The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma
Background: Non-Hodgkin lymphomas (NHLs) are a diverse group of mature lymphoid neoplasms with a wide range of cellular and histological presentations. Numerous environmental and genetic factors affect the incidence of these neoplasms; however the exact causes are beyond the current knowledge. ...
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| Format: | Article |
| Language: | English |
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College of medicine/ University of Diyala
2019-06-01
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| Series: | Diyala Journal of Medicine |
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| Online Access: | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/272 |
| _version_ | 1797586297144999936 |
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| author | Mayasa Abdulla Ali Hassan M Naif Qasim Sharhan Al-Mayah |
| author_facet | Mayasa Abdulla Ali Hassan M Naif Qasim Sharhan Al-Mayah |
| author_sort | Mayasa Abdulla Ali |
| collection | DOAJ |
| description |
Background: Non-Hodgkin lymphomas (NHLs) are a diverse group of mature lymphoid neoplasms with a wide range of cellular and histological presentations. Numerous environmental and genetic factors affect the incidence of these neoplasms; however the exact causes are beyond the current knowledge.
Objective: to investigate the association of genetic polymorphisms in two single nucleotide polymorphisms, which are IL-10-1082A/G and IL-12p401188A/ C, with the incidence NHLs among Iraqi patients.
Materials and Methods: Whole blood samples were collected from 55 confirmed patients with NHLs and from 40 family-unrelated, age-matched apparently healthy individuals to represent the control group. DNA was extracted from blood samples and allele specific polymerase chain reaction (AS-PCR) technique was used for genotyping of the two SNPs using specific primers.
Results: The SNP IL-10-1082A/G appeared in three genotypes: AA, AG and GG which represented 36.36%, 45.45% and 18.18%% respectively in NHL patients compared to 32.5%, 35% and 32.5%% respectively with insignificant neither between genotypes nor alleles. Similarly, the SNP IL-12p401188A/C had three genotypes which were AA, AC and CC. These genotypes represented 32.73%, 38.18% and 29.09% respectively among NHL patients and 52.5%, 37.5% and 10% respectively among controls with significant difference for the homozygous mutant genotype (OR=4.667, 95%CI=1.319-16.512, P=0.017).
Conclusion: IL-10-1082A/G polymorphism has no effect on the incidence of NHLs, while allele C of the SNP IL-12p401188A/C could be considered as a risk factor for these neoplasms.
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| first_indexed | 2024-03-11T00:21:20Z |
| format | Article |
| id | doaj.art-2f63f4081ba24ec3971d0a073fd568e4 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-11T00:21:20Z |
| publishDate | 2019-06-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-2f63f4081ba24ec3971d0a073fd568e42023-11-18T23:17:36ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822019-06-01101The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin LymphomaMayasa Abdulla AliHassan M NaifQasim Sharhan Al-Mayah Background: Non-Hodgkin lymphomas (NHLs) are a diverse group of mature lymphoid neoplasms with a wide range of cellular and histological presentations. Numerous environmental and genetic factors affect the incidence of these neoplasms; however the exact causes are beyond the current knowledge. Objective: to investigate the association of genetic polymorphisms in two single nucleotide polymorphisms, which are IL-10-1082A/G and IL-12p401188A/ C, with the incidence NHLs among Iraqi patients. Materials and Methods: Whole blood samples were collected from 55 confirmed patients with NHLs and from 40 family-unrelated, age-matched apparently healthy individuals to represent the control group. DNA was extracted from blood samples and allele specific polymerase chain reaction (AS-PCR) technique was used for genotyping of the two SNPs using specific primers. Results: The SNP IL-10-1082A/G appeared in three genotypes: AA, AG and GG which represented 36.36%, 45.45% and 18.18%% respectively in NHL patients compared to 32.5%, 35% and 32.5%% respectively with insignificant neither between genotypes nor alleles. Similarly, the SNP IL-12p401188A/C had three genotypes which were AA, AC and CC. These genotypes represented 32.73%, 38.18% and 29.09% respectively among NHL patients and 52.5%, 37.5% and 10% respectively among controls with significant difference for the homozygous mutant genotype (OR=4.667, 95%CI=1.319-16.512, P=0.017). Conclusion: IL-10-1082A/G polymorphism has no effect on the incidence of NHLs, while allele C of the SNP IL-12p401188A/C could be considered as a risk factor for these neoplasms. https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/272Non-Hodgkin’s lymphoma, single nucleotide polymorphism, IL-10-1082A/G, IL-12p40 1188A/C. |
| spellingShingle | Mayasa Abdulla Ali Hassan M Naif Qasim Sharhan Al-Mayah The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma Diyala Journal of Medicine Non-Hodgkin’s lymphoma, single nucleotide polymorphism, IL-10-1082A/G, IL-12p40 1188A/C. |
| title | The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma |
| title_full | The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma |
| title_fullStr | The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma |
| title_full_unstemmed | The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma |
| title_short | The Impact of Genetic Variants in IL-10 and IL-12p40 on the Susceptibility to Non Hodgkin Lymphoma |
| title_sort | impact of genetic variants in il 10 and il 12p40 on the susceptibility to non hodgkin lymphoma |
| topic | Non-Hodgkin’s lymphoma, single nucleotide polymorphism, IL-10-1082A/G, IL-12p40 1188A/C. |
| url | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/272 |
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