Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro
Nanoparticles can play valuable roles in delivering nucleic acids, including microRNAs (miRNA), which are small, non-coding RNA segments. In this way, nanoparticles may exert post-transcriptional regulatory influences on various inflammatory conditions and bone disorders. This study used biocompatib...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/11/1755 |
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| author | Sepanta Hosseinpour Huan Dai Laurence J. Walsh Chun Xu |
| author_facet | Sepanta Hosseinpour Huan Dai Laurence J. Walsh Chun Xu |
| author_sort | Sepanta Hosseinpour |
| collection | DOAJ |
| description | Nanoparticles can play valuable roles in delivering nucleic acids, including microRNAs (miRNA), which are small, non-coding RNA segments. In this way, nanoparticles may exert post-transcriptional regulatory influences on various inflammatory conditions and bone disorders. This study used biocompatible, core–cone-structured, mesoporous silica nanoparticles (MSN-CC) to deliver miRNA-26a to macrophages in order to influence osteogenesis in vitro. The loaded nanoparticles (MSN-CC-miRNA-26) showed low-level toxicity towards macrophages (RAW 264.7 cells) and were internalized efficiently, causing the reduced expression of pro-inflammatory cytokines, as seen via real-time PCR and cytokine immunoassays. The conditioned macrophages created a favorable osteoimmune environment for MC3T3-E1 preosteoblasts, driving osteogenic differentiation with enhanced osteogenic marker expression, alkaline phosphatase (ALP) production, extracellular matrix formation, and calcium deposition. An indirect co-culture system revealed that direct osteogenic induction and immunomodulation by MSN-CC-miRNA-26a synergistically increased bone production due to the crosstalk between MSN-CC-miRNA-26a-conditioned macrophages and MSN-CC-miRNA-26a-treated preosteoblasts. These findings demonstrate the value of nanoparticle delivery of miR-NA-26a using MSN-CC for suppressing the production of pro-inflammatory cytokines with macrophages and for driving osteogenic differentiation in preosteoblasts via osteoimmune modulation. |
| first_indexed | 2024-03-11T03:00:28Z |
| format | Article |
| id | doaj.art-2f7cc6503547461cbc977ad6f48022d1 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T03:00:28Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-2f7cc6503547461cbc977ad6f48022d12023-11-18T08:19:05ZengMDPI AGNanomaterials2079-49912023-05-011311175510.3390/nano13111755Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In VitroSepanta Hosseinpour0Huan Dai1Laurence J. Walsh2Chun Xu3School of Dentistry, The University of Queensland, Herston, QLD 4006, AustraliaSchool of Dentistry, The University of Queensland, Herston, QLD 4006, AustraliaSchool of Dentistry, The University of Queensland, Herston, QLD 4006, AustraliaSchool of Dentistry, The University of Queensland, Herston, QLD 4006, AustraliaNanoparticles can play valuable roles in delivering nucleic acids, including microRNAs (miRNA), which are small, non-coding RNA segments. In this way, nanoparticles may exert post-transcriptional regulatory influences on various inflammatory conditions and bone disorders. This study used biocompatible, core–cone-structured, mesoporous silica nanoparticles (MSN-CC) to deliver miRNA-26a to macrophages in order to influence osteogenesis in vitro. The loaded nanoparticles (MSN-CC-miRNA-26) showed low-level toxicity towards macrophages (RAW 264.7 cells) and were internalized efficiently, causing the reduced expression of pro-inflammatory cytokines, as seen via real-time PCR and cytokine immunoassays. The conditioned macrophages created a favorable osteoimmune environment for MC3T3-E1 preosteoblasts, driving osteogenic differentiation with enhanced osteogenic marker expression, alkaline phosphatase (ALP) production, extracellular matrix formation, and calcium deposition. An indirect co-culture system revealed that direct osteogenic induction and immunomodulation by MSN-CC-miRNA-26a synergistically increased bone production due to the crosstalk between MSN-CC-miRNA-26a-conditioned macrophages and MSN-CC-miRNA-26a-treated preosteoblasts. These findings demonstrate the value of nanoparticle delivery of miR-NA-26a using MSN-CC for suppressing the production of pro-inflammatory cytokines with macrophages and for driving osteogenic differentiation in preosteoblasts via osteoimmune modulation.https://www.mdpi.com/2079-4991/13/11/1755mesoporous silica nanoparticlesmicroRNA-26aimmunomodulationosteogenesismacrophage |
| spellingShingle | Sepanta Hosseinpour Huan Dai Laurence J. Walsh Chun Xu Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro Nanomaterials mesoporous silica nanoparticles microRNA-26a immunomodulation osteogenesis macrophage |
| title | Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro |
| title_full | Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro |
| title_fullStr | Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro |
| title_full_unstemmed | Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro |
| title_short | Mesoporous Core–Cone Silica Nanoparticles Can Deliver miRNA-26a to Macrophages to Exert Immunomodulatory Effects on Osteogenesis In Vitro |
| title_sort | mesoporous core cone silica nanoparticles can deliver mirna 26a to macrophages to exert immunomodulatory effects on osteogenesis in vitro |
| topic | mesoporous silica nanoparticles microRNA-26a immunomodulation osteogenesis macrophage |
| url | https://www.mdpi.com/2079-4991/13/11/1755 |
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