The Elephant in the Lab (and Field): Contamination in Aquatic Environmental DNA Studies

The Elephant in the Lab (and Field): Contamination in Aquatic Environmental DNA Studies

The rapid evolution of environmental (e)DNA methods has resulted in knowledge gaps in smaller, yet critical details like proper use of negative controls to detect contamination. Detecting contamination is vital for confident use of eDNA results in decision-making. We conducted two literature reviews...

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Bibliographic Details
Main Authors: Adam J. Sepulveda, Patrick R. Hutchins, Meghan Forstchen, Madeline N. Mckeefry, Anna M. Swigris
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Ecology and Evolution
Subjects:
aquatic
false positive
metabarcoding
negative control (NC)
PCR
targeted
Online Access:https://www.frontiersin.org/articles/10.3389/fevo.2020.609973/full

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