Cultured circulating tumor cells and their derived xenografts for personalized oncology
Recent cancer research has demonstrated the existence of circulating tumor cells (CTCs) in cancer patient's blood. Once identified, CTC biomarkers will be invaluable tools for clinical diagnosis, prognosis and treatment. In this review, we propose ex vivo culture as a rational strategy for larg...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Asian Journal of Urology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214388216300492 |
| _version_ | 1828398448058040320 |
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| author | Ruoxiang Wang Gina C.Y. Chu Stefan Mrdenovic Alagappan A. Annamalai Andrew E. Hendifar Nicholas N. Nissen James S. Tomlinson Michael Lewis Nallasivam Palanisamy Hsian-Rong Tseng Edwin M. Posadas Michael R. Freeman Stephen J. Pandol Haiyen E. Zhau Leland W.K. Chung |
| author_facet | Ruoxiang Wang Gina C.Y. Chu Stefan Mrdenovic Alagappan A. Annamalai Andrew E. Hendifar Nicholas N. Nissen James S. Tomlinson Michael Lewis Nallasivam Palanisamy Hsian-Rong Tseng Edwin M. Posadas Michael R. Freeman Stephen J. Pandol Haiyen E. Zhau Leland W.K. Chung |
| author_sort | Ruoxiang Wang |
| collection | DOAJ |
| description | Recent cancer research has demonstrated the existence of circulating tumor cells (CTCs) in cancer patient's blood. Once identified, CTC biomarkers will be invaluable tools for clinical diagnosis, prognosis and treatment. In this review, we propose ex vivo culture as a rational strategy for large scale amplification of the limited numbers of CTCs from a patient sample, to derive enough CTCs for accurate and reproducible characterization of the biophysical, biochemical, gene expressional and behavioral properties of the harvested cells. Because of tumor cell heterogeneity, it is important to amplify all the CTCs in a blood sample for a comprehensive understanding of their role in cancer metastasis. By analyzing critical steps and technical issues in ex vivo CTC culture, we developed a cost-effective and reproducible protocol directly culturing whole peripheral blood mononuclear cells, relying on an assumed survival advantage in CTCs and CTC-like cells over the normal cells to amplify this specified cluster of cancer cells. Keywords: Cancer metastasis, Peripheral blood, Circulating tumor cell, ex vivo culture |
| first_indexed | 2024-12-10T09:01:38Z |
| format | Article |
| id | doaj.art-2f8af182edee419dbf7dc3e0c70b182a |
| institution | Directory Open Access Journal |
| issn | 2214-3882 |
| language | English |
| last_indexed | 2024-12-10T09:01:38Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Urology |
| spelling | doaj.art-2f8af182edee419dbf7dc3e0c70b182a2022-12-22T01:55:16ZengElsevierAsian Journal of Urology2214-38822016-10-0134240253Cultured circulating tumor cells and their derived xenografts for personalized oncologyRuoxiang Wang0Gina C.Y. Chu1Stefan Mrdenovic2Alagappan A. Annamalai3Andrew E. Hendifar4Nicholas N. Nissen5James S. Tomlinson6Michael Lewis7Nallasivam Palanisamy8Hsian-Rong Tseng9Edwin M. Posadas10Michael R. Freeman11Stephen J. Pandol12Haiyen E. Zhau13Leland W.K. Chung14Uro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Corresponding author.Uro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Surgery, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Surgery, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USADepartment of Surgery, West Los Angeles VA Hospital, Greater Los Angeles Veterans Affairs Healthcare System, Los Angeles, CA, USADepartment of Pathology, West Los Angeles VA Hospital, Greater Los Angeles Veterans Affairs Healthcare System, Los Angeles, CA, USAHenry Ford Health System, Detroit, MI, USADepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Surgery, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USAUro-Oncology Research, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA; Uro-Oncology Research, Department of Surgery, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USARecent cancer research has demonstrated the existence of circulating tumor cells (CTCs) in cancer patient's blood. Once identified, CTC biomarkers will be invaluable tools for clinical diagnosis, prognosis and treatment. In this review, we propose ex vivo culture as a rational strategy for large scale amplification of the limited numbers of CTCs from a patient sample, to derive enough CTCs for accurate and reproducible characterization of the biophysical, biochemical, gene expressional and behavioral properties of the harvested cells. Because of tumor cell heterogeneity, it is important to amplify all the CTCs in a blood sample for a comprehensive understanding of their role in cancer metastasis. By analyzing critical steps and technical issues in ex vivo CTC culture, we developed a cost-effective and reproducible protocol directly culturing whole peripheral blood mononuclear cells, relying on an assumed survival advantage in CTCs and CTC-like cells over the normal cells to amplify this specified cluster of cancer cells. Keywords: Cancer metastasis, Peripheral blood, Circulating tumor cell, ex vivo culturehttp://www.sciencedirect.com/science/article/pii/S2214388216300492 |
| spellingShingle | Ruoxiang Wang Gina C.Y. Chu Stefan Mrdenovic Alagappan A. Annamalai Andrew E. Hendifar Nicholas N. Nissen James S. Tomlinson Michael Lewis Nallasivam Palanisamy Hsian-Rong Tseng Edwin M. Posadas Michael R. Freeman Stephen J. Pandol Haiyen E. Zhau Leland W.K. Chung Cultured circulating tumor cells and their derived xenografts for personalized oncology Asian Journal of Urology |
| title | Cultured circulating tumor cells and their derived xenografts for personalized oncology |
| title_full | Cultured circulating tumor cells and their derived xenografts for personalized oncology |
| title_fullStr | Cultured circulating tumor cells and their derived xenografts for personalized oncology |
| title_full_unstemmed | Cultured circulating tumor cells and their derived xenografts for personalized oncology |
| title_short | Cultured circulating tumor cells and their derived xenografts for personalized oncology |
| title_sort | cultured circulating tumor cells and their derived xenografts for personalized oncology |
| url | http://www.sciencedirect.com/science/article/pii/S2214388216300492 |
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