Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity
The aim of this study was to identify an anti-obesity peptide from <i>Allomyrina dichotoma</i> and investigate the lipid metabolic mechanism. Enzymatically hydrolyzed <i>A. dichotoma</i> larvae were further separated using tangential flow filtration and consecutive chromatogr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8537 |
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| author | Sung Mun Bae Meiqi Fan Young-Jin Choi Yujiao Tang Gwanghui Jeong Kyungjae Myung Byung-gyu Kim Eun-Kyung Kim |
| author_facet | Sung Mun Bae Meiqi Fan Young-Jin Choi Yujiao Tang Gwanghui Jeong Kyungjae Myung Byung-gyu Kim Eun-Kyung Kim |
| author_sort | Sung Mun Bae |
| collection | DOAJ |
| description | The aim of this study was to identify an anti-obesity peptide from <i>Allomyrina dichotoma</i> and investigate the lipid metabolic mechanism. Enzymatically hydrolyzed <i>A. dichotoma</i> larvae were further separated using tangential flow filtration and consecutive chromatographic processes. Finally, an anti-obesity peptide that showed the highest inhibitory effect on lipid accumulation was obtained, and the sequence was Glu-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 decreased lipid aggregation in vitro and significantly reduced the accumulation of body weight gain, liver weight, and adipose tissue weight in high-fat-fed mice. Compared with the control group, the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL), insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) in the high-fat diet (HFD) group increased significantly, and the content of high-density lipoprotein cholesterol (HDL) in the serum decreased significantly. On the contrary, the levels of TC, TG, and insulin in the EIA10 group decreased significantly, and the HDL content increased significantly compared with the HFD group. Additionally, EIA10 dramatically decreased mRNA and protein levels of transcription factors involved in lipid adipogenesis. Taken together, our results suggest that EIA10 could be a promising agent for the treatment and prevention of obesity. |
| first_indexed | 2024-03-10T14:53:41Z |
| format | Article |
| id | doaj.art-2f83c7e8fffd40779ff68804d2f9a6a4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:53:41Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2f83c7e8fffd40779ff68804d2f9a6a42023-11-20T20:45:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122853710.3390/ijms21228537Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in ObesitySung Mun Bae0Meiqi Fan1Young-Jin Choi2Yujiao Tang3Gwanghui Jeong4Kyungjae Myung5Byung-gyu Kim6Eun-Kyung Kim7Gyeongnam Agricultural Research and Extension Services, Jinju 52733, KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, KoreaGyeongnam Agricultural Research and Extension Services, Jinju 52733, KoreaCenter for Genomic Integrity, Institute for Basic Science, Ulsan 44919, KoreaCenter for Genomic Integrity, Institute for Basic Science, Ulsan 44919, KoreaDepartment of Food Science and Nutrition, Dong-A University, Busan 49315, KoreaThe aim of this study was to identify an anti-obesity peptide from <i>Allomyrina dichotoma</i> and investigate the lipid metabolic mechanism. Enzymatically hydrolyzed <i>A. dichotoma</i> larvae were further separated using tangential flow filtration and consecutive chromatographic processes. Finally, an anti-obesity peptide that showed the highest inhibitory effect on lipid accumulation was obtained, and the sequence was Glu-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 decreased lipid aggregation in vitro and significantly reduced the accumulation of body weight gain, liver weight, and adipose tissue weight in high-fat-fed mice. Compared with the control group, the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL), insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) in the high-fat diet (HFD) group increased significantly, and the content of high-density lipoprotein cholesterol (HDL) in the serum decreased significantly. On the contrary, the levels of TC, TG, and insulin in the EIA10 group decreased significantly, and the HDL content increased significantly compared with the HFD group. Additionally, EIA10 dramatically decreased mRNA and protein levels of transcription factors involved in lipid adipogenesis. Taken together, our results suggest that EIA10 could be a promising agent for the treatment and prevention of obesity.https://www.mdpi.com/1422-0067/21/22/8537<i>Allomyrina dichotoma</i> larvaepeptidelipid metabolismanti-obesity |
| spellingShingle | Sung Mun Bae Meiqi Fan Young-Jin Choi Yujiao Tang Gwanghui Jeong Kyungjae Myung Byung-gyu Kim Eun-Kyung Kim Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity International Journal of Molecular Sciences <i>Allomyrina dichotoma</i> larvae peptide lipid metabolism anti-obesity |
| title | Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity |
| title_full | Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity |
| title_fullStr | Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity |
| title_full_unstemmed | Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity |
| title_short | Exploring the Role of a Novel Peptide from <i>Allomyrina dichotoma</i> Larvae in Ameliorating Lipid Metabolism in Obesity |
| title_sort | exploring the role of a novel peptide from i allomyrina dichotoma i larvae in ameliorating lipid metabolism in obesity |
| topic | <i>Allomyrina dichotoma</i> larvae peptide lipid metabolism anti-obesity |
| url | https://www.mdpi.com/1422-0067/21/22/8537 |
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