Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS
Abstract Objective The present study aimed to determine the effect and mechanical mechanism of spontaneous breathing during mechanical ventilation on oxygenation and lung injury using Beagles dogs mild or moderate acute respiratory distress syndrome (ARDS) model. Methods After inducing mild or moder...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Pulmonary Medicine |
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| Online Access: | https://doi.org/10.1186/s12890-023-02730-y |
| _version_ | 1797637094002130944 |
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| author | Rui Yang Leilei Zhou Zongyu Chen Shuang He Siyu Lian Yi Shen Xianming Zhang |
| author_facet | Rui Yang Leilei Zhou Zongyu Chen Shuang He Siyu Lian Yi Shen Xianming Zhang |
| author_sort | Rui Yang |
| collection | DOAJ |
| description | Abstract Objective The present study aimed to determine the effect and mechanical mechanism of spontaneous breathing during mechanical ventilation on oxygenation and lung injury using Beagles dogs mild or moderate acute respiratory distress syndrome (ARDS) model. Methods After inducing mild or moderate ARDS by infusion of oleic acid, Eighteen Beagles dogs were randomly split into Spontaneous breathing group (BIPAPSB, n = 6), and Complete muscle paralysis group (BIPAPPC, n = 6),Six Beagles without ventilator support comprised the control group. Both groups were ventilated for 8 h under BIPAP mode. High-pressure was titrated TV to 6 ml/kg. A multi-pair esophageal balloon electrode catheter was used to measure respiratory mechanics and electromyogram. End-expiratory lung volume (EELV), gas exchange and respiratory variables were recorded in the process of mechanical ventilation. The contents of Interleukin (IL)-6 and IL-8 in lung tissue were measure using qRT-PCR. Besides, lung injury score was calculated in the end of mechanical ventilation. Results Based on the comparable setting of ventilator, BIPAPSB group exhibited higher safety peak transpulmonary pressure, abdominal pressure, EELV and P/F(PaO2/FiO2) than BIPAPPC group, whereas mean transpulmonary pressure, the mRNA levels of the IL-6 and IL-8 in the lung tissues and lung injury score in BIPAPSB group were lower than those in BIPAPPC group. Conclusion In mild to moderate ARDS animal models, during mechanical ventilation, SB may improve respiratory function and reduce ventilator-induced lung injury. The mechanism may be that spontaneous inspiration up-regulates peak transpulmonary pressure and EELV; Spontaneous expiration decreases mean transpulmonary pressure by up-regulating intra-abdominal pressure, thereby reducing stress and strain. |
| first_indexed | 2024-03-11T12:44:27Z |
| format | Article |
| id | doaj.art-2f84a72b1d774b83b03ab0f7e21c56e8 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-03-11T12:44:27Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-2f84a72b1d774b83b03ab0f7e21c56e82023-11-05T12:06:27ZengBMCBMC Pulmonary Medicine1471-24662023-11-012311910.1186/s12890-023-02730-yEffect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDSRui Yang0Leilei Zhou1Zongyu Chen2Shuang He3Siyu Lian4Yi Shen5Xianming Zhang6First People’s Hospital of Guiyang CityDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalDepartment of Respiratory Medicine, The Affiliated Hospital of Guizhou MedicalAbstract Objective The present study aimed to determine the effect and mechanical mechanism of spontaneous breathing during mechanical ventilation on oxygenation and lung injury using Beagles dogs mild or moderate acute respiratory distress syndrome (ARDS) model. Methods After inducing mild or moderate ARDS by infusion of oleic acid, Eighteen Beagles dogs were randomly split into Spontaneous breathing group (BIPAPSB, n = 6), and Complete muscle paralysis group (BIPAPPC, n = 6),Six Beagles without ventilator support comprised the control group. Both groups were ventilated for 8 h under BIPAP mode. High-pressure was titrated TV to 6 ml/kg. A multi-pair esophageal balloon electrode catheter was used to measure respiratory mechanics and electromyogram. End-expiratory lung volume (EELV), gas exchange and respiratory variables were recorded in the process of mechanical ventilation. The contents of Interleukin (IL)-6 and IL-8 in lung tissue were measure using qRT-PCR. Besides, lung injury score was calculated in the end of mechanical ventilation. Results Based on the comparable setting of ventilator, BIPAPSB group exhibited higher safety peak transpulmonary pressure, abdominal pressure, EELV and P/F(PaO2/FiO2) than BIPAPPC group, whereas mean transpulmonary pressure, the mRNA levels of the IL-6 and IL-8 in the lung tissues and lung injury score in BIPAPSB group were lower than those in BIPAPPC group. Conclusion In mild to moderate ARDS animal models, during mechanical ventilation, SB may improve respiratory function and reduce ventilator-induced lung injury. The mechanism may be that spontaneous inspiration up-regulates peak transpulmonary pressure and EELV; Spontaneous expiration decreases mean transpulmonary pressure by up-regulating intra-abdominal pressure, thereby reducing stress and strain.https://doi.org/10.1186/s12890-023-02730-yAcute Respiratory Distress Syndrome (ARDS)Spontaneous Breathing (SB)Complete muscle paralysis(PC)Beagle dogsEnd-Expiratory Lung Volume (EELV) |
| spellingShingle | Rui Yang Leilei Zhou Zongyu Chen Shuang He Siyu Lian Yi Shen Xianming Zhang Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS BMC Pulmonary Medicine Acute Respiratory Distress Syndrome (ARDS) Spontaneous Breathing (SB) Complete muscle paralysis(PC) Beagle dogs End-Expiratory Lung Volume (EELV) |
| title | Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS |
| title_full | Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS |
| title_fullStr | Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS |
| title_full_unstemmed | Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS |
| title_short | Effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ARDS |
| title_sort | effect and mechanical mechanism of spontaneous breathing on oxygenation and lung injury in mild or moderate animal ards |
| topic | Acute Respiratory Distress Syndrome (ARDS) Spontaneous Breathing (SB) Complete muscle paralysis(PC) Beagle dogs End-Expiratory Lung Volume (EELV) |
| url | https://doi.org/10.1186/s12890-023-02730-y |
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