Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats
Purpose: The aim of this study is to investigate the anti-inflammatory and anti-apoptotic effects of naringin (NRG), which has many biological properties, on bacterial endotoxin-induced small intestine damage in rats. Materials and Methods: For this purpose, 40 female Wistar albino rats were divided...
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| Format: | Article |
| Language: | English |
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Cukurova University
2022-09-01
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| Series: | Cukurova Medical Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2460970 |
| _version_ | 1828017141240037376 |
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| author | Ayşe Toluk Derya Karabulut Necla Değer Meryem Sayan Tayfun Ceylan Emin Kaymak Mohamed Lemine El Bechir Ali Tuğrul Akin |
| author_facet | Ayşe Toluk Derya Karabulut Necla Değer Meryem Sayan Tayfun Ceylan Emin Kaymak Mohamed Lemine El Bechir Ali Tuğrul Akin |
| author_sort | Ayşe Toluk |
| collection | DOAJ |
| description | Purpose: The aim of this study is to investigate the anti-inflammatory and anti-apoptotic effects of naringin (NRG), which has many biological properties, on bacterial endotoxin-induced small intestine damage in rats.
Materials and Methods: For this purpose, 40 female Wistar albino rats were divided into 4 groups as Control (group given no treatment), LPS (group given 10 mg/kg/i.p lipopolysaccharide), NRG (group given 100 mg/kg/i.p naringin for 14 days) and LPS + NRG (group given 100 mg/kg/i.p naringin for 14 days before 10 mg/kg/i.p lipopolysaccharide injection). After experimental procedure, small intestine tissues of animals were extracted and prepared according to tissue processing protocol. Hematoxylin and Eosin staining were performed to evaluate the histopathological changes and histological damage scoring was applied to compare experimental groups in terms of histopathological changes. Moreover, TNF- and Caspase-3 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were scored to determine the difference in the expression levels of TNF- and Caspase-3 expressions among groups.
Results: Epithelial and Brunner’s gland damage, mononuclear cell infiltration, hemorrhage, and TNF- and Caspase-3 expressions significantly increased in the LPS group. However, NRG administrations exerted a strong protective effect on the small intestine tissues in terms of these parameters in LPS+NRG group.
Conclusion: This study demonstrated that 100 mg/kg NRG injection can be regarded as a protective agent against negative effects of endotoxin-induced infection on the intestinal mucosa and that it should not be disregarded in further clinical trials. |
| first_indexed | 2024-04-10T10:45:10Z |
| format | Article |
| id | doaj.art-2f8be556e48a4ea69288fd4346222252 |
| institution | Directory Open Access Journal |
| issn | 2602-3040 |
| language | English |
| last_indexed | 2024-04-10T10:45:10Z |
| publishDate | 2022-09-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Cukurova Medical Journal |
| spelling | doaj.art-2f8be556e48a4ea69288fd43462222522023-02-15T16:20:21ZengCukurova UniversityCukurova Medical Journal2602-30402022-09-014731137114610.17826/cumj.112464148Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in ratsAyşe Toluk0Derya Karabulut1Necla Değer2Meryem Sayan3Tayfun Ceylan4Emin Kaymak5Mohamed Lemine El Bechir6Ali Tuğrul Akin7ERCİYES ÜNİVERSİTESİERCIYES UNIVERSITYERCİYES ÜNİVERSİTESİERCİYES ÜNİVERSİTESİKAPADOKYA MESLEK YÜKSEKOKULU, KAPADOKYA MESLEK YÜKSEKOKULUBOZOK UNIVERSITYERCİYES ÜNİVERSİTESİERCIYES UNIVERSITYPurpose: The aim of this study is to investigate the anti-inflammatory and anti-apoptotic effects of naringin (NRG), which has many biological properties, on bacterial endotoxin-induced small intestine damage in rats. Materials and Methods: For this purpose, 40 female Wistar albino rats were divided into 4 groups as Control (group given no treatment), LPS (group given 10 mg/kg/i.p lipopolysaccharide), NRG (group given 100 mg/kg/i.p naringin for 14 days) and LPS + NRG (group given 100 mg/kg/i.p naringin for 14 days before 10 mg/kg/i.p lipopolysaccharide injection). After experimental procedure, small intestine tissues of animals were extracted and prepared according to tissue processing protocol. Hematoxylin and Eosin staining were performed to evaluate the histopathological changes and histological damage scoring was applied to compare experimental groups in terms of histopathological changes. Moreover, TNF- and Caspase-3 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were scored to determine the difference in the expression levels of TNF- and Caspase-3 expressions among groups. Results: Epithelial and Brunner’s gland damage, mononuclear cell infiltration, hemorrhage, and TNF- and Caspase-3 expressions significantly increased in the LPS group. However, NRG administrations exerted a strong protective effect on the small intestine tissues in terms of these parameters in LPS+NRG group. Conclusion: This study demonstrated that 100 mg/kg NRG injection can be regarded as a protective agent against negative effects of endotoxin-induced infection on the intestinal mucosa and that it should not be disregarded in further clinical trials.https://dergipark.org.tr/tr/download/article-file/2460970endotoksini̇nce bağırsak hasarılipopolisakkaritnaringinendotoxinsmall intestine damagelipopolysaccharidenaringin |
| spellingShingle | Ayşe Toluk Derya Karabulut Necla Değer Meryem Sayan Tayfun Ceylan Emin Kaymak Mohamed Lemine El Bechir Ali Tuğrul Akin Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats Cukurova Medical Journal endotoksin i̇nce bağırsak hasarı lipopolisakkarit naringin endotoxin small intestine damage lipopolysaccharide naringin |
| title | Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats |
| title_full | Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats |
| title_fullStr | Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats |
| title_full_unstemmed | Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats |
| title_short | Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats |
| title_sort | anti inflammatory and anti apoptotic effects of naringin on bacterial endotoxin induced small intestine damage in rats |
| topic | endotoksin i̇nce bağırsak hasarı lipopolisakkarit naringin endotoxin small intestine damage lipopolysaccharide naringin |
| url | https://dergipark.org.tr/tr/download/article-file/2460970 |
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