Characterizing diversity among human oral stem-like cancer cells using flow cytometry
Summary: We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006438 |
| _version_ | 1797980416741736448 |
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| author | Subhashis Ghosh Paromita Mitra Uday Saha Kavya Vipparthi Sandeep Singh |
| author_facet | Subhashis Ghosh Paromita Mitra Uday Saha Kavya Vipparthi Sandeep Singh |
| author_sort | Subhashis Ghosh |
| collection | DOAJ |
| description | Summary: We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines.For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T05:54:01Z |
| format | Article |
| id | doaj.art-2f7fb00f8c994dd8af1d14ad28346d1c |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T05:54:01Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2f7fb00f8c994dd8af1d14ad28346d1c2022-12-22T04:41:57ZengElsevierSTAR Protocols2666-16672022-12-0134101763Characterizing diversity among human oral stem-like cancer cells using flow cytometrySubhashis Ghosh0Paromita Mitra1Uday Saha2Kavya Vipparthi3Sandeep Singh4National Institute of Biomedical Genomics, Kalyani, West Bengal 741251, IndiaNational Institute of Biomedical Genomics, Kalyani, West Bengal 741251, IndiaNational Institute of Biomedical Genomics, Kalyani, West Bengal 741251, IndiaNational Institute of Biomedical Genomics, Kalyani, West Bengal 741251, IndiaNational Institute of Biomedical Genomics, Kalyani, West Bengal 741251, India; Corresponding authorSummary: We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines.For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006438Cell biologyCell cultureSingle cellFlow cytometry/Mass cytometryCell separation/fractionationCancer |
| spellingShingle | Subhashis Ghosh Paromita Mitra Uday Saha Kavya Vipparthi Sandeep Singh Characterizing diversity among human oral stem-like cancer cells using flow cytometry STAR Protocols Cell biology Cell culture Single cell Flow cytometry/Mass cytometry Cell separation/fractionation Cancer |
| title | Characterizing diversity among human oral stem-like cancer cells using flow cytometry |
| title_full | Characterizing diversity among human oral stem-like cancer cells using flow cytometry |
| title_fullStr | Characterizing diversity among human oral stem-like cancer cells using flow cytometry |
| title_full_unstemmed | Characterizing diversity among human oral stem-like cancer cells using flow cytometry |
| title_short | Characterizing diversity among human oral stem-like cancer cells using flow cytometry |
| title_sort | characterizing diversity among human oral stem like cancer cells using flow cytometry |
| topic | Cell biology Cell culture Single cell Flow cytometry/Mass cytometry Cell separation/fractionation Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006438 |
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