Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D
Synthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140–158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21–35)], has been...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00498/full |
| _version_ | 1818498263365451776 |
|---|---|
| author | Rodrigo Cañas-Arranz Patricia de León Mar Forner Sira Defaus María J. Bustos Elisa Torres David Andreu Esther Blanco Francisco Sobrino |
| author_facet | Rodrigo Cañas-Arranz Patricia de León Mar Forner Sira Defaus María J. Bustos Elisa Torres David Andreu Esther Blanco Francisco Sobrino |
| author_sort | Rodrigo Cañas-Arranz |
| collection | DOAJ |
| description | Synthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140–158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21–35)], has been shown to protect pigs against viral challenge. Interestingly, the modular design of this dendrimer peptide allows modifications aimed at improving its immunogenicity, such as the replacement of the T-cell epitope moiety. Here, we report that a dendrimer peptide, B2T-3D, harboring a T-cell epitope from FMDV 3D protein [3D (56–70)], when inoculated in pigs, elicited consistent levels of neutralizing antibodies and high frequencies of IFN-γ-producing cells upon in vitro recall with the homologous dendrimers, both responses being similar to those evoked by B2T-3A. Lymphocytes from B2T-3A-immunized pigs were in vitro-stimulated by T-3A peptide and to a lesser extent by B-peptide, while those from B2T-3D- immunized animals preferentially recognized the T-3D peptide, suggesting that this epitope is a potent inducer of IFN-γ producing-cells. These results extend the repertoire of T-cell epitopes efficiently recognized by swine lymphocytes and open the possibility of using T-3D to enhance the immunogenicity and the protection conferred by B2T-dendrimers. |
| first_indexed | 2024-12-10T18:55:55Z |
| format | Article |
| id | doaj.art-2f9686dec4134b80a1b30c01f1f30e57 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-10T18:55:55Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2f9686dec4134b80a1b30c01f1f30e572022-12-22T01:37:09ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-08-01710.3389/fvets.2020.00498553840Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3DRodrigo Cañas-Arranz0Patricia de León1Mar Forner2Sira Defaus3María J. Bustos4Elisa Torres5David Andreu6Esther Blanco7Francisco Sobrino8Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Madrid, SpainCentro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Madrid, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, SpainCentro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Madrid, SpainCentro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Madrid, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), Madrid, SpainCentro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Madrid, SpainSynthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140–158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21–35)], has been shown to protect pigs against viral challenge. Interestingly, the modular design of this dendrimer peptide allows modifications aimed at improving its immunogenicity, such as the replacement of the T-cell epitope moiety. Here, we report that a dendrimer peptide, B2T-3D, harboring a T-cell epitope from FMDV 3D protein [3D (56–70)], when inoculated in pigs, elicited consistent levels of neutralizing antibodies and high frequencies of IFN-γ-producing cells upon in vitro recall with the homologous dendrimers, both responses being similar to those evoked by B2T-3A. Lymphocytes from B2T-3A-immunized pigs were in vitro-stimulated by T-3A peptide and to a lesser extent by B-peptide, while those from B2T-3D- immunized animals preferentially recognized the T-3D peptide, suggesting that this epitope is a potent inducer of IFN-γ producing-cells. These results extend the repertoire of T-cell epitopes efficiently recognized by swine lymphocytes and open the possibility of using T-3D to enhance the immunogenicity and the protection conferred by B2T-dendrimers.https://www.frontiersin.org/article/10.3389/fvets.2020.00498/fullFMDVvaccinesdendrimer peptidesT-cell epitopesswine |
| spellingShingle | Rodrigo Cañas-Arranz Patricia de León Mar Forner Sira Defaus María J. Bustos Elisa Torres David Andreu Esther Blanco Francisco Sobrino Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D Frontiers in Veterinary Science FMDV vaccines dendrimer peptides T-cell epitopes swine |
| title | Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D |
| title_full | Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D |
| title_fullStr | Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D |
| title_full_unstemmed | Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D |
| title_short | Immunogenicity of a Dendrimer B2T Peptide Harboring a T-Cell Epitope From FMDV Non-structural Protein 3D |
| title_sort | immunogenicity of a dendrimer b2t peptide harboring a t cell epitope from fmdv non structural protein 3d |
| topic | FMDV vaccines dendrimer peptides T-cell epitopes swine |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00498/full |
| work_keys_str_mv | AT rodrigocanasarranz immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT patriciadeleon immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT marforner immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT siradefaus immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT mariajbustos immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT elisatorres immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT davidandreu immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT estherblanco immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d AT franciscosobrino immunogenicityofadendrimerb2tpeptideharboringatcellepitopefromfmdvnonstructuralprotein3d |