Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots
Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042926/full |
| _version_ | 1811240638431100928 |
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| author | Jian Ju Jian Ju Xinyu Zhang Lin Li Lin Li Sagar Regmi Guoqiang Yang Guoqiang Yang Guoqiang Yang Shixing Tang Shixing Tang |
| author_facet | Jian Ju Jian Ju Xinyu Zhang Lin Li Lin Li Sagar Regmi Guoqiang Yang Guoqiang Yang Guoqiang Yang Shixing Tang Shixing Tang |
| author_sort | Jian Ju |
| collection | DOAJ |
| description | Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform for the detection of SARS-CoV-2-specific IgM and IgG by the aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein (SSP). The aggregation-induced emission carbon dots (AIE-CDs) are one of the best prospect fluorescent probe materials for exhibiting high emission efficiency in both aggregate and solid states. The AIE-CDs were synthesized and displayed dual fluorescence emission, which provides a new perspective for the design of a high sensitivity testing system. In this work, the novel LFA platform adopted the AIE carbon dots, which are used to detect SARS-CoV-2-specific IgM and IgG conveniently. Furthermore, this sensor had a low LOD of 100 pg/ml. Therefore, this newly developed strategy has potential applications in the areas of public health for the advancement of clinical research. |
| first_indexed | 2024-04-12T13:23:45Z |
| format | Article |
| id | doaj.art-2f683412fe914b7c893854ec52347fa3 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T13:23:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2f683412fe914b7c893854ec52347fa32022-12-22T03:31:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-10-011010.3389/fbioe.2022.10429261042926Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dotsJian Ju0Jian Ju1Xinyu Zhang2Lin Li3Lin Li4Sagar Regmi5Guoqiang Yang6Guoqiang Yang7Guoqiang Yang8Shixing Tang9Shixing Tang10Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaOujiang Lab, Wenzhou, ChinaWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaSchool of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, ChinaDepartment of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH, United StatesWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaSchool of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Photochemistry, Institute of Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaDepartment of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, ChinaUnderstanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform for the detection of SARS-CoV-2-specific IgM and IgG by the aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein (SSP). The aggregation-induced emission carbon dots (AIE-CDs) are one of the best prospect fluorescent probe materials for exhibiting high emission efficiency in both aggregate and solid states. The AIE-CDs were synthesized and displayed dual fluorescence emission, which provides a new perspective for the design of a high sensitivity testing system. In this work, the novel LFA platform adopted the AIE carbon dots, which are used to detect SARS-CoV-2-specific IgM and IgG conveniently. Furthermore, this sensor had a low LOD of 100 pg/ml. Therefore, this newly developed strategy has potential applications in the areas of public health for the advancement of clinical research.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042926/fullaggregation-induced emission carbon dotsCOVID-19SARS-CoV-2 antibody detectionlateral flow assayinternal standard calibration |
| spellingShingle | Jian Ju Jian Ju Xinyu Zhang Lin Li Lin Li Sagar Regmi Guoqiang Yang Guoqiang Yang Guoqiang Yang Shixing Tang Shixing Tang Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots Frontiers in Bioengineering and Biotechnology aggregation-induced emission carbon dots COVID-19 SARS-CoV-2 antibody detection lateral flow assay internal standard calibration |
| title | Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots |
| title_full | Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots |
| title_fullStr | Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots |
| title_full_unstemmed | Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots |
| title_short | Development of fluorescent lateral flow immunoassay for SARS-CoV-2-specific IgM and IgG based on aggregation-induced emission carbon dots |
| title_sort | development of fluorescent lateral flow immunoassay for sars cov 2 specific igm and igg based on aggregation induced emission carbon dots |
| topic | aggregation-induced emission carbon dots COVID-19 SARS-CoV-2 antibody detection lateral flow assay internal standard calibration |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042926/full |
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