Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers
Abstract Objective Donor liver function in transplantation is defined by mitochondrial function and the ability of mitochondria to recover from the sequence of warm and/or cold ischemia. Mitochondrial resilience maybe related to assembly and- subunit composition of Complex 1. The aim of this study w...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2863-7 |
| _version_ | 1819063196308209664 |
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| author | S. E. Khorsandi J. W. Taanman N. Heaton |
| author_facet | S. E. Khorsandi J. W. Taanman N. Heaton |
| author_sort | S. E. Khorsandi |
| collection | DOAJ |
| description | Abstract Objective Donor liver function in transplantation is defined by mitochondrial function and the ability of mitochondria to recover from the sequence of warm and/or cold ischemia. Mitochondrial resilience maybe related to assembly and- subunit composition of Complex 1. The aim of this study was to determine if Complex 1 subunit composition was different in donor livers of varying quality and whether oxygen exposure had any effect. Results Five human livers not suitable for transplant were split. One half placed in cold static storage and the other half exposed to 40% oxygen for 2 h. Protein was extracted for western blot. Membranes were probed with antibodies against β-actin and the following subunits of Complex 1: MTND1, NDUFA10, NDUFB6 and NDUFV2. No difference in steady state Complex 1 subunit composition was demonstrated between donor livers of varying quality, in terms of steatosis or mode of donation. Neither did exposure to oxygen influence Complex 1 subunit composition. This small observational study on subunit levels suggest that Complex 1 is fully assembled as no degradation of subunits associated with the different parts of the enzyme was seen. |
| first_indexed | 2024-12-21T15:10:50Z |
| format | Article |
| id | doaj.art-2f6d6cdc08d642088e9eb985a711981f |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-21T15:10:50Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-2f6d6cdc08d642088e9eb985a711981f2022-12-21T18:59:18ZengBMCBMC Research Notes1756-05002017-11-011011610.1186/s13104-017-2863-7Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor liversS. E. Khorsandi0J. W. Taanman1N. Heaton2Institute of Liver Studies, King’s College Hospital, Kings College LondonDepartment of Clinical Neurosciences, Institute of Neurology, University College LondonInstitute of Liver Studies, King’s College Hospital, Kings College LondonAbstract Objective Donor liver function in transplantation is defined by mitochondrial function and the ability of mitochondria to recover from the sequence of warm and/or cold ischemia. Mitochondrial resilience maybe related to assembly and- subunit composition of Complex 1. The aim of this study was to determine if Complex 1 subunit composition was different in donor livers of varying quality and whether oxygen exposure had any effect. Results Five human livers not suitable for transplant were split. One half placed in cold static storage and the other half exposed to 40% oxygen for 2 h. Protein was extracted for western blot. Membranes were probed with antibodies against β-actin and the following subunits of Complex 1: MTND1, NDUFA10, NDUFB6 and NDUFV2. No difference in steady state Complex 1 subunit composition was demonstrated between donor livers of varying quality, in terms of steatosis or mode of donation. Neither did exposure to oxygen influence Complex 1 subunit composition. This small observational study on subunit levels suggest that Complex 1 is fully assembled as no degradation of subunits associated with the different parts of the enzyme was seen.http://link.springer.com/article/10.1186/s13104-017-2863-7MitochondriaLiver transplantationComplex 1OxygenDonor after cardiac death |
| spellingShingle | S. E. Khorsandi J. W. Taanman N. Heaton Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers BMC Research Notes Mitochondria Liver transplantation Complex 1 Oxygen Donor after cardiac death |
| title | Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| title_full | Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| title_fullStr | Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| title_full_unstemmed | Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| title_short | Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| title_sort | subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers |
| topic | Mitochondria Liver transplantation Complex 1 Oxygen Donor after cardiac death |
| url | http://link.springer.com/article/10.1186/s13104-017-2863-7 |
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