The transcriptional response of Arcobacter butzleri to cold shock
Arcobacter (A.) butzleri is an emerging zoonotic pathogen associated with gastrointestinal diseases, such as abdominal cramps and diarrhea, and is widely detected in animals, showing a high prevalence in poultry and seafood. The survival and adaptation of A. butzleri to cold temperatures remains poo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-10-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12959 |
| _version_ | 1818541560915034112 |
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| author | Xiaochen Zhang Yulan Su Thomas Alter Greta Gölz |
| author_facet | Xiaochen Zhang Yulan Su Thomas Alter Greta Gölz |
| author_sort | Xiaochen Zhang |
| collection | DOAJ |
| description | Arcobacter (A.) butzleri is an emerging zoonotic pathogen associated with gastrointestinal diseases, such as abdominal cramps and diarrhea, and is widely detected in animals, showing a high prevalence in poultry and seafood. The survival and adaptation of A. butzleri to cold temperatures remains poorly studied, although it might be of interest for food safety considerations. To address this, growth patterns of eight A. butzleri isolates were determined at 8 °C for 28 days. A. butzleri isolates showed strain‐dependent behavior: six isolates were unculturable after day 18, one exhibited declining but detectable cell counts until day 28 and one grew to the stationary phase level. Out of 13 A. butzleri cold shock‐related genes homologous to Escherichia coli, 10 were up‐regulated in response to a temperature downshift to 8 °C, as demonstrated by reverse transcription‐quantitative PCR. Additionally, we compared these data with the cold‐shock response in E. coli. Overall, we provide a deeper insight into the environmental adaptation capacities of A. butzleri, which we find shares similarities with the E. coli cold‐shock response. |
| first_indexed | 2024-12-11T22:10:53Z |
| format | Article |
| id | doaj.art-2f88b3d3bbbb45efb0cdbbb714dc615a |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-11T22:10:53Z |
| publishDate | 2020-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-2f88b3d3bbbb45efb0cdbbb714dc615a2022-12-22T00:48:46ZengWileyFEBS Open Bio2211-54632020-10-0110102089209610.1002/2211-5463.12959The transcriptional response of Arcobacter butzleri to cold shockXiaochen Zhang0Yulan Su1Thomas Alter2Greta Gölz3Institute of Food Safety and Food Hygiene Freie Universität Berlin Berlin GermanyInstitute of Food Safety and Food Hygiene Freie Universität Berlin Berlin GermanyInstitute of Food Safety and Food Hygiene Freie Universität Berlin Berlin GermanyInstitute of Food Safety and Food Hygiene Freie Universität Berlin Berlin GermanyArcobacter (A.) butzleri is an emerging zoonotic pathogen associated with gastrointestinal diseases, such as abdominal cramps and diarrhea, and is widely detected in animals, showing a high prevalence in poultry and seafood. The survival and adaptation of A. butzleri to cold temperatures remains poorly studied, although it might be of interest for food safety considerations. To address this, growth patterns of eight A. butzleri isolates were determined at 8 °C for 28 days. A. butzleri isolates showed strain‐dependent behavior: six isolates were unculturable after day 18, one exhibited declining but detectable cell counts until day 28 and one grew to the stationary phase level. Out of 13 A. butzleri cold shock‐related genes homologous to Escherichia coli, 10 were up‐regulated in response to a temperature downshift to 8 °C, as demonstrated by reverse transcription‐quantitative PCR. Additionally, we compared these data with the cold‐shock response in E. coli. Overall, we provide a deeper insight into the environmental adaptation capacities of A. butzleri, which we find shares similarities with the E. coli cold‐shock response.https://doi.org/10.1002/2211-5463.12959A. butzlericold adaptationcold shock‐related genestemporal transcriptional expression |
| spellingShingle | Xiaochen Zhang Yulan Su Thomas Alter Greta Gölz The transcriptional response of Arcobacter butzleri to cold shock FEBS Open Bio A. butzleri cold adaptation cold shock‐related genes temporal transcriptional expression |
| title | The transcriptional response of Arcobacter butzleri to cold shock |
| title_full | The transcriptional response of Arcobacter butzleri to cold shock |
| title_fullStr | The transcriptional response of Arcobacter butzleri to cold shock |
| title_full_unstemmed | The transcriptional response of Arcobacter butzleri to cold shock |
| title_short | The transcriptional response of Arcobacter butzleri to cold shock |
| title_sort | transcriptional response of arcobacter butzleri to cold shock |
| topic | A. butzleri cold adaptation cold shock‐related genes temporal transcriptional expression |
| url | https://doi.org/10.1002/2211-5463.12959 |
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