Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i>
Alternative splicing refers to the process of producing different splicing isoforms from the same pre-mRNA through different alternative splicing events, which almost participates in all stages of plant growth and development. In order to understand its role in the fruit development of <i>Osma...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8666 |
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| author | Cancan Ma Cheng Zhang Xiaoyan Wang Fuyuan Zhu Xianrong Wang Min Zhang Yifan Duan |
| author_facet | Cancan Ma Cheng Zhang Xiaoyan Wang Fuyuan Zhu Xianrong Wang Min Zhang Yifan Duan |
| author_sort | Cancan Ma |
| collection | DOAJ |
| description | Alternative splicing refers to the process of producing different splicing isoforms from the same pre-mRNA through different alternative splicing events, which almost participates in all stages of plant growth and development. In order to understand its role in the fruit development of <i>Osmanthus fragrans</i>, transcriptome sequencing and alternative splicing analysis was carried out on three stages of <i>O. fragrans</i> fruit (<i>O. fragrans</i> “Zi Yingui”). The results showed that the proportion of skipping exon events was the highest in all three periods, followed by a retained intron, and the proportion of mutually exclusive exon events was the lowest and most of the alternative splicing events occurred in the first two periods. The results of enrichment analysis of differentially expressed genes and differentially expressed isoforms showed that alpha-Linolenic acid metabolism, flavonoid biosynthesis, carotenoid biosynthesis, photosynthesis, and photosynthetic-antenna protein pathways were significantly enriched, which may play an important role in the fruit development of <i>O. fragrans</i>. The results of this study lay the foundation for further study of the development and maturation of <i>O. fragrans</i> fruit and further ideas for controlling fruit color and improving fruit quality and appearance. |
| first_indexed | 2024-03-11T03:40:21Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:40:21Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2f9eef671925432d8e80974c32dc4a2f2023-11-18T01:39:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410866610.3390/ijms24108666Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i>Cancan Ma0Cheng Zhang1Xiaoyan Wang2Fuyuan Zhu3Xianrong Wang4Min Zhang5Yifan Duan6Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, ChinaAlternative splicing refers to the process of producing different splicing isoforms from the same pre-mRNA through different alternative splicing events, which almost participates in all stages of plant growth and development. In order to understand its role in the fruit development of <i>Osmanthus fragrans</i>, transcriptome sequencing and alternative splicing analysis was carried out on three stages of <i>O. fragrans</i> fruit (<i>O. fragrans</i> “Zi Yingui”). The results showed that the proportion of skipping exon events was the highest in all three periods, followed by a retained intron, and the proportion of mutually exclusive exon events was the lowest and most of the alternative splicing events occurred in the first two periods. The results of enrichment analysis of differentially expressed genes and differentially expressed isoforms showed that alpha-Linolenic acid metabolism, flavonoid biosynthesis, carotenoid biosynthesis, photosynthesis, and photosynthetic-antenna protein pathways were significantly enriched, which may play an important role in the fruit development of <i>O. fragrans</i>. The results of this study lay the foundation for further study of the development and maturation of <i>O. fragrans</i> fruit and further ideas for controlling fruit color and improving fruit quality and appearance.https://www.mdpi.com/1422-0067/24/10/8666<i>Osmanthus fragrans</i>alternative splicingfruit developmentalpha-linolenic acid metabolismcarotenoid biosynthesis |
| spellingShingle | Cancan Ma Cheng Zhang Xiaoyan Wang Fuyuan Zhu Xianrong Wang Min Zhang Yifan Duan Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> International Journal of Molecular Sciences <i>Osmanthus fragrans</i> alternative splicing fruit development alpha-linolenic acid metabolism carotenoid biosynthesis |
| title | Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> |
| title_full | Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> |
| title_fullStr | Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> |
| title_full_unstemmed | Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> |
| title_short | Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of <i>Osmanthus fragrans</i> |
| title_sort | alternative splicing analysis revealed the role of alpha linolenic acid and carotenoids in fruit development of i osmanthus fragrans i |
| topic | <i>Osmanthus fragrans</i> alternative splicing fruit development alpha-linolenic acid metabolism carotenoid biosynthesis |
| url | https://www.mdpi.com/1422-0067/24/10/8666 |
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