Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch
<i>Aphis craccivora</i> Koch is a polyphagous and major pest of leguminous crops causing significant damage by reducing the yield. Repeated application of synthetic insecticides for the control of aphids has led to development of resistance. Therefore, the present study aimed to screen t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/3/633 |
| _version_ | 1827659843487399936 |
|---|---|
| author | Surekha Kumari Shudh Kirti Dolma Anmol Upendra Sharma S. G. Eswara Reddy |
| author_facet | Surekha Kumari Shudh Kirti Dolma Anmol Upendra Sharma S. G. Eswara Reddy |
| author_sort | Surekha Kumari |
| collection | DOAJ |
| description | <i>Aphis craccivora</i> Koch is a polyphagous and major pest of leguminous crops causing significant damage by reducing the yield. Repeated application of synthetic insecticides for the control of aphids has led to development of resistance. Therefore, the present study aimed to screen the insecticidal activity of root/stem extracts/fractions, and pure molecules from <i>Cissampelos pareira</i> Linnaeus against <i>A. craccivora</i> for identification of lead(s). Among root extract/fractions, the <i>n</i>-hexane fraction was found most effective (LC<sub>50</sub> = 1828.19 mg/L) against <i>A. craccivora</i>, followed by parent extract (LC<sub>50</sub> = 2211.54 mg/L). Among stem extract/fractions, the <i>n</i>-hexane fraction (LC<sub>50</sub> = 1246.92 mg/L) was more effective than the water and <i>n</i>-butanol fractions. Based on GC and GC-MS analysis, among different compounds identified in the <i>n</i>-hexane fraction of root and stem, ethyl palmitate (known to possess insecticidal activity) was present in the highest concentration (24.94 to 52.95%) in both the fractions. Among pure molecules, pareirarineformate was found most effective (LC<sub>50</sub> = 1491.93 mg/L) against <i>A. craccivora</i>, followed by cissamine (LC<sub>50</sub> = 1556.31 mg/L). Parent extract and fractions of <i>C. pareira</i> possess promising activity against aphid. Further, field bio-efficacy studies are necessary to validate the current findings for the development of botanical formulation. |
| first_indexed | 2024-03-09T23:29:59Z |
| format | Article |
| id | doaj.art-2f67f4ce65ac49748c105f9e086b2fca |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:29:59Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2f67f4ce65ac49748c105f9e086b2fca2023-11-23T17:09:55ZengMDPI AGMolecules1420-30492022-01-0127363310.3390/molecules27030633Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> KochSurekha Kumari0Shudh Kirti Dolma1Anmol2Upendra Sharma3S. G. Eswara Reddy4Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, IndiaAcademy of Scientific and Innovative Research (AcSIR), CSIR-HRDC Campus, Ghaziabad 201002, Uttar Pradesh, IndiaChemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, IndiaChemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, IndiaAcademy of Scientific and Innovative Research (AcSIR), CSIR-HRDC Campus, Ghaziabad 201002, Uttar Pradesh, India<i>Aphis craccivora</i> Koch is a polyphagous and major pest of leguminous crops causing significant damage by reducing the yield. Repeated application of synthetic insecticides for the control of aphids has led to development of resistance. Therefore, the present study aimed to screen the insecticidal activity of root/stem extracts/fractions, and pure molecules from <i>Cissampelos pareira</i> Linnaeus against <i>A. craccivora</i> for identification of lead(s). Among root extract/fractions, the <i>n</i>-hexane fraction was found most effective (LC<sub>50</sub> = 1828.19 mg/L) against <i>A. craccivora</i>, followed by parent extract (LC<sub>50</sub> = 2211.54 mg/L). Among stem extract/fractions, the <i>n</i>-hexane fraction (LC<sub>50</sub> = 1246.92 mg/L) was more effective than the water and <i>n</i>-butanol fractions. Based on GC and GC-MS analysis, among different compounds identified in the <i>n</i>-hexane fraction of root and stem, ethyl palmitate (known to possess insecticidal activity) was present in the highest concentration (24.94 to 52.95%) in both the fractions. Among pure molecules, pareirarineformate was found most effective (LC<sub>50</sub> = 1491.93 mg/L) against <i>A. craccivora</i>, followed by cissamine (LC<sub>50</sub> = 1556.31 mg/L). Parent extract and fractions of <i>C. pareira</i> possess promising activity against aphid. Further, field bio-efficacy studies are necessary to validate the current findings for the development of botanical formulation.https://www.mdpi.com/1420-3049/27/3/633<i>Cissampelos pareira</i>isoquinoline alkaloidsbioassayaphid |
| spellingShingle | Surekha Kumari Shudh Kirti Dolma Anmol Upendra Sharma S. G. Eswara Reddy Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch Molecules <i>Cissampelos pareira</i> isoquinoline alkaloids bioassay aphid |
| title | Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch |
| title_full | Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch |
| title_fullStr | Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch |
| title_full_unstemmed | Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch |
| title_short | Insecticidal Activity of Extracts, Fractions, and Pure Molecules of <i>Cissampelos pareira</i> Linnaeus against Aphid, <i>Aphis craccivora</i> Koch |
| title_sort | insecticidal activity of extracts fractions and pure molecules of i cissampelos pareira i linnaeus against aphid i aphis craccivora i koch |
| topic | <i>Cissampelos pareira</i> isoquinoline alkaloids bioassay aphid |
| url | https://www.mdpi.com/1420-3049/27/3/633 |
| work_keys_str_mv | AT surekhakumari insecticidalactivityofextractsfractionsandpuremoleculesoficissampelospareirailinnaeusagainstaphidiaphiscraccivoraikoch AT shudhkirtidolma insecticidalactivityofextractsfractionsandpuremoleculesoficissampelospareirailinnaeusagainstaphidiaphiscraccivoraikoch AT anmol insecticidalactivityofextractsfractionsandpuremoleculesoficissampelospareirailinnaeusagainstaphidiaphiscraccivoraikoch AT upendrasharma insecticidalactivityofextractsfractionsandpuremoleculesoficissampelospareirailinnaeusagainstaphidiaphiscraccivoraikoch AT sgeswarareddy insecticidalactivityofextractsfractionsandpuremoleculesoficissampelospareirailinnaeusagainstaphidiaphiscraccivoraikoch |