Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders.
Hepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype va...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3445594?pdf=render |
| _version_ | 1818286942191616000 |
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| author | Annacarmen Petrizzo Maria Lina Tornesello Maria Napolitano Giovanna D'Alessio Angelo Salomone Megna Riccardo Dolcetti Valli De Re Ena Wang Franco M Marincola Franco M Buonaguro Luigi Buonaguro |
| author_facet | Annacarmen Petrizzo Maria Lina Tornesello Maria Napolitano Giovanna D'Alessio Angelo Salomone Megna Riccardo Dolcetti Valli De Re Ena Wang Franco M Marincola Franco M Buonaguro Luigi Buonaguro |
| author_sort | Annacarmen Petrizzo |
| collection | DOAJ |
| description | Hepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation and maturation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative healthy control subjects, with production of Th2-type cytokines. Here, the effect of the recombinant IGKV3-20 protein on human peripheral blood mononuclear cells (PBMCs) from HCV-positive subjects, with known blood levels of cryoglobulins, is shown via gene expression profiling analysis combined to multiparameter flow cytometry and multiplex analyses of cytokines. |
| first_indexed | 2024-12-13T01:32:36Z |
| format | Article |
| id | doaj.art-2f6a5306834349f4a57b82451031c766 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T01:32:36Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2f6a5306834349f4a57b82451031c7662022-12-22T00:03:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4487010.1371/journal.pone.0044870Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders.Annacarmen PetrizzoMaria Lina TorneselloMaria NapolitanoGiovanna D'AlessioAngelo Salomone MegnaRiccardo DolcettiValli De ReEna WangFranco M MarincolaFranco M BuonaguroLuigi BuonaguroHepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation and maturation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative healthy control subjects, with production of Th2-type cytokines. Here, the effect of the recombinant IGKV3-20 protein on human peripheral blood mononuclear cells (PBMCs) from HCV-positive subjects, with known blood levels of cryoglobulins, is shown via gene expression profiling analysis combined to multiparameter flow cytometry and multiplex analyses of cytokines.http://europepmc.org/articles/PMC3445594?pdf=render |
| spellingShingle | Annacarmen Petrizzo Maria Lina Tornesello Maria Napolitano Giovanna D'Alessio Angelo Salomone Megna Riccardo Dolcetti Valli De Re Ena Wang Franco M Marincola Franco M Buonaguro Luigi Buonaguro Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. PLoS ONE |
| title | Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. |
| title_full | Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. |
| title_fullStr | Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. |
| title_full_unstemmed | Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. |
| title_short | Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders. |
| title_sort | multiparametric analyses of human pbmcs loaded ex vivo with a candidate idiotype vaccine for hcv related lymphoproliferative disorders |
| url | http://europepmc.org/articles/PMC3445594?pdf=render |
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