Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria

Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria

The RNA recognition motif (RRM) is the most common RNA-binding protein domain identified in nature. However, RRM-containing proteins are only prevalent in eukaryotic phyla, in which they play central regulatory roles. Here, we engineered an orthogonal post-transcriptional control system of gene expr...

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Bibliographic Details
Main Authors: Roswitha Dolcemascolo, María Heras-Hernández, Lucas Goiriz, Roser Montagud-Martínez, Alejandro Requena-Menéndez, Raúl Ruiz, Anna Pérez-Ràfols, R Anahí Higuera-Rodríguez, Guillermo Pérez-Ropero, Wim F Vranken, Tommaso Martelli, Wolfgang Kaiser, Jos Buijs, Guillermo Rodrigo
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2024-02-01
Series:eLife
Subjects:
binding kinetics
dynamic systems and modelling
genetic circuits
post-transcriptional regulation
RNA recognition motif
synthetic biology
Online Access:https://elifesciences.org/articles/91777

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