Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats
BackgroundVagus nerve is one of the crucial routes in communication between the immune and central nervous systems. The impaired vagal nerve function may intensify peripheral inflammatory processes. This effect subsides along with prolonged recovery after permanent nerve injury. One of the results o...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.869526/full |
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| author | Anna Teresa Kobrzycka Adrian Mateusz Stankiewicz Joanna Goscik Monika Gora Beata Burzynska Roksana Iwanicka-Nowicka Roksana Iwanicka-Nowicka Krystyna Pierzchala-Koziec Marek Wieczorek |
| author_facet | Anna Teresa Kobrzycka Adrian Mateusz Stankiewicz Joanna Goscik Monika Gora Beata Burzynska Roksana Iwanicka-Nowicka Roksana Iwanicka-Nowicka Krystyna Pierzchala-Koziec Marek Wieczorek |
| author_sort | Anna Teresa Kobrzycka |
| collection | DOAJ |
| description | BackgroundVagus nerve is one of the crucial routes in communication between the immune and central nervous systems. The impaired vagal nerve function may intensify peripheral inflammatory processes. This effect subsides along with prolonged recovery after permanent nerve injury. One of the results of such compensation is a normalized plasma concentration of stress hormone corticosterone – a marker of hypothalamic-pituitary-adrenal (HPA) axis activity. In this work, we strive to explain this corticosterone normalization by studying the mechanisms responsible for compensation-related neurochemical alterations in the hypothalamus.Materials and MethodsUsing microarrays and high performance liquid chromatography (HPLC), we measured genome-wide gene expression and major amino acid neurotransmitters content in the hypothalamus of bilaterally vagotomized rats, 1 month after surgery.ResultsOur results show that, in the long term, vagotomy affects hypothalamic amino acids concentration but not mRNA expression of tested genes.DiscussionWe propose an alternative pathway of immune to CNS communication after vagotomy, leading to activation of the HPA axis, by influencing central amino acids and subsequent monoaminergic neurotransmission. |
| first_indexed | 2024-04-13T22:09:49Z |
| format | Article |
| id | doaj.art-2f799512b51f46ea97bb377562abadc3 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-13T22:09:49Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-2f799512b51f46ea97bb377562abadc32022-12-22T02:27:47ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-07-011610.3389/fnbeh.2022.869526869526Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized RatsAnna Teresa Kobrzycka0Adrian Mateusz Stankiewicz1Joanna Goscik2Monika Gora3Beata Burzynska4Roksana Iwanicka-Nowicka5Roksana Iwanicka-Nowicka6Krystyna Pierzchala-Koziec7Marek Wieczorek8Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Łodz, Łodz, PolandDepartment of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, PolandSoftware Department, Faculty of Computer Science, Bialystok University of Technology, Bialystok, PolandDepartment of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandFaculty of Biology, University of Warsaw, Warsaw, PolandDepartment of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Krakow, PolandDepartment of Neurobiology, Faculty of Biology and Environmental Protection, University of Łodz, Łodz, PolandBackgroundVagus nerve is one of the crucial routes in communication between the immune and central nervous systems. The impaired vagal nerve function may intensify peripheral inflammatory processes. This effect subsides along with prolonged recovery after permanent nerve injury. One of the results of such compensation is a normalized plasma concentration of stress hormone corticosterone – a marker of hypothalamic-pituitary-adrenal (HPA) axis activity. In this work, we strive to explain this corticosterone normalization by studying the mechanisms responsible for compensation-related neurochemical alterations in the hypothalamus.Materials and MethodsUsing microarrays and high performance liquid chromatography (HPLC), we measured genome-wide gene expression and major amino acid neurotransmitters content in the hypothalamus of bilaterally vagotomized rats, 1 month after surgery.ResultsOur results show that, in the long term, vagotomy affects hypothalamic amino acids concentration but not mRNA expression of tested genes.DiscussionWe propose an alternative pathway of immune to CNS communication after vagotomy, leading to activation of the HPA axis, by influencing central amino acids and subsequent monoaminergic neurotransmission.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.869526/fullhypothalamussubdiaphragmatic vagotomyamino acid neurotransmittersinflammatory responsemicroarrayHPA axis |
| spellingShingle | Anna Teresa Kobrzycka Adrian Mateusz Stankiewicz Joanna Goscik Monika Gora Beata Burzynska Roksana Iwanicka-Nowicka Roksana Iwanicka-Nowicka Krystyna Pierzchala-Koziec Marek Wieczorek Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats Frontiers in Behavioral Neuroscience hypothalamus subdiaphragmatic vagotomy amino acid neurotransmitters inflammatory response microarray HPA axis |
| title | Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats |
| title_full | Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats |
| title_fullStr | Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats |
| title_full_unstemmed | Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats |
| title_short | Hypothalamic Neurochemical Changes in Long-Term Recovered Bilateral Subdiaphragmatic Vagotomized Rats |
| title_sort | hypothalamic neurochemical changes in long term recovered bilateral subdiaphragmatic vagotomized rats |
| topic | hypothalamus subdiaphragmatic vagotomy amino acid neurotransmitters inflammatory response microarray HPA axis |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.869526/full |
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