Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?
Methylprednisolone (MP) is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury (SCI). Vitamin C (VC) is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchyma...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2050;epage=2058;aulast=Salem |
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| author | Nesrine Salem Mohamed Y Salem Mohammed M Elmaghrabi Moataz A Elawady Mona A Elawady Dina Sabry Ashraf Shamaa Abdel-Haleem H Elkasapy Noha Ibrhim Azza Elamir |
| author_facet | Nesrine Salem Mohamed Y Salem Mohammed M Elmaghrabi Moataz A Elawady Mona A Elawady Dina Sabry Ashraf Shamaa Abdel-Haleem H Elkasapy Noha Ibrhim Azza Elamir |
| author_sort | Nesrine Salem |
| collection | DOAJ |
| description | Methylprednisolone (MP) is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury (SCI). Vitamin C (VC) is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchymal stem cells (BMMSCs), as multipotent stem cells, are promising candidates in SCI repair. To evaluate the therapeutic effects of MP, VC and BMMSCs on traumatic SCI, 80 adult male rats were randomly divided into seven groups: control, SCI (SCI induction by weight-drop method), MP (SCI induction, followed by administration of 30 mg/kg MP via the tail vein, once every other 6 hours, for five times), VC (SCI induction, followed by intraperitoneal administration of 100 mg/kg VC once a day, for 28 days), MP + VC (SCI induction, followed by administration of MP and VC as the former), BMMSCs (SCI induction, followed by injection of 3 × 106 BMMSCs at the injury site), and BMMSCs + VC (SCI induction, followed by BMMSCs injection and VC administration as the former). Locomotor recovery was assessed using the Basso Mouse Scale. Injured spinal cord tissue was evaluated using hematoxylin-eosin staining and immunohistochemical staining. Expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes was determined using real-time quantitative PCR. BMMSCs intervention better promoted recovery of nerve function of rats with SCI, mitigated nerve cell damage, and decreased expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes than MP and/or VC. More importantly, BMMSCs in combination with VC induced more obvious improvements. These results suggest that VC can enhance the neuroprotective effects of BMMSCs against SCI. |
| first_indexed | 2024-12-13T00:07:58Z |
| format | Article |
| id | doaj.art-2f6c14394d60454bad55d7e6a44c0e90 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-13T00:07:58Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-2f6c14394d60454bad55d7e6a44c0e902022-12-22T00:06:07ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-0112122050205810.4103/1673-5374.221163Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?Nesrine SalemMohamed Y SalemMohammed M ElmaghrabiMoataz A ElawadyMona A ElawadyDina SabryAshraf ShamaaAbdel-Haleem H ElkasapyNoha IbrhimAzza ElamirMethylprednisolone (MP) is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury (SCI). Vitamin C (VC) is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchymal stem cells (BMMSCs), as multipotent stem cells, are promising candidates in SCI repair. To evaluate the therapeutic effects of MP, VC and BMMSCs on traumatic SCI, 80 adult male rats were randomly divided into seven groups: control, SCI (SCI induction by weight-drop method), MP (SCI induction, followed by administration of 30 mg/kg MP via the tail vein, once every other 6 hours, for five times), VC (SCI induction, followed by intraperitoneal administration of 100 mg/kg VC once a day, for 28 days), MP + VC (SCI induction, followed by administration of MP and VC as the former), BMMSCs (SCI induction, followed by injection of 3 × 106 BMMSCs at the injury site), and BMMSCs + VC (SCI induction, followed by BMMSCs injection and VC administration as the former). Locomotor recovery was assessed using the Basso Mouse Scale. Injured spinal cord tissue was evaluated using hematoxylin-eosin staining and immunohistochemical staining. Expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes was determined using real-time quantitative PCR. BMMSCs intervention better promoted recovery of nerve function of rats with SCI, mitigated nerve cell damage, and decreased expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes than MP and/or VC. More importantly, BMMSCs in combination with VC induced more obvious improvements. These results suggest that VC can enhance the neuroprotective effects of BMMSCs against SCI.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2050;epage=2058;aulast=Salemnerve regeneration; spinal cord injury; vitamin C; methylprednisolone; bone marrow mesenchymal stem cells; locotmotor; neural regeneration |
| spellingShingle | Nesrine Salem Mohamed Y Salem Mohammed M Elmaghrabi Moataz A Elawady Mona A Elawady Dina Sabry Ashraf Shamaa Abdel-Haleem H Elkasapy Noha Ibrhim Azza Elamir Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? Neural Regeneration Research nerve regeneration; spinal cord injury; vitamin C; methylprednisolone; bone marrow mesenchymal stem cells; locotmotor; neural regeneration |
| title | Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? |
| title_full | Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? |
| title_fullStr | Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? |
| title_full_unstemmed | Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? |
| title_short | Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury? |
| title_sort | does vitamin c have the ability to augment the therapeutic effect of bone marrow derived mesenchymal stem cells on spinal cord injury |
| topic | nerve regeneration; spinal cord injury; vitamin C; methylprednisolone; bone marrow mesenchymal stem cells; locotmotor; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2050;epage=2058;aulast=Salem |
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