Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle
The fruits of Ailanthus altissima Swingle (AS) possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. In in vitro studies, AS revealed the strong reducing power and DPPH scavenging effect, but hydroxyl radical scaven...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.784898/full |
| _version_ | 1818459749327306752 |
|---|---|
| author | Ya-nan Mo Feng Cheng Feng Cheng Zhen Yang Xiao-fei Shang Jian-ping Liang Ruo-feng Shang Bao-cheng Hao Xue-hong Wang Hong-juan Zhang Ahmidin Wali Chun-fang Lu Yu Liu Yu Liu |
| author_facet | Ya-nan Mo Feng Cheng Feng Cheng Zhen Yang Xiao-fei Shang Jian-ping Liang Ruo-feng Shang Bao-cheng Hao Xue-hong Wang Hong-juan Zhang Ahmidin Wali Chun-fang Lu Yu Liu Yu Liu |
| author_sort | Ya-nan Mo |
| collection | DOAJ |
| description | The fruits of Ailanthus altissima Swingle (AS) possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. In in vitro studies, AS revealed the strong reducing power and DPPH scavenging effect, but hydroxyl radical scavenging activity and ferrous ions-chelating ability were not strong. Meanwhile, the oxidative stress RAW264.7 cell injury model was established, the low and medium-doses of AS showed significant protective effects on the viability of H2O2-treated cells by CCK-8 method. Besides, three doses of AS all increased the activities of SOD, CAT, and GSH-Px and decreased the MDA level compared with the H2O2 group, suggesting it significantly relieved oxidative stress of cells. The active ingredients and related targets of AS were collected by HERB and Swiss Target Prediction database, the common targets of drugs and diseases database were conducted by GeneCards database platform and the Venny platform. We screened the core targets of AS like threonine kinase1 (AKT1), mitogen-activated protein kinase 1 (MAPK1), sirtuin-1 (SIRT1), mechanistic target of rapamycin kinase (MTOR) by STRING database, and the key pathways involved PI3K-AKT and FoxO signaling pathway by KEGG pathway enrichment analysis. Besides, qRT-PCR revealed AS preconditioning significantly up-regulated the expression level of AKT1, SIRT1, MAPK1, and MTOR in model cells, and the effect was related to the regulation of FoxO and PI3K/AKT signaling pathway. In summary, AS showed significant antioxidant activity and its potential mechanism was regulating FoxO and PI3K/AKT signaling pathway. |
| first_indexed | 2024-12-14T23:19:18Z |
| format | Article |
| id | doaj.art-2f71dfc624f24c6cb757effe08b45c5e |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-14T23:19:18Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2f71dfc624f24c6cb757effe08b45c5e2022-12-21T22:44:00ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-12-01810.3389/fvets.2021.784898784898Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima SwingleYa-nan Mo0Feng Cheng1Feng Cheng2Zhen Yang3Xiao-fei Shang4Jian-ping Liang5Ruo-feng Shang6Bao-cheng Hao7Xue-hong Wang8Hong-juan Zhang9Ahmidin Wali10Chun-fang Lu11Yu Liu12Yu Liu13Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaKey Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, ChinaKey Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaThe fruits of Ailanthus altissima Swingle (AS) possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. In in vitro studies, AS revealed the strong reducing power and DPPH scavenging effect, but hydroxyl radical scavenging activity and ferrous ions-chelating ability were not strong. Meanwhile, the oxidative stress RAW264.7 cell injury model was established, the low and medium-doses of AS showed significant protective effects on the viability of H2O2-treated cells by CCK-8 method. Besides, three doses of AS all increased the activities of SOD, CAT, and GSH-Px and decreased the MDA level compared with the H2O2 group, suggesting it significantly relieved oxidative stress of cells. The active ingredients and related targets of AS were collected by HERB and Swiss Target Prediction database, the common targets of drugs and diseases database were conducted by GeneCards database platform and the Venny platform. We screened the core targets of AS like threonine kinase1 (AKT1), mitogen-activated protein kinase 1 (MAPK1), sirtuin-1 (SIRT1), mechanistic target of rapamycin kinase (MTOR) by STRING database, and the key pathways involved PI3K-AKT and FoxO signaling pathway by KEGG pathway enrichment analysis. Besides, qRT-PCR revealed AS preconditioning significantly up-regulated the expression level of AKT1, SIRT1, MAPK1, and MTOR in model cells, and the effect was related to the regulation of FoxO and PI3K/AKT signaling pathway. In summary, AS showed significant antioxidant activity and its potential mechanism was regulating FoxO and PI3K/AKT signaling pathway.https://www.frontiersin.org/articles/10.3389/fvets.2021.784898/fullAilanthus altissima Swingleantioxidant activityRAW264.7 cellnetwork pharmacologyin vitroantioxidant mechanism |
| spellingShingle | Ya-nan Mo Feng Cheng Feng Cheng Zhen Yang Xiao-fei Shang Jian-ping Liang Ruo-feng Shang Bao-cheng Hao Xue-hong Wang Hong-juan Zhang Ahmidin Wali Chun-fang Lu Yu Liu Yu Liu Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle Frontiers in Veterinary Science Ailanthus altissima Swingle antioxidant activity RAW264.7 cell network pharmacology in vitro antioxidant mechanism |
| title | Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle |
| title_full | Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle |
| title_fullStr | Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle |
| title_full_unstemmed | Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle |
| title_short | Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle |
| title_sort | antioxidant activity and the potential mechanism of the fruit from ailanthus altissima swingle |
| topic | Ailanthus altissima Swingle antioxidant activity RAW264.7 cell network pharmacology in vitro antioxidant mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.784898/full |
| work_keys_str_mv | AT yananmo antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT fengcheng antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT fengcheng antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT zhenyang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT xiaofeishang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT jianpingliang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT ruofengshang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT baochenghao antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT xuehongwang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT hongjuanzhang antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT ahmidinwali antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT chunfanglu antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT yuliu antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle AT yuliu antioxidantactivityandthepotentialmechanismofthefruitfromailanthusaltissimaswingle |