Regulation of transgene expression in genetic immunization
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic im...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1999-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003 |
| _version_ | 1819038506628939776 |
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| author | Harms J.S. Oliveira S.C. Splitter G.A. |
| author_facet | Harms J.S. Oliveira S.C. Splitter G.A. |
| author_sort | Harms J.S. |
| collection | DOAJ |
| description | The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-<FONT FACE="Symbol">g</FONT> on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major histocompatibility complex class I (MHC I) gene. We transfected the luciferase gene driven by these three promoters into 14 cell lines of many tissues and several species. Luciferase assays of transfected cells untreated or treated with IFN-<FONT FACE="Symbol">g</FONT> indicated that although the viral promoters could drive luciferase production in all cell lines tested to higher or lower levels than the MHC I promoter, treatment with IFN-<FONT FACE="Symbol">g</FONT> inhibited transgene expression in most of the cell lines and amplification of the MHC I promoter-driven transgene expression in all cell lines. These data indicate that the SV40 and CMV promoter/enhancers may not be a suitable choice for gene delivery especially for genetic immunization or cancer cytokine gene therapy. The MHC I promoter/enhancer, on the other hand, may be an ideal transgene promoter for applications involving the immune system. |
| first_indexed | 2024-12-21T08:38:24Z |
| format | Article |
| id | doaj.art-2f996d2f9fa9410a8edd6572f1cccc15 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-21T08:38:24Z |
| publishDate | 1999-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-2f996d2f9fa9410a8edd6572f1cccc152022-12-21T19:10:01ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73101999-01-01322Regulation of transgene expression in genetic immunizationHarms J.S.Oliveira S.C.Splitter G.A.The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-<FONT FACE="Symbol">g</FONT> on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major histocompatibility complex class I (MHC I) gene. We transfected the luciferase gene driven by these three promoters into 14 cell lines of many tissues and several species. Luciferase assays of transfected cells untreated or treated with IFN-<FONT FACE="Symbol">g</FONT> indicated that although the viral promoters could drive luciferase production in all cell lines tested to higher or lower levels than the MHC I promoter, treatment with IFN-<FONT FACE="Symbol">g</FONT> inhibited transgene expression in most of the cell lines and amplification of the MHC I promoter-driven transgene expression in all cell lines. These data indicate that the SV40 and CMV promoter/enhancers may not be a suitable choice for gene delivery especially for genetic immunization or cancer cytokine gene therapy. The MHC I promoter/enhancer, on the other hand, may be an ideal transgene promoter for applications involving the immune system.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003DNA vaccinegene therapyregulationtranscriptiontransgene |
| spellingShingle | Harms J.S. Oliveira S.C. Splitter G.A. Regulation of transgene expression in genetic immunization Brazilian Journal of Medical and Biological Research DNA vaccine gene therapy regulation transcription transgene |
| title | Regulation of transgene expression in genetic immunization |
| title_full | Regulation of transgene expression in genetic immunization |
| title_fullStr | Regulation of transgene expression in genetic immunization |
| title_full_unstemmed | Regulation of transgene expression in genetic immunization |
| title_short | Regulation of transgene expression in genetic immunization |
| title_sort | regulation of transgene expression in genetic immunization |
| topic | DNA vaccine gene therapy regulation transcription transgene |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003 |
| work_keys_str_mv | AT harmsjs regulationoftransgeneexpressioningeneticimmunization AT oliveirasc regulationoftransgeneexpressioningeneticimmunization AT splitterga regulationoftransgeneexpressioningeneticimmunization |