In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells
Strategies that alter the pH of wounds to improve healing outcomes are an emerging area of interest. Currently, there is limited understanding of the effect of hydrogen (H<sup>+</sup>) on the functionality of skin cells during proliferation and migration, highlighting the need for resear...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/13/7188 |
| _version_ | 1797479706252017664 |
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| author | Pivian Sim Yunmei Song Gink N. Yang Allison J. Cowin Sanjay Garg |
| author_facet | Pivian Sim Yunmei Song Gink N. Yang Allison J. Cowin Sanjay Garg |
| author_sort | Pivian Sim |
| collection | DOAJ |
| description | Strategies that alter the pH of wounds to improve healing outcomes are an emerging area of interest. Currently, there is limited understanding of the effect of hydrogen (H<sup>+</sup>) on the functionality of skin cells during proliferation and migration, highlighting the need for research to determine the effect of pH during wound healing. This study aimed to determine the effect of acidification on the metabolic activity and migration of human immortalized keratinocytes (HaCaT) and human foreskin fibroblasts (HFF). In vitro models were used with phosphoric and citric acid buffers at a pH range between 3 and 7. Our results showed that cells were more viable in buffers with low rather than high ionic strength. A time-dependent effect of the acidification treatment was also observed with cell metabolic activity varying with treatment duration and frequency. Our results showed that a 24 h treatment and subsequent resting phase significantly improved cell proliferation and migration. This in vitro study is the first to establish a correlation between the role of acidic pH, molarity and treatment regimen in cellular activity. Our data demonstrated a positive effect of acidic pH on cell metabolic activity and migration rate, suggesting a clinical potential in indications such as wound healing. |
| first_indexed | 2024-03-09T21:49:39Z |
| format | Article |
| id | doaj.art-2f77cdfc15924800b95e24c6c574c442 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:49:39Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2f77cdfc15924800b95e24c6c574c4422023-11-23T20:09:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313718810.3390/ijms23137188In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin CellsPivian Sim0Yunmei Song1Gink N. Yang2Allison J. Cowin3Sanjay Garg4Centre for Pharmaceutical Innovation (CPI)Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, AustraliaCentre for Pharmaceutical Innovation (CPI)Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, AustraliaRegenerative Medicine, Future Industries Institute, University of South Australia, Adelaide, SA 5095, AustraliaRegenerative Medicine, Future Industries Institute, University of South Australia, Adelaide, SA 5095, AustraliaCentre for Pharmaceutical Innovation (CPI)Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, AustraliaStrategies that alter the pH of wounds to improve healing outcomes are an emerging area of interest. Currently, there is limited understanding of the effect of hydrogen (H<sup>+</sup>) on the functionality of skin cells during proliferation and migration, highlighting the need for research to determine the effect of pH during wound healing. This study aimed to determine the effect of acidification on the metabolic activity and migration of human immortalized keratinocytes (HaCaT) and human foreskin fibroblasts (HFF). In vitro models were used with phosphoric and citric acid buffers at a pH range between 3 and 7. Our results showed that cells were more viable in buffers with low rather than high ionic strength. A time-dependent effect of the acidification treatment was also observed with cell metabolic activity varying with treatment duration and frequency. Our results showed that a 24 h treatment and subsequent resting phase significantly improved cell proliferation and migration. This in vitro study is the first to establish a correlation between the role of acidic pH, molarity and treatment regimen in cellular activity. Our data demonstrated a positive effect of acidic pH on cell metabolic activity and migration rate, suggesting a clinical potential in indications such as wound healing.https://www.mdpi.com/1422-0067/23/13/7188acidicbufferionic strengthpHproliferationmetabolic activity |
| spellingShingle | Pivian Sim Yunmei Song Gink N. Yang Allison J. Cowin Sanjay Garg In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells International Journal of Molecular Sciences acidic buffer ionic strength pH proliferation metabolic activity |
| title | In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells |
| title_full | In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells |
| title_fullStr | In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells |
| title_full_unstemmed | In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells |
| title_short | In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells |
| title_sort | in vitro wound healing properties of novel acidic treatment regimen in enhancing metabolic activity and migration of skin cells |
| topic | acidic buffer ionic strength pH proliferation metabolic activity |
| url | https://www.mdpi.com/1422-0067/23/13/7188 |
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