Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus
In Wistar rats, reproductive behavior is controlled in a neural circuit of ventral forebrain including the medial amygdala (Me), bed nucleus of the stria terminalis (BNST) and medial preoptic area (MPOA) via perception of social odors. Diabetes Mellitus (DM) is a widespread metabolic disease that af...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-01-01
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| Series: | Acta Medica Iranica |
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| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/4944 |
| _version_ | 1818640165707448320 |
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| author | Yousef Mohamadi Seyed Behnam-edin Jameie Mohammad Akbari Masumeh Staji Fatemeh Moradi Tahmineh Mokhtari Maryam Khanehzad Gholamreza Hassanzadeh |
| author_facet | Yousef Mohamadi Seyed Behnam-edin Jameie Mohammad Akbari Masumeh Staji Fatemeh Moradi Tahmineh Mokhtari Maryam Khanehzad Gholamreza Hassanzadeh |
| author_sort | Yousef Mohamadi |
| collection | DOAJ |
| description | In Wistar rats, reproductive behavior is controlled in a neural circuit of ventral forebrain including the medial amygdala (Me), bed nucleus of the stria terminalis (BNST) and medial preoptic area (MPOA) via perception of social odors. Diabetes Mellitus (DM) is a widespread metabolic disease that affects many organs in a variety of levels. DM can cause central neuropathies such as neuronal apoptosis, dendritic atrophy, neurochemical alterations and also causes reproductive dysfunctions. So we hypothesized damage to the nuclei of this circuit can cause reproductive dysfunctions. Therefore in this project we assessed diabetic effect on these nuclei. For this purpose neuron tracing technique and TUNEL assay were used. We injected HRP in the MPOA and counted labeled cells in the Me and BNST to evaluate the reduction of neurons in diabetic animals. Also, coronal sections were analyzed with the TMB histochemistry method. Animals in this study were adult male Wistar rats (230 ± 8g) divided to control and 10-week streptozotocin-induced diabetic groups. After data analysis by SPSS 16 software, a significant reduction of HRP-labeled neurons was shown in both Me and BNST nuclei in the diabetic group. Moreover, apoptotic cells were significantly observed in diabetic animals in contrast to control the group. In conclusion, these alterations of the circuit as a result of diabetes might be one of the reasons for reproductive dysfunctions. |
| first_indexed | 2024-12-16T23:06:56Z |
| format | Article |
| id | doaj.art-2f6cb5203ef44a59a53c8bd354a5200b |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-16T23:06:56Z |
| publishDate | 2015-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-2f6cb5203ef44a59a53c8bd354a5200b2022-12-21T22:12:31ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942015-01-015314897Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes MellitusYousef Mohamadi0Seyed Behnam-edin Jameie1Mohammad Akbari2Masumeh Staji3Fatemeh Moradi4Tahmineh Mokhtari5Maryam Khanehzad6Gholamreza Hassanzadeh7Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.In Wistar rats, reproductive behavior is controlled in a neural circuit of ventral forebrain including the medial amygdala (Me), bed nucleus of the stria terminalis (BNST) and medial preoptic area (MPOA) via perception of social odors. Diabetes Mellitus (DM) is a widespread metabolic disease that affects many organs in a variety of levels. DM can cause central neuropathies such as neuronal apoptosis, dendritic atrophy, neurochemical alterations and also causes reproductive dysfunctions. So we hypothesized damage to the nuclei of this circuit can cause reproductive dysfunctions. Therefore in this project we assessed diabetic effect on these nuclei. For this purpose neuron tracing technique and TUNEL assay were used. We injected HRP in the MPOA and counted labeled cells in the Me and BNST to evaluate the reduction of neurons in diabetic animals. Also, coronal sections were analyzed with the TMB histochemistry method. Animals in this study were adult male Wistar rats (230 ± 8g) divided to control and 10-week streptozotocin-induced diabetic groups. After data analysis by SPSS 16 software, a significant reduction of HRP-labeled neurons was shown in both Me and BNST nuclei in the diabetic group. Moreover, apoptotic cells were significantly observed in diabetic animals in contrast to control the group. In conclusion, these alterations of the circuit as a result of diabetes might be one of the reasons for reproductive dysfunctions.https://acta.tums.ac.ir/index.php/acta/article/view/4944Medial preoptic areaMedial amygdalaBed nucleusstria terminalis |
| spellingShingle | Yousef Mohamadi Seyed Behnam-edin Jameie Mohammad Akbari Masumeh Staji Fatemeh Moradi Tahmineh Mokhtari Maryam Khanehzad Gholamreza Hassanzadeh Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus Acta Medica Iranica Medial preoptic area Medial amygdala Bed nucleus stria terminalis |
| title | Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus |
| title_full | Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus |
| title_fullStr | Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus |
| title_full_unstemmed | Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus |
| title_short | Hyperglycemia Decreased Medial Amygdala Projections to Medial Preoptic Area in Experimental Model of Diabetes Mellitus |
| title_sort | hyperglycemia decreased medial amygdala projections to medial preoptic area in experimental model of diabetes mellitus |
| topic | Medial preoptic area Medial amygdala Bed nucleus stria terminalis |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/4944 |
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