Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection
Maydis leaf blight is a significant disease of maize caused by Bipolaris maydis race T, O and C. Molecular mechanisms regulating defense responses in non-CMS maize towards race O fungus are not fully known. In the present investigation, comparative transcriptome profiling was conducted on a highly r...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024025696 |
| _version_ | 1797259846407421952 |
|---|---|
| author | Shweta Meshram Robin Gogoi Bishnu Maya Bashyal Pranab Kumar Mandal Firoz Hossain Aundy Kumar |
| author_facet | Shweta Meshram Robin Gogoi Bishnu Maya Bashyal Pranab Kumar Mandal Firoz Hossain Aundy Kumar |
| author_sort | Shweta Meshram |
| collection | DOAJ |
| description | Maydis leaf blight is a significant disease of maize caused by Bipolaris maydis race T, O and C. Molecular mechanisms regulating defense responses in non-CMS maize towards race O fungus are not fully known. In the present investigation, comparative transcriptome profiling was conducted on a highly resistant maize genotype SC-7-2-1-2-6-1 against a standard susceptible variety CM 119 at 48 h post inoculation (h PI) along with non-infected control. mRNA sequencing generated 38.4 Gb data, where 9349602 reads were mapped uniquely in SC-7, whereas 2714725 reads were mapped uniquely in CM-119. In inoculated SC-7, the total number of differentially expressed genes (DEGs) against control was 1413, where 1011 were up-regulated, and 402 were down-regulated. In susceptible inoculated genotype CM 119, the number of DEGs against control was 2902, where 1703 were up-, and 1199 were down-regulated. DEGs between inoculated resistant and susceptible genotypes were 10745, where 5343 were up-, and 5402 were down-regulated. The RNA-seq data were validated using RT-qPCR. The key findings are that SC-7 poses a robust plant signaling system mainly induced by oxidation–reduction process and calcium-mediated signaling. It regulates its fitness-related genes efficiently, viz., aldolase 2 gene, isopropanoid, phyto hormones, P450 cytochrome, amino acid synthesis, nitrogen assimilation genes etc. These findings showed more transcriptional changes in the SC-7 genotype, which contains many defence-related genes. They can be explored in future crop development programmes to combat multiple maize diseases. The current finding provides information to elucidate molecular and cellular processes occurring in maize during B. maydis race O infection. |
| first_indexed | 2024-03-07T20:03:53Z |
| format | Article |
| id | doaj.art-2fa27801b1d44f909584d3009f79973d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:15:55Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2fa27801b1d44f909584d3009f79973d2024-03-17T07:55:41ZengElsevierHeliyon2405-84402024-03-01105e26538Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infectionShweta Meshram0Robin Gogoi1Bishnu Maya Bashyal2Pranab Kumar Mandal3Firoz Hossain4Aundy Kumar5Division of Plant Pathology, New Delhi 110 012, IndiaDivision of Plant Pathology, New Delhi 110 012, India; Corresponding author.Division of Plant Pathology, New Delhi 110 012, IndiaICAR-Indian Agricultural Research Institute, New Delhi 110012, India; ICAR- National Institute for Plant Biotechnology, New Delhi 110 012, IndiaDivision of Genetics, New Delhi 110 012, IndiaDivision of Plant Pathology, New Delhi 110 012, IndiaMaydis leaf blight is a significant disease of maize caused by Bipolaris maydis race T, O and C. Molecular mechanisms regulating defense responses in non-CMS maize towards race O fungus are not fully known. In the present investigation, comparative transcriptome profiling was conducted on a highly resistant maize genotype SC-7-2-1-2-6-1 against a standard susceptible variety CM 119 at 48 h post inoculation (h PI) along with non-infected control. mRNA sequencing generated 38.4 Gb data, where 9349602 reads were mapped uniquely in SC-7, whereas 2714725 reads were mapped uniquely in CM-119. In inoculated SC-7, the total number of differentially expressed genes (DEGs) against control was 1413, where 1011 were up-regulated, and 402 were down-regulated. In susceptible inoculated genotype CM 119, the number of DEGs against control was 2902, where 1703 were up-, and 1199 were down-regulated. DEGs between inoculated resistant and susceptible genotypes were 10745, where 5343 were up-, and 5402 were down-regulated. The RNA-seq data were validated using RT-qPCR. The key findings are that SC-7 poses a robust plant signaling system mainly induced by oxidation–reduction process and calcium-mediated signaling. It regulates its fitness-related genes efficiently, viz., aldolase 2 gene, isopropanoid, phyto hormones, P450 cytochrome, amino acid synthesis, nitrogen assimilation genes etc. These findings showed more transcriptional changes in the SC-7 genotype, which contains many defence-related genes. They can be explored in future crop development programmes to combat multiple maize diseases. The current finding provides information to elucidate molecular and cellular processes occurring in maize during B. maydis race O infection.http://www.sciencedirect.com/science/article/pii/S2405844024025696Maydis leaf blightBipolaris maydis race OPlant-fungus interactionMaizeRNA-SeqTranscriptome |
| spellingShingle | Shweta Meshram Robin Gogoi Bishnu Maya Bashyal Pranab Kumar Mandal Firoz Hossain Aundy Kumar Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection Heliyon Maydis leaf blight Bipolaris maydis race O Plant-fungus interaction Maize RNA-Seq Transcriptome |
| title | Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection |
| title_full | Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection |
| title_fullStr | Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection |
| title_full_unstemmed | Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection |
| title_short | Investigation on comparative transcriptome profiling of resistant and susceptible non-CMS maize genotypes during Bipolaris maydis race O infection |
| title_sort | investigation on comparative transcriptome profiling of resistant and susceptible non cms maize genotypes during bipolaris maydis race o infection |
| topic | Maydis leaf blight Bipolaris maydis race O Plant-fungus interaction Maize RNA-Seq Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024025696 |
| work_keys_str_mv | AT shwetameshram investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection AT robingogoi investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection AT bishnumayabashyal investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection AT pranabkumarmandal investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection AT firozhossain investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection AT aundykumar investigationoncomparativetranscriptomeprofilingofresistantandsusceptiblenoncmsmaizegenotypesduringbipolarismaydisraceoinfection |