Effects of terahertz exposure on skin injury in mouse model
Objective To explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury, and investigate the underlying mechanisms. Methods The hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation, at an average power density of 0, 25 and 50 mW/cm2, respectiv...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Third Military Medical University
2020-12-01
|
| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202008138.htm |
| _version_ | 1818875318348283904 |
|---|---|
| author | CHEN Chunhai MA Qinlong TAO Jiawen LU Yonghui LIN Min GAO Peng |
| author_facet | CHEN Chunhai MA Qinlong TAO Jiawen LU Yonghui LIN Min GAO Peng |
| author_sort | CHEN Chunhai |
| collection | DOAJ |
| description | Objective To explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury, and investigate the underlying mechanisms. Methods The hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation, at an average power density of 0, 25 and 50 mW/cm2, respectively, for 5 min. The temperature curve and pathological changes in the exposed area were observed and analyzed. The gene expression in the skin area was detected using RNA-seq analysis and bioinformatics analysis for global changes of the impairment and underlying molecular mechanisms. Finally, ELISA was used to verify inflammatory responses in the skin revealed by RNA-seq. Results Though 25 mW/cm2 THz irradiation did not induce obvious temperature change, 50 mW/cm2 THz irradiation exposure significantly raised the temperature higher than 2 ℃, and caused epithelial detachment and bleeding in the exposed area. RNA-seq analysis found 49 genes up-regulated and 27 genes down-regulated in the injured skin area. The following Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these genes were majorly involved in immune responses and inflammatory responses. The results of ELISA indicated that the expression levels of IL-1β, IL-6, and TNF-α were increased remarkably after exposure. Conclusion THz irradiation exposure causes significant thermal effects and other injuries, including inflammatory responses, and induces abnormal expression of a series of genes in the exposed skin area. |
| first_indexed | 2024-12-19T13:24:35Z |
| format | Article |
| id | doaj.art-2f9bea924675489287845a2882594783 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-19T13:24:35Z |
| publishDate | 2020-12-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-2f9bea924675489287845a28825947832022-12-21T20:19:36ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-12-0142232282228910.16016/j.1000-5404.202008138Effects of terahertz exposure on skin injury in mouse modelCHEN Chunhai0 MA Qinlong1TAO Jiawen2LU Yonghui3LIN Min4GAO Peng5Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaObjective To explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury, and investigate the underlying mechanisms. Methods The hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation, at an average power density of 0, 25 and 50 mW/cm2, respectively, for 5 min. The temperature curve and pathological changes in the exposed area were observed and analyzed. The gene expression in the skin area was detected using RNA-seq analysis and bioinformatics analysis for global changes of the impairment and underlying molecular mechanisms. Finally, ELISA was used to verify inflammatory responses in the skin revealed by RNA-seq. Results Though 25 mW/cm2 THz irradiation did not induce obvious temperature change, 50 mW/cm2 THz irradiation exposure significantly raised the temperature higher than 2 ℃, and caused epithelial detachment and bleeding in the exposed area. RNA-seq analysis found 49 genes up-regulated and 27 genes down-regulated in the injured skin area. The following Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these genes were majorly involved in immune responses and inflammatory responses. The results of ELISA indicated that the expression levels of IL-1β, IL-6, and TNF-α were increased remarkably after exposure. Conclusion THz irradiation exposure causes significant thermal effects and other injuries, including inflammatory responses, and induces abnormal expression of a series of genes in the exposed skin area.http://aammt.tmmu.edu.cn/Upload/rhtml/202008138.htmterahertzskinthermal effectwounds and injuriesrna-seq analysisinflammatory response |
| spellingShingle | CHEN Chunhai MA Qinlong TAO Jiawen LU Yonghui LIN Min GAO Peng Effects of terahertz exposure on skin injury in mouse model Di-san junyi daxue xuebao terahertz skin thermal effect wounds and injuries rna-seq analysis inflammatory response |
| title | Effects of terahertz exposure on skin injury in mouse model |
| title_full | Effects of terahertz exposure on skin injury in mouse model |
| title_fullStr | Effects of terahertz exposure on skin injury in mouse model |
| title_full_unstemmed | Effects of terahertz exposure on skin injury in mouse model |
| title_short | Effects of terahertz exposure on skin injury in mouse model |
| title_sort | effects of terahertz exposure on skin injury in mouse model |
| topic | terahertz skin thermal effect wounds and injuries rna-seq analysis inflammatory response |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202008138.htm |
| work_keys_str_mv | AT chenchunhai effectsofterahertzexposureonskininjuryinmousemodel AT maqinlong effectsofterahertzexposureonskininjuryinmousemodel AT taojiawen effectsofterahertzexposureonskininjuryinmousemodel AT luyonghui effectsofterahertzexposureonskininjuryinmousemodel AT linmin effectsofterahertzexposureonskininjuryinmousemodel AT gaopeng effectsofterahertzexposureonskininjuryinmousemodel |