Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field.
Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by relatively rapid wilting of maize plants before tasseling and until shortly before maturity. The disease's causal agent is the fungus Harpophora maydis, a soil-borne and seed-borne pathogen, which is cur...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0208353 |
| _version_ | 1818833648319725568 |
|---|---|
| author | Ofir Degani Shlomit Dor Daniel Movshowitz Eyal Fraidman Onn Rabinovitz Shaul Graph |
| author_facet | Ofir Degani Shlomit Dor Daniel Movshowitz Eyal Fraidman Onn Rabinovitz Shaul Graph |
| author_sort | Ofir Degani |
| collection | DOAJ |
| description | Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by relatively rapid wilting of maize plants before tasseling and until shortly before maturity. The disease's causal agent is the fungus Harpophora maydis, a soil-borne and seed-borne pathogen, which is currently controlled using reduced sensitivity maize cultivars. In a former study, we showed that Azoxystrobin (AS) injected into a drip irrigation line assigned for each row can suppress H. maydis in the field and that AS seed coating can provide an additional layer of protection. In the present study, we examine a more cost-effective protective treatment using this fungicide with Difenoconazole mixture (AS+DC), or Fluazinam, or Fluopyram and Trifloxystrobin mixture, or Prothioconazole and Tebuconazole mixture in combined treatment of seed coating and a drip irrigation line for two coupling rows. A recently developed Real-Time PCR method revealed that protecting the plants using AS+DC seed coating alone managed to delay pathogen DNA spread in the maize tissues, in the early stages of the growth season (up to the age of 50 days from sowing), but was less effective in protecting the crops later. AS+DC seed coating combined with drip irrigation using AS+DC was the most successful treatment, and in the double-row cultivation, it reduced fungal DNA in the host tissues to near zero levels. This treatment minimized the development of wilt symptoms by 41% and recovered cob yield by a factor of 1.6 (to the level common in healthy fields). Moreover, the yield classified as A class (cob weight of more than 250 g) increased from 58% to 75% in this treatment. This successful treatment against H. maydis in Israel can now be applied in vast areas to protect sensitive maize cultivars against maize late wilt disease. |
| first_indexed | 2024-12-19T02:22:16Z |
| format | Article |
| id | doaj.art-2f7f9d4252a34d8db462c8ac363a4fc9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:22:16Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2f7f9d4252a34d8db462c8ac363a4fc92022-12-21T20:40:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020835310.1371/journal.pone.0208353Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field.Ofir DeganiShlomit DorDaniel MovshowitzEyal FraidmanOnn RabinovitzShaul GraphLate wilt, a disease severely affecting maize fields throughout Israel, is characterized by relatively rapid wilting of maize plants before tasseling and until shortly before maturity. The disease's causal agent is the fungus Harpophora maydis, a soil-borne and seed-borne pathogen, which is currently controlled using reduced sensitivity maize cultivars. In a former study, we showed that Azoxystrobin (AS) injected into a drip irrigation line assigned for each row can suppress H. maydis in the field and that AS seed coating can provide an additional layer of protection. In the present study, we examine a more cost-effective protective treatment using this fungicide with Difenoconazole mixture (AS+DC), or Fluazinam, or Fluopyram and Trifloxystrobin mixture, or Prothioconazole and Tebuconazole mixture in combined treatment of seed coating and a drip irrigation line for two coupling rows. A recently developed Real-Time PCR method revealed that protecting the plants using AS+DC seed coating alone managed to delay pathogen DNA spread in the maize tissues, in the early stages of the growth season (up to the age of 50 days from sowing), but was less effective in protecting the crops later. AS+DC seed coating combined with drip irrigation using AS+DC was the most successful treatment, and in the double-row cultivation, it reduced fungal DNA in the host tissues to near zero levels. This treatment minimized the development of wilt symptoms by 41% and recovered cob yield by a factor of 1.6 (to the level common in healthy fields). Moreover, the yield classified as A class (cob weight of more than 250 g) increased from 58% to 75% in this treatment. This successful treatment against H. maydis in Israel can now be applied in vast areas to protect sensitive maize cultivars against maize late wilt disease.https://doi.org/10.1371/journal.pone.0208353 |
| spellingShingle | Ofir Degani Shlomit Dor Daniel Movshowitz Eyal Fraidman Onn Rabinovitz Shaul Graph Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. PLoS ONE |
| title | Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. |
| title_full | Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. |
| title_fullStr | Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. |
| title_full_unstemmed | Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. |
| title_short | Effective chemical protection against the maize late wilt causal agent, Harpophora maydis, in the field. |
| title_sort | effective chemical protection against the maize late wilt causal agent harpophora maydis in the field |
| url | https://doi.org/10.1371/journal.pone.0208353 |
| work_keys_str_mv | AT ofirdegani effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield AT shlomitdor effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield AT danielmovshowitz effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield AT eyalfraidman effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield AT onnrabinovitz effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield AT shaulgraph effectivechemicalprotectionagainstthemaizelatewiltcausalagentharpophoramaydisinthefield |