden soils in Sta. Rita, Pampanga, with their ability as beneficial microbes for crop production. Vigna radiata was used as the trap crop and grown for a maximum of 35 days in a microcosm experiment where all variables were similar except for the location of the sampling site. Through the sequence analysis of the 16S rRNA gene, five representative isolates revealed close resemblance to the genus Bradyrhizobium and Rhizobium, while the others were classified as non-rhizobial endophytes, namely, Pseudomonas, Agrobacterium, and Leifsonia. These results indicate that the lahar-laden soils in the sampling site harbour some agronomically-important genera of microorganisms. Surprisingly, this is the first time that the species Leifsonia xyli was identified and isolated in acidic lahar-laden soil. In contrast, it is hypothesised that the presence of L.xyli in this study might be attributed to the acidic soil pH, leading to the suppression of rhizobia in root nodules.http://dx.doi.org/10.1080/09064710.2022.2163281acid soilbradyrhizobiumleifsonia xylilife on landvigna radiata |
| spellingShingle | Israel M. Guanzon Maria Luisa T. Mason Purisima P. Juico Fernan T. Fiegalan Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis Acta Agriculturae Scandinavica. Section B, Soil and Plant Science acid soil bradyrhizobium leifsonia xyli life on land vigna radiata |
| title | Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis |
| title_full | Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis |
| title_fullStr | Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis |
| title_full_unstemmed | Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis |
| title_short | Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis |
| title_sort | isolation of three genera of microorganisms in lahar laden soils of sta rita pampanga philippines through the 16s rrna gene sequence analysis |
| topic | acid soil bradyrhizobium leifsonia xyli life on land vigna radiata |
| url | http://dx.doi.org/10.1080/09064710.2022.2163281 |
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