Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice.
Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matri...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3767600?pdf=render |
| _version_ | 1819099109054742528 |
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| author | Betty Fumel Stéphanie Roy Sophie Fouchécourt Gabriel Livera Anne-Simone Parent François Casas Florian Guillou |
| author_facet | Betty Fumel Stéphanie Roy Sophie Fouchécourt Gabriel Livera Anne-Simone Parent François Casas Florian Guillou |
| author_sort | Betty Fumel |
| collection | DOAJ |
| description | Among T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43-/- mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43-/- probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43. |
| first_indexed | 2024-12-22T00:41:39Z |
| format | Article |
| id | doaj.art-2f7320ff34d847a7ae82340fdc734dc8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T00:41:39Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2f7320ff34d847a7ae82340fdc734dc82022-12-21T18:44:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7401510.1371/journal.pone.0074015Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice.Betty FumelStéphanie RoySophie FouchécourtGabriel LiveraAnne-Simone ParentFrançois CasasFlorian GuillouAmong T3 receptors, TRα1 is ubiquitous and its deletion or a specific expression of a dominant-negative TRα1 isoform in Sertoli cell leads to an increase in testis weight and sperm production. The identification of a 43-kDa truncated form of the nuclear receptor TRα1 (p43) in the mitochondrial matrix led us to test the hypothesis that this mitochondrial transcription factor could regulate Sertoli cell proliferation. Here we report that p43 depletion in mice increases testis weight and sperm reserve. In addition, we found that p43 deletion increases Sertoli cell proliferation in postnatal testis at 3 days of development. Electron microscopy studies evidence an alteration of mitochondrial morphology observed specifically in Sertoli cells of p43-/- mice. Moreover, gene expression studies indicate that the lack of p43 in testis induced an alteration of the mitochondrial-nuclear cross-talk. In particular, the up-regulation of Cdk4 and c-myc pathway in p43-/- probably explain the extended proliferation recorded in Sertoli cells of these mice. Our finding suggests that T3 limits post-natal Sertoli cell proliferation mainly through its mitochondrial T3 receptor p43.http://europepmc.org/articles/PMC3767600?pdf=render |
| spellingShingle | Betty Fumel Stéphanie Roy Sophie Fouchécourt Gabriel Livera Anne-Simone Parent François Casas Florian Guillou Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. PLoS ONE |
| title | Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. |
| title_full | Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. |
| title_fullStr | Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. |
| title_full_unstemmed | Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. |
| title_short | Depletion of the p43 mitochondrial T3 receptor increases Sertoli cell proliferation in mice. |
| title_sort | depletion of the p43 mitochondrial t3 receptor increases sertoli cell proliferation in mice |
| url | http://europepmc.org/articles/PMC3767600?pdf=render |
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