Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer
Minichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.852135/full |
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| author | Jing Yuan Hua Lan Dongqing Huang Dongqing Huang Xiaohui Guo Chu Liu Shuping Liu Peng Zhang Yan Cheng Songshu Xiao |
| author_facet | Jing Yuan Hua Lan Dongqing Huang Dongqing Huang Xiaohui Guo Chu Liu Shuping Liu Peng Zhang Yan Cheng Songshu Xiao |
| author_sort | Jing Yuan |
| collection | DOAJ |
| description | Minichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in cell proliferation and cancer development. However, few articles have systematically reported the pathogenic roles of MCM2 across cancers. Therefore, the present pan-cancer study was conducted. Various computational tools were used to investigate the MCM2 expression level, genetic mutation rate, and regulating mechanism, immune infiltration, tumor diagnosis and prognosis, therapeutic response and drug sensitivity of various cancers. The expression and function of MCM2 were examined by Western blotting and CCK-8 assays. MCM2 was significantly upregulated in almost all cancers and cancer subtypes in The Cancer Genome Atlas and was closely associated with tumor mutation burden, tumor stage, and immune therapy response. Upregulation of MCM2 expression may be correlated with a high level of alterations rate. MCM2 expression was associated with the infiltration of various immune cells and molecules and markedly associated with a poor prognosis. Western blotting and CCK-8 assays revealed that MCM2 expression was significantly upregulated in melanoma cell lines. Our results also suggested that MCM2 promotes cell proliferation in vitro by activating cell proliferation pathways such as the Akt signaling pathways. This study explored the oncogenic role of MCM2 across cancers, provided data on the underlying mechanisms of these cancers for further research and demonstrated that MCM2 may be a promising target for cancer immunotherapy. |
| first_indexed | 2024-04-12T17:22:18Z |
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| id | doaj.art-2f77ffdc83d14b3b976e4d9283b62110 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-12T17:22:18Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-2f77ffdc83d14b3b976e4d9283b621102022-12-22T03:23:26ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-05-011010.3389/fcell.2022.852135852135Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-CancerJing Yuan0Hua Lan1Dongqing Huang2Dongqing Huang3Xiaohui Guo4Chu Liu5Shuping Liu6Peng Zhang7Yan Cheng8Songshu Xiao9Department of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Gynecology, The Second Hospital of Zhuzhou, Zhuzhou, ChinaDepartment of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Rehabilitation, Changsha Central Hospital of University of South China, Changsha, ChinaGraduate Collaborative Training Base of the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, ChinaDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Gynecology and Obstetrics, Third Xiangya Hospital, Central South University, Changsha, ChinaMinichromosome maintenance 2 (MCM2) is a member of the minichromosomal maintenance family of proteins that mainly regulates DNA replication and the cell cycle and is involved in regulating cancer cell proliferation in various cancers. Previous studies have reported that MCM2 plays a pivotal role in cell proliferation and cancer development. However, few articles have systematically reported the pathogenic roles of MCM2 across cancers. Therefore, the present pan-cancer study was conducted. Various computational tools were used to investigate the MCM2 expression level, genetic mutation rate, and regulating mechanism, immune infiltration, tumor diagnosis and prognosis, therapeutic response and drug sensitivity of various cancers. The expression and function of MCM2 were examined by Western blotting and CCK-8 assays. MCM2 was significantly upregulated in almost all cancers and cancer subtypes in The Cancer Genome Atlas and was closely associated with tumor mutation burden, tumor stage, and immune therapy response. Upregulation of MCM2 expression may be correlated with a high level of alterations rate. MCM2 expression was associated with the infiltration of various immune cells and molecules and markedly associated with a poor prognosis. Western blotting and CCK-8 assays revealed that MCM2 expression was significantly upregulated in melanoma cell lines. Our results also suggested that MCM2 promotes cell proliferation in vitro by activating cell proliferation pathways such as the Akt signaling pathways. This study explored the oncogenic role of MCM2 across cancers, provided data on the underlying mechanisms of these cancers for further research and demonstrated that MCM2 may be a promising target for cancer immunotherapy.https://www.frontiersin.org/articles/10.3389/fcell.2022.852135/fullMCM2comprehensive bioinformaticsimmune therapy responseprognostic valueskin cutaneous melanoma |
| spellingShingle | Jing Yuan Hua Lan Dongqing Huang Dongqing Huang Xiaohui Guo Chu Liu Shuping Liu Peng Zhang Yan Cheng Songshu Xiao Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer Frontiers in Cell and Developmental Biology MCM2 comprehensive bioinformatics immune therapy response prognostic value skin cutaneous melanoma |
| title | Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer |
| title_full | Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer |
| title_fullStr | Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer |
| title_full_unstemmed | Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer |
| title_short | Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer |
| title_sort | multi omics analysis of mcm2 as a promising biomarker in pan cancer |
| topic | MCM2 comprehensive bioinformatics immune therapy response prognostic value skin cutaneous melanoma |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.852135/full |
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