Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages
IntroductionThe lncRNAs (long non-coding RNAs) are the most diverse group of non-coding RNAs and are involved in most biological processes including the immune response. While some of them have been recognized for their influence on the regulation of inflammatory activity, little is known in the con...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1352306/full |
| _version_ | 1797299345519804416 |
|---|---|
| author | Manuel Flores-Concha Leonardo A. Gómez Rodrigo Soto-Shara Raúl E. Molina Roberto F. Coloma-Rivero David A. Montero Ítalo Ferrari Ángel Oñate |
| author_facet | Manuel Flores-Concha Leonardo A. Gómez Rodrigo Soto-Shara Raúl E. Molina Roberto F. Coloma-Rivero David A. Montero Ítalo Ferrari Ángel Oñate |
| author_sort | Manuel Flores-Concha |
| collection | DOAJ |
| description | IntroductionThe lncRNAs (long non-coding RNAs) are the most diverse group of non-coding RNAs and are involved in most biological processes including the immune response. While some of them have been recognized for their influence on the regulation of inflammatory activity, little is known in the context of infection by Brucella abortus, a pathogen that presents significant challenges due to its ability to manipulate and evade the host immune system. This study focuses on characterize the expression profile of LincRNA-cox2, Lethe, lincRNA-EPS, Malat1 and Gas5 during infection of macrophages by B. abortus.MethodsUsing public raw RNA-seq datasets we constructed for a lncRNA expression profile in macrophages Brucella-infected. In addition, from public RNA-seq raw datasets of RAW264.7 cells infected with B. abortus we constructed a transcriptomic profile of lncRNAs in order to know the expression of the five immunomodulating lncRNAs studied here at 8 and 24 h post-infection. Finally, we performed in vitro infection assays in RAW264.7 cells and peritoneal macrophages to detect by qPCR changes in the expression of these lncRNAs at first 12 hours post infection, a key stage in the infection cycle where Brucella modulates the immune response to survive.ResultsOur results demonstrate that infection of macrophages with Brucella abortus, induces significant changes in the expression of LincRNA-Cox2, Lethe, LincRNA-EPS, Gas5, and Malat1.DiscussionThe change in the expression profile of these immunomodulatory lncRNAs in response to infection, suggest a potential involvement in the immune evasion strategy employed by Brucella to facilitate its intracellular survival. |
| first_indexed | 2024-03-07T22:49:15Z |
| format | Article |
| id | doaj.art-2f8b344a912e42a58bbd30f787bdeac0 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-07T22:49:15Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2f8b344a912e42a58bbd30f787bdeac02024-02-23T10:43:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-02-011510.3389/fimmu.2024.13523061352306Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophagesManuel Flores-ConchaLeonardo A. GómezRodrigo Soto-SharaRaúl E. MolinaRoberto F. Coloma-RiveroDavid A. MonteroÍtalo FerrariÁngel OñateIntroductionThe lncRNAs (long non-coding RNAs) are the most diverse group of non-coding RNAs and are involved in most biological processes including the immune response. While some of them have been recognized for their influence on the regulation of inflammatory activity, little is known in the context of infection by Brucella abortus, a pathogen that presents significant challenges due to its ability to manipulate and evade the host immune system. This study focuses on characterize the expression profile of LincRNA-cox2, Lethe, lincRNA-EPS, Malat1 and Gas5 during infection of macrophages by B. abortus.MethodsUsing public raw RNA-seq datasets we constructed for a lncRNA expression profile in macrophages Brucella-infected. In addition, from public RNA-seq raw datasets of RAW264.7 cells infected with B. abortus we constructed a transcriptomic profile of lncRNAs in order to know the expression of the five immunomodulating lncRNAs studied here at 8 and 24 h post-infection. Finally, we performed in vitro infection assays in RAW264.7 cells and peritoneal macrophages to detect by qPCR changes in the expression of these lncRNAs at first 12 hours post infection, a key stage in the infection cycle where Brucella modulates the immune response to survive.ResultsOur results demonstrate that infection of macrophages with Brucella abortus, induces significant changes in the expression of LincRNA-Cox2, Lethe, LincRNA-EPS, Gas5, and Malat1.DiscussionThe change in the expression profile of these immunomodulatory lncRNAs in response to infection, suggest a potential involvement in the immune evasion strategy employed by Brucella to facilitate its intracellular survival.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1352306/fullbrucella abortusmacrophages infectiongene expressionlncRNAimmunomodulation |
| spellingShingle | Manuel Flores-Concha Leonardo A. Gómez Rodrigo Soto-Shara Raúl E. Molina Roberto F. Coloma-Rivero David A. Montero Ítalo Ferrari Ángel Oñate Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages Frontiers in Immunology brucella abortus macrophages infection gene expression lncRNA immunomodulation |
| title | Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages |
| title_full | Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages |
| title_fullStr | Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages |
| title_full_unstemmed | Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages |
| title_short | Brucella abortus triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages |
| title_sort | brucella abortus triggers the differential expression of immunomodulatory lncrnas in infected murine macrophages |
| topic | brucella abortus macrophages infection gene expression lncRNA immunomodulation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1352306/full |
| work_keys_str_mv | AT manuelfloresconcha brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT leonardoagomez brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT rodrigosotoshara brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT raulemolina brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT robertofcolomarivero brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT davidamontero brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT italoferrari brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages AT angelonate brucellaabortustriggersthedifferentialexpressionofimmunomodulatorylncrnasininfectedmurinemacrophages |