Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among differe...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.685068/full |
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| author | Changrui Qian Changrui Qian Changrui Qian Hongmao Liu Hongmao Liu Hongmao Liu Jiawei Cao Yongan Ji Yongan Ji Wei Lu Wei Lu Junwan Lu Junwan Lu Aifang Li Xinyi Zhu Xinyi Zhu Xinyi Zhu Kai Shen Kai Shen Haili Xu Qianqian Chen Qianqian Chen Wangxiao Zhou Wangxiao Zhou Hongyun Lu Hailong Lin Hailong Lin Hailong Lin Xueya Zhang Xueya Zhang Xueya Zhang Qiaoling Li Qiaoling Li Qiaoling Li Xi Lin Xi Lin Kewei Li Kewei Li Teng Xu Teng Xu Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao Hailin Zhang Hailin Zhang |
| author_facet | Changrui Qian Changrui Qian Changrui Qian Hongmao Liu Hongmao Liu Hongmao Liu Jiawei Cao Yongan Ji Yongan Ji Wei Lu Wei Lu Junwan Lu Junwan Lu Aifang Li Xinyi Zhu Xinyi Zhu Xinyi Zhu Kai Shen Kai Shen Haili Xu Qianqian Chen Qianqian Chen Wangxiao Zhou Wangxiao Zhou Hongyun Lu Hailong Lin Hailong Lin Hailong Lin Xueya Zhang Xueya Zhang Xueya Zhang Qiaoling Li Qiaoling Li Qiaoling Li Xi Lin Xi Lin Kewei Li Kewei Li Teng Xu Teng Xu Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao Hailin Zhang Hailin Zhang |
| author_sort | Changrui Qian |
| collection | DOAJ |
| description | Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE. |
| first_indexed | 2024-12-16T13:14:43Z |
| format | Article |
| id | doaj.art-2f7d11e819ed432d88f41559de76a63d |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-16T13:14:43Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-2f7d11e819ed432d88f41559de76a63d2022-12-21T22:30:30ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-06-011110.3389/fcimb.2021.685068685068Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa IsolatesChangrui Qian0Changrui Qian1Changrui Qian2Hongmao Liu3Hongmao Liu4Hongmao Liu5Jiawei Cao6Yongan Ji7Yongan Ji8Wei Lu9Wei Lu10Junwan Lu11Junwan Lu12Aifang Li13Xinyi Zhu14Xinyi Zhu15Xinyi Zhu16Kai Shen17Kai Shen18Haili Xu19Qianqian Chen20Qianqian Chen21Wangxiao Zhou22Wangxiao Zhou23Hongyun Lu24Hailong Lin25Hailong Lin26Hailong Lin27Xueya Zhang28Xueya Zhang29Xueya Zhang30Qiaoling Li31Qiaoling Li32Qiaoling Li33Xi Lin34Xi Lin35Kewei Li36Kewei Li37Teng Xu38Teng Xu39Mei Zhu40Qiyu Bao41Qiyu Bao42Qiyu Bao43Hailin Zhang44Hailin Zhang45The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second People’s Hospital of Tongxiang City, Tongxiang, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Translational Medicine, Baotou Central Hospital, Baotou, ChinaTongji University School of Medicine, Shanghai, ChinaDepartment of Clinical Laboratory, Zhejiang Hospital, Hangzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaKey Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaThe Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, ChinaInstitute of Biomedical Informatics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, ChinaFlorfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE.https://www.frontiersin.org/articles/10.3389/fcimb.2021.685068/fullfloRflorfenicol resistancePseudomonas aeruginosaTn4371integrative and conjugative elements |
| spellingShingle | Changrui Qian Changrui Qian Changrui Qian Hongmao Liu Hongmao Liu Hongmao Liu Jiawei Cao Yongan Ji Yongan Ji Wei Lu Wei Lu Junwan Lu Junwan Lu Aifang Li Xinyi Zhu Xinyi Zhu Xinyi Zhu Kai Shen Kai Shen Haili Xu Qianqian Chen Qianqian Chen Wangxiao Zhou Wangxiao Zhou Hongyun Lu Hailong Lin Hailong Lin Hailong Lin Xueya Zhang Xueya Zhang Xueya Zhang Qiaoling Li Qiaoling Li Qiaoling Li Xi Lin Xi Lin Kewei Li Kewei Li Teng Xu Teng Xu Mei Zhu Qiyu Bao Qiyu Bao Qiyu Bao Hailin Zhang Hailin Zhang Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates Frontiers in Cellular and Infection Microbiology floR florfenicol resistance Pseudomonas aeruginosa Tn4371 integrative and conjugative elements |
| title | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
| title_full | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
| title_fullStr | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
| title_full_unstemmed | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
| title_short | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
| title_sort | identification of flor variants associated with a novel tn4371 like integrative and conjugative element in clinical pseudomonas aeruginosa isolates |
| topic | floR florfenicol resistance Pseudomonas aeruginosa Tn4371 integrative and conjugative elements |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.685068/full |
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