Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphis...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2017-05-01
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| Series: | The EuroBiotech Journal |
| Online Access: | https://doi.org/10.24190/ISSN2564-615X/2017/02.18 |
| _version_ | 1818730308431773696 |
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| author | Sysoliatin Eugeny Nikolaevich Anisimova Natalia Vladimirovna Babak Olga Gennadievna |
| author_facet | Sysoliatin Eugeny Nikolaevich Anisimova Natalia Vladimirovna Babak Olga Gennadievna |
| author_sort | Sysoliatin Eugeny Nikolaevich |
| collection | DOAJ |
| description | We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties. |
| first_indexed | 2024-12-17T22:59:43Z |
| format | Article |
| id | doaj.art-2f7f2fae56eb44649f17c96f60d6af8a |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-17T22:59:43Z |
| publishDate | 2017-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-2f7f2fae56eb44649f17c96f60d6af8a2022-12-21T21:29:26ZengSciendoThe EuroBiotech Journal2564-615X2017-05-011220020110.24190/ISSN2564-615X/2017/02.18Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primersSysoliatin Eugeny Nikolaevich0Anisimova Natalia Vladimirovna1Babak Olga Gennadievna2Laboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusLaboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusLaboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusWe examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.https://doi.org/10.24190/ISSN2564-615X/2017/02.18 |
| spellingShingle | Sysoliatin Eugeny Nikolaevich Anisimova Natalia Vladimirovna Babak Olga Gennadievna Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers The EuroBiotech Journal |
| title | Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers |
| title_full | Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers |
| title_fullStr | Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers |
| title_full_unstemmed | Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers |
| title_short | Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers |
| title_sort | genotyping lupinus angustifolius cultivars with srap molecular markers and degenerate primers |
| url | https://doi.org/10.24190/ISSN2564-615X/2017/02.18 |
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