Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus
Abstract Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CaLas), is the most serious disease worldwide. CaLasSDE460 was previously characterized as a potential virulence factor of CaLas. However, the function and mechanism of CaLasSDE460 involved in CaLas against citrus is s...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Molecular Horticulture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43897-023-00062-3 |
| _version_ | 1797769289299656704 |
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| author | Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou |
| author_facet | Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou |
| author_sort | Shuai Wang |
| collection | DOAJ |
| description | Abstract Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CaLas), is the most serious disease worldwide. CaLasSDE460 was previously characterized as a potential virulence factor of CaLas. However, the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still elusive. Here, we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges (C. sinensis Osbeck) contributed to the early growth of CaLas and the development of symptoms. When the temperature increased from 25 °C to 32 °C, CaLas growth and symptom development in transgenic plants were slower than those in WT controls. RNA-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological processes. At 25 °C, transcription activities of the “Protein processing in endoplasmic reticulum” and “Cyanoamino acid metabolism” pathways increased while transcription activities of many pathways decreased at 32 °C. 124 and 53 genes, separately annotated to plant-pathogen interaction and MAPK signaling pathways, showed decreased expression at 32 °C, compared with these (38 for plant-pathogen interaction and 17 for MAPK signaling) at 25 °C. Several important genes (MAPKKK14, HSP70b, NCED3 and WRKY33), remarkably affected by CaLasSDE460, were identified. Totally, our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent. Graphical Abstract |
| first_indexed | 2024-03-12T21:06:44Z |
| format | Article |
| id | doaj.art-2f73ce5cc9614e73b03286b888dde725 |
| institution | Directory Open Access Journal |
| issn | 2730-9401 |
| language | English |
| last_indexed | 2024-03-12T21:06:44Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Horticulture |
| spelling | doaj.art-2f73ce5cc9614e73b03286b888dde7252023-07-30T11:24:45ZengBMCMolecular Horticulture2730-94012023-07-013111710.1186/s43897-023-00062-3Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrusShuai Wang0Meixia Du1Liting Dong2Rongrong Qu3Danlu Ran4Juanjuan Ma5Xuefeng Wang6Lanzhen Xu7Weimin Li8Yongrui He9Xiuping Zou10Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterKey Laboratory for Northern Urban, Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of AgricultureIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterAbstract Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CaLas), is the most serious disease worldwide. CaLasSDE460 was previously characterized as a potential virulence factor of CaLas. However, the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still elusive. Here, we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges (C. sinensis Osbeck) contributed to the early growth of CaLas and the development of symptoms. When the temperature increased from 25 °C to 32 °C, CaLas growth and symptom development in transgenic plants were slower than those in WT controls. RNA-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological processes. At 25 °C, transcription activities of the “Protein processing in endoplasmic reticulum” and “Cyanoamino acid metabolism” pathways increased while transcription activities of many pathways decreased at 32 °C. 124 and 53 genes, separately annotated to plant-pathogen interaction and MAPK signaling pathways, showed decreased expression at 32 °C, compared with these (38 for plant-pathogen interaction and 17 for MAPK signaling) at 25 °C. Several important genes (MAPKKK14, HSP70b, NCED3 and WRKY33), remarkably affected by CaLasSDE460, were identified. Totally, our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent. Graphical Abstracthttps://doi.org/10.1186/s43897-023-00062-3CitrusHLBCaLasSDE460TranscriptomeEctopic expression |
| spellingShingle | Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus Molecular Horticulture Citrus HLB CaLasSDE460 Transcriptome Ectopic expression |
| title | Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus |
| title_full | Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus |
| title_fullStr | Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus |
| title_full_unstemmed | Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus |
| title_short | Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of “Candidatus Liberibacter asiaticus” in citrus |
| title_sort | function and molecular mechanism analysis of calassde460 effector involved in the pathogenesis of candidatus liberibacter asiaticus in citrus |
| topic | Citrus HLB CaLasSDE460 Transcriptome Ectopic expression |
| url | https://doi.org/10.1186/s43897-023-00062-3 |
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