miR-21-5p protects IL-1β-induced human chondrocytes from degradation
Abstract Objective Osteoarthritis (OA) is a prevalent degenerative disease caused by various factors. MicroRNAs are important regulators in OA. MiR-21-5p expression is decreased in OA cartilage, but the effects of modulating miR-21-5p on cartilage regeneration are unknown. Therefore, our aim was to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-019-1160-7 |
| _version_ | 1828116083503005696 |
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| author | Hai Zhu Xin Yan Meng Zhang Feng Ji Shouguo Wang |
| author_facet | Hai Zhu Xin Yan Meng Zhang Feng Ji Shouguo Wang |
| author_sort | Hai Zhu |
| collection | DOAJ |
| description | Abstract Objective Osteoarthritis (OA) is a prevalent degenerative disease caused by various factors. MicroRNAs are important regulators in OA. MiR-21-5p expression is decreased in OA cartilage, but the effects of modulating miR-21-5p on cartilage regeneration are unknown. Therefore, our aim was to investigate the effects of miR-21-5p on cartilage metabolism of OA chondrocytes. Design We used IL-1β (10 ng/ml) to mimic OA chondrocytes. OA chondrocytes were transfected with miR-21-5p, the gene expression of COL2A1, MMP13, and ADAMTS5 was detected by qPCR. At the same time, COL2A1, MMP13, and ADAMTS5 were analyzed at the protein level by Western blot. CCK8 measured the cell’s viability and SA-β-gal detected the cell’s senescence. Results Upregulation of miR-21-5p had increased COL2A1 expression and decreased MM P13 and ADAMTS5 expression, which were in accord with Western blot data. SA-β-gal activity significantly increased, the viability was decreased in OA chondrocytes, and upregulation of miR-21-5p can decrease the SA-β-gal activity and increase cell viability. Conclusion MiR-21-5p might be a potential disease-modifying compound in OA, as it promotes hyaline cartilage production. These results provided that novel insights into the important function in OA pathological development. |
| first_indexed | 2024-04-11T12:51:33Z |
| format | Article |
| id | doaj.art-2f6551836abd4255bd0acd7eab6b1cd3 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T12:51:33Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-2f6551836abd4255bd0acd7eab6b1cd32022-12-22T04:23:12ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2019-05-011411910.1186/s13018-019-1160-7miR-21-5p protects IL-1β-induced human chondrocytes from degradationHai Zhu0Xin Yan1Meng Zhang2Feng Ji3Shouguo Wang4Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Orthopaedics, Affiliated Hospital of Nantong UniversityDepartment of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityAbstract Objective Osteoarthritis (OA) is a prevalent degenerative disease caused by various factors. MicroRNAs are important regulators in OA. MiR-21-5p expression is decreased in OA cartilage, but the effects of modulating miR-21-5p on cartilage regeneration are unknown. Therefore, our aim was to investigate the effects of miR-21-5p on cartilage metabolism of OA chondrocytes. Design We used IL-1β (10 ng/ml) to mimic OA chondrocytes. OA chondrocytes were transfected with miR-21-5p, the gene expression of COL2A1, MMP13, and ADAMTS5 was detected by qPCR. At the same time, COL2A1, MMP13, and ADAMTS5 were analyzed at the protein level by Western blot. CCK8 measured the cell’s viability and SA-β-gal detected the cell’s senescence. Results Upregulation of miR-21-5p had increased COL2A1 expression and decreased MM P13 and ADAMTS5 expression, which were in accord with Western blot data. SA-β-gal activity significantly increased, the viability was decreased in OA chondrocytes, and upregulation of miR-21-5p can decrease the SA-β-gal activity and increase cell viability. Conclusion MiR-21-5p might be a potential disease-modifying compound in OA, as it promotes hyaline cartilage production. These results provided that novel insights into the important function in OA pathological development.http://link.springer.com/article/10.1186/s13018-019-1160-7OsteoarthritisChondrocytesmiR-21-5pCOL2A1ADAMTS5MMP13 |
| spellingShingle | Hai Zhu Xin Yan Meng Zhang Feng Ji Shouguo Wang miR-21-5p protects IL-1β-induced human chondrocytes from degradation Journal of Orthopaedic Surgery and Research Osteoarthritis Chondrocytes miR-21-5p COL2A1 ADAMTS5 MMP13 |
| title | miR-21-5p protects IL-1β-induced human chondrocytes from degradation |
| title_full | miR-21-5p protects IL-1β-induced human chondrocytes from degradation |
| title_fullStr | miR-21-5p protects IL-1β-induced human chondrocytes from degradation |
| title_full_unstemmed | miR-21-5p protects IL-1β-induced human chondrocytes from degradation |
| title_short | miR-21-5p protects IL-1β-induced human chondrocytes from degradation |
| title_sort | mir 21 5p protects il 1β induced human chondrocytes from degradation |
| topic | Osteoarthritis Chondrocytes miR-21-5p COL2A1 ADAMTS5 MMP13 |
| url | http://link.springer.com/article/10.1186/s13018-019-1160-7 |
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