A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone
A binding study of nickel ions by a new recombinant human Growth Hormone (hGH), produced as an injected drug, has been done at 27˚C in NaCl solution (50 mM) using an isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for nickel ions. The intrinsic d...
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2010-12-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_6397_916950c3c45b35ab9e7739e6a7bb308b.pdf |
| _version_ | 1811267488467386368 |
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| author | Ali Akbar Saboury Safoura Amiri |
| author_facet | Ali Akbar Saboury Safoura Amiri |
| author_sort | Ali Akbar Saboury |
| collection | DOAJ |
| description | A binding study of nickel ions by a new recombinant human Growth Hormone (hGH), produced as an injected drug, has been done at 27˚C in NaCl solution (50 mM) using an isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for nickel ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μM and -16.5 kJ/mol, respectively. Thermodynamic parameters of nickel ion binding are compared to the other metal ions. The molar entropy of binding is 29.3 J K-1 mol-1 for Ni2+, less than Cu2+ and more than other metal ions, means that the disorder of the protein structure due to the binding of nickel ions is more than to the other ion metals, except Cu2+. It is expected that nickel ions can prevent from the aggregation of the protein. |
| first_indexed | 2024-04-12T21:03:28Z |
| format | Article |
| id | doaj.art-2f71f64f747e4d96beb68f5b403e528d |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-04-12T21:03:28Z |
| publishDate | 2010-12-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-2f71f64f747e4d96beb68f5b403e528d2022-12-22T03:16:46ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862010-12-0129431366397A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth HormoneAli Akbar Saboury0Safoura Amiri1Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, I.R. IRANInstitute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, I.R. IRANA binding study of nickel ions by a new recombinant human Growth Hormone (hGH), produced as an injected drug, has been done at 27˚C in NaCl solution (50 mM) using an isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for nickel ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μM and -16.5 kJ/mol, respectively. Thermodynamic parameters of nickel ion binding are compared to the other metal ions. The molar entropy of binding is 29.3 J K-1 mol-1 for Ni2+, less than Cu2+ and more than other metal ions, means that the disorder of the protein structure due to the binding of nickel ions is more than to the other ion metals, except Cu2+. It is expected that nickel ions can prevent from the aggregation of the protein.http://www.ijcce.ac.ir/article_6397_916950c3c45b35ab9e7739e6a7bb308b.pdfhuman growth hormonenickel ionmetal bindingtitration calorimetry |
| spellingShingle | Ali Akbar Saboury Safoura Amiri A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone Iranian Journal of Chemistry & Chemical Engineering human growth hormone nickel ion metal binding titration calorimetry |
| title | A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone |
| title_full | A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone |
| title_fullStr | A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone |
| title_full_unstemmed | A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone |
| title_short | A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone |
| title_sort | microcalorimetry study of the binding of nickel ion by human growth hormone |
| topic | human growth hormone nickel ion metal binding titration calorimetry |
| url | http://www.ijcce.ac.ir/article_6397_916950c3c45b35ab9e7739e6a7bb308b.pdf |
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