Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry
Nuclear DNA content for the adult plants grown in a greenhouse and in vitro young plantlets of the pigeon orchid (Dendrobium crumenatum Sw.) was analyzed using flow cytometry. The resulting 2C DNA values ranged from 2.30±0.14 pgto 2.43±0.06 pg. However, nuclear DNA ploidy levels of long-term in vitr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2008-05-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjst/ejournal/journal/30-3/0125-3395-30-3-277-280.pdf |
| _version_ | 1811318052285841408 |
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| author | Upatham Meesawat Theera Srisawat Ladda Eksomtramage Kamnoon Kanchanapoom |
| author_facet | Upatham Meesawat Theera Srisawat Ladda Eksomtramage Kamnoon Kanchanapoom |
| author_sort | Upatham Meesawat |
| collection | DOAJ |
| description | Nuclear DNA content for the adult plants grown in a greenhouse and in vitro young plantlets of the pigeon orchid (Dendrobium crumenatum Sw.) was analyzed using flow cytometry. The resulting 2C DNA values ranged from 2.30±0.14 pgto 2.43±0.06 pg. However, nuclear DNA ploidy levels of long-term in vitro plantlets were found to be triploid and tetraploid.These ploidy levels were confirmed by chromosome counting. Tetraploid individuals (2n = 4x = 76) had approximately two times DNA content than diploid (2n = 2x = 38) individuals. This variation may be due to prolonged cultivation and thepresence of exogenous plant growth regulators. |
| first_indexed | 2024-04-13T12:20:17Z |
| format | Article |
| id | doaj.art-2f84288dc44248a5beaa4324a921a0b2 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-13T12:20:17Z |
| publishDate | 2008-05-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-2f84288dc44248a5beaa4324a921a0b22022-12-22T02:47:15ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952008-05-01303277280Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometryUpatham MeesawatTheera SrisawatLadda EksomtramageKamnoon KanchanapoomNuclear DNA content for the adult plants grown in a greenhouse and in vitro young plantlets of the pigeon orchid (Dendrobium crumenatum Sw.) was analyzed using flow cytometry. The resulting 2C DNA values ranged from 2.30±0.14 pgto 2.43±0.06 pg. However, nuclear DNA ploidy levels of long-term in vitro plantlets were found to be triploid and tetraploid.These ploidy levels were confirmed by chromosome counting. Tetraploid individuals (2n = 4x = 76) had approximately two times DNA content than diploid (2n = 2x = 38) individuals. This variation may be due to prolonged cultivation and thepresence of exogenous plant growth regulators.http://rdo.psu.ac.th/sjst/ejournal/journal/30-3/0125-3395-30-3-277-280.pdfDendrobium crumenatum Sw.DNA contentflow cytometryin vitro culturepigeon orchid |
| spellingShingle | Upatham Meesawat Theera Srisawat Ladda Eksomtramage Kamnoon Kanchanapoom Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry Songklanakarin Journal of Science and Technology (SJST) Dendrobium crumenatum Sw. DNA content flow cytometry in vitro culture pigeon orchid |
| title | Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry |
| title_full | Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry |
| title_fullStr | Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry |
| title_full_unstemmed | Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry |
| title_short | Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry |
| title_sort | nuclear dna content of the pigeon orchid dendrobium crumenatum sw with the analysis of flow cytometry |
| topic | Dendrobium crumenatum Sw. DNA content flow cytometry in vitro culture pigeon orchid |
| url | http://rdo.psu.ac.th/sjst/ejournal/journal/30-3/0125-3395-30-3-277-280.pdf |
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