Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615
Introduction: Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics. PHB synthase encoded by phbC gene is the main enzyme in PHB biosynthesis pathway in Ralstonia eutropha. The aim of current s...
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| Format: | Article |
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Tabriz University of Medical Sciences
2021-01-01
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| Series: | BioImpacts |
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| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-11-53.pdf |
| _version_ | 1819102226350604288 |
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| author | Farzaneh Barati Ezat Asgarani Sara Gharavi Mohammad Reza Soudi |
| author_facet | Farzaneh Barati Ezat Asgarani Sara Gharavi Mohammad Reza Soudi |
| author_sort | Farzaneh Barati |
| collection | DOAJ |
| description | Introduction: Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics. PHB synthase encoded by phbC gene is the main enzyme in PHB biosynthesis pathway in Ralstonia eutropha. The aim of current study was the transformation of R. eutropha PTCC 1615 with its own phbC gene and evaluation of the overexpression effect on PHB accumulation. Methods: DNA fragment including phbC gene and its promoter and terminator regions, was isolated from R. eutropha PTCC 1615, inserted into pET28a(+) vector, and transferred to the competent bacteria using calcium chloride and heat shock method. The effect of the cloned gene expression on PHB production was investigated with absorption of crotonic acid produced through PHB dehydration. Statistical analyses were carried out by SPSS software. Results: PHB content of cells of the engineered strain was 1.4 times more than that of the native bacteria. This significant difference can be an important finding for improvement of biopolymer production. Conclusion: Overexpression of phbC, the critical gene in PHB biosynthesis pathway, in R. eutropha PTCC 1615 had considerable effect on PHB accumulation. |
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| id | doaj.art-2f90576f8f974810b46f3b219ca7fef0 |
| institution | Directory Open Access Journal |
| issn | 2228-5660 2228-5652 |
| language | English |
| last_indexed | 2024-12-22T01:31:12Z |
| publishDate | 2021-01-01 |
| publisher | Tabriz University of Medical Sciences |
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| series | BioImpacts |
| spelling | doaj.art-2f90576f8f974810b46f3b219ca7fef02022-12-21T18:43:29ZengTabriz University of Medical SciencesBioImpacts2228-56602228-56522021-01-01111535710.34172/bi.2021.07bi-21880Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615Farzaneh Barati0Ezat Asgarani1Sara Gharavi2Mohammad Reza Soudi3Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, IranDepartment of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, IranDepartment of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran2 Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, IranIntroduction: Poly(3-hydroxybutyrate) (PHB) is a well-known biodegradable polymer produced by some microorganisms and can be a suitable alternative for petrochemical plastics. PHB synthase encoded by phbC gene is the main enzyme in PHB biosynthesis pathway in Ralstonia eutropha. The aim of current study was the transformation of R. eutropha PTCC 1615 with its own phbC gene and evaluation of the overexpression effect on PHB accumulation. Methods: DNA fragment including phbC gene and its promoter and terminator regions, was isolated from R. eutropha PTCC 1615, inserted into pET28a(+) vector, and transferred to the competent bacteria using calcium chloride and heat shock method. The effect of the cloned gene expression on PHB production was investigated with absorption of crotonic acid produced through PHB dehydration. Statistical analyses were carried out by SPSS software. Results: PHB content of cells of the engineered strain was 1.4 times more than that of the native bacteria. This significant difference can be an important finding for improvement of biopolymer production. Conclusion: Overexpression of phbC, the critical gene in PHB biosynthesis pathway, in R. eutropha PTCC 1615 had considerable effect on PHB accumulation.https://bi.tbzmed.ac.ir/PDF/bi-11-53.pdfralstonia eutrophapoly(3-hydroxybutyrate)phb synthasetransformation |
| spellingShingle | Farzaneh Barati Ezat Asgarani Sara Gharavi Mohammad Reza Soudi Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 BioImpacts ralstonia eutropha poly(3-hydroxybutyrate) phb synthase transformation |
| title | Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 |
| title_full | Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 |
| title_fullStr | Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 |
| title_full_unstemmed | Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 |
| title_short | Considerable increase in Poly(3-hydroxybutyrate) production via phbC gene overexpression in Ralstonia eutropha PTCC 1615 |
| title_sort | considerable increase in poly 3 hydroxybutyrate production via phbc gene overexpression in ralstonia eutropha ptcc 1615 |
| topic | ralstonia eutropha poly(3-hydroxybutyrate) phb synthase transformation |
| url | https://bi.tbzmed.ac.ir/PDF/bi-11-53.pdf |
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