Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
CRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs (crRNAs). To improve the efficiency of the CRISPR-Cas12a system for multiplex genome editing,...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2020-06-01
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Series: | Crop Journal |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S221451411930090X |