Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum
The aim of this study was to measure the activity of enzymes which reflect cardiotoxic action in rats of novel synthetic 4-thiazolidone derivatives – 3882, 3288 and 3833 that demonstrated antineoplastic effect in vitro towards 60 lines of human tumor cells tested in the framework of the program of s...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-12-01
|
| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/07/Kobylinska_6_14.pdf |
| _version_ | 1797428294330613760 |
|---|---|
| author | L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva, N. E. Mitina О. S. Zаichenko B. S. Zіmenkovsky R. S. Stoika |
| author_facet | L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva, N. E. Mitina О. S. Zаichenko B. S. Zіmenkovsky R. S. Stoika |
| author_sort | L. I. Kоbylinska |
| collection | DOAJ |
| description | The aim of this study was to measure the activity of enzymes which reflect cardiotoxic action in rats of novel synthetic 4-thiazolidone derivatives – 3882, 3288 and 3833 that demonstrated antineoplastic effect in vitro towards 60 lines of human tumor cells tested in the framework of the program of screening new anticancer drugs at the National Cancer Institute (USA). Such action of these compounds was compared with the effect of well known anticancer agent doxorubicin and after conjugation of all above mentioned substances with new polyethylenglycol-containing polymeric comb-like carrier that was synthesized by the authors. Among the biochemical indicators of cardiotoxic action of anticancer agents, activity of the following enzymes in rat blood serum showed to be the most informative: creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase. Tenfold injection of doxorubicin in a dose of 5.5 mg/kg of weight caused rats’ death, while 3882, 3288 and 3833 preparations had not such action. Application of the doxorubicin in combination with polymeric carrier prolonged the survival time to 20 days. Thus, the injection of anticancer agents in a complex with polymeric carrier provides a significant decrease in their cardiotoxicity that was confirmed by the corresponding changes in the activity of marker enzymes: creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase in blood serum of treated rats. |
| first_indexed | 2024-03-09T08:55:56Z |
| format | Article |
| id | doaj.art-2f623843b15247488d5d50844481ca02 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:55:56Z |
| publishDate | 2014-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-2f623843b15247488d5d50844481ca022023-12-02T12:56:56ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032014-12-01866849510.15407/ubj86.06.084Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serumL. I. Kоbylinska0 D. Ya. Havrylyuk1 А. О. Ryabtseva,2 N. E. Mitina3О. S. Zаichenko4 B. S. Zіmenkovsky5 R. S. Stoika6Danylo Halytsky Lviv National Medical University, Ukraine;Danylo Halytsky Lviv National Medical University, Ukraine;Lviv National Polytechnic University, UkraineLviv National Polytechnic University, UkraineLviv National Polytechnic University, UkraineDanylo Halytsky Lviv National Medical University, Ukraine;Іnstitute of Cell Biology, National Academy of Sciences of Ukraine, LvivThe aim of this study was to measure the activity of enzymes which reflect cardiotoxic action in rats of novel synthetic 4-thiazolidone derivatives – 3882, 3288 and 3833 that demonstrated antineoplastic effect in vitro towards 60 lines of human tumor cells tested in the framework of the program of screening new anticancer drugs at the National Cancer Institute (USA). Such action of these compounds was compared with the effect of well known anticancer agent doxorubicin and after conjugation of all above mentioned substances with new polyethylenglycol-containing polymeric comb-like carrier that was synthesized by the authors. Among the biochemical indicators of cardiotoxic action of anticancer agents, activity of the following enzymes in rat blood serum showed to be the most informative: creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase. Tenfold injection of doxorubicin in a dose of 5.5 mg/kg of weight caused rats’ death, while 3882, 3288 and 3833 preparations had not such action. Application of the doxorubicin in combination with polymeric carrier prolonged the survival time to 20 days. Thus, the injection of anticancer agents in a complex with polymeric carrier provides a significant decrease in their cardiotoxicity that was confirmed by the corresponding changes in the activity of marker enzymes: creatine kinase, lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase in blood serum of treated rats.http://ukrbiochemjournal.org/wp-content/uploads/2015/07/Kobylinska_6_14.pdf4-thiazolidone derivativescardiotoxicitydoxorubicinenzymatic activitypolyethylenglycol-containing polymeric comb-like carrierбpolyethylenglycol-containing polymeric comb-like carrier |
| spellingShingle | L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva, N. E. Mitina О. S. Zаichenko B. S. Zіmenkovsky R. S. Stoika Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum The Ukrainian Biochemical Journal 4-thiazolidone derivatives cardiotoxicity doxorubicin enzymatic activity polyethylenglycol-containing polymeric comb-like carrierб polyethylenglycol-containing polymeric comb-like carrier |
| title | Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum |
| title_full | Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum |
| title_fullStr | Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum |
| title_full_unstemmed | Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum |
| title_short | Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol-containing polymeric carrier in the rat blood serum |
| title_sort | study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4 thiazolidinone derivatives and doxorubicin in complexes with polyethylenglycol containing polymeric carrier in the rat blood serum |
| topic | 4-thiazolidone derivatives cardiotoxicity doxorubicin enzymatic activity polyethylenglycol-containing polymeric comb-like carrierб polyethylenglycol-containing polymeric comb-like carrier |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/07/Kobylinska_6_14.pdf |
| work_keys_str_mv | AT likobylinska studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT dyahavrylyuk studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT aoryabtseva studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT nemitina studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT oszaichenko studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT bszímenkovsky studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum AT rsstoika studyofratbloodserumbiochemicalindicatorsofcardiotoxicactionofnovelantitumor4thiazolidinonederivativesanddoxorubicinincomplexeswithpolyethylenglycolcontainingpolymericcarrierintheratbloodserum |