A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits
Thalassemia is one of the major inherited haematological disorders in the Southeast Asia region. This study explored the potential utility of red blood cell (RBC) parameters and reticulocyte cell population data (CPD) parameters in the differential diagnosis of α and β-thalassaemia traits as a rapid...
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MDPI AG
2021-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/11/2163 |
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| author | Angeli Ambayya Santina Sahibon Thoo Wei Yang Qian-Yun Zhang Rosline Hassan Jameela Sathar |
| author_facet | Angeli Ambayya Santina Sahibon Thoo Wei Yang Qian-Yun Zhang Rosline Hassan Jameela Sathar |
| author_sort | Angeli Ambayya |
| collection | DOAJ |
| description | Thalassemia is one of the major inherited haematological disorders in the Southeast Asia region. This study explored the potential utility of red blood cell (RBC) parameters and reticulocyte cell population data (CPD) parameters in the differential diagnosis of α and β-thalassaemia traits as a rapid and cost-effective tool for screening of thalassemia traits. In this study, a total of 1597 subjects (1394 apparently healthy subjects, 155 subjects with α-thalassaemia trait, and 48 subjects with β-thalassaemia trait) were accrued. The parameters studied were the RBC parameters and reticulocyte CPD parameters derived from Unicel DxH800. A novel algorithm named αβ-algorithm was developed: (MN-LMALS-RET × RDW) − MCH) to discriminate α from β-thalassaemia trait with a cut-off value of 1742.5 [AUC = 0.966, sensitivity = 92%, specificity = 90%, 95% CI = 0.94–0.99]. Two prospective studies were carried: an in-house cohort to assess the specificity of this algorithm in 310 samples comprising various RBC disorders and in an interlaboratory cohort of 65 α-thalassemia trait, and 30 β-thalassaemia trait subjects to assess the reproducibility of the findings. We propose the αβ-algorithm to serve as a rapid, inexpensive surrogate evaluation tool of α and β-thalassaemia in the population screening of thalassemia traits in geographic regions with a high burden of these inherited blood disorders. |
| first_indexed | 2024-03-10T05:34:13Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T05:34:13Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-2fa4a4ae847243d49a362df371ed33442023-11-22T23:03:17ZengMDPI AGDiagnostics2075-44182021-11-011111216310.3390/diagnostics11112163A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia TraitsAngeli Ambayya0Santina Sahibon1Thoo Wei Yang2Qian-Yun Zhang3Rosline Hassan4Jameela Sathar5Haematology Department, Hospital Ampang, Ampang 68000, Selangor, MalaysiaGribbles Pathology Malaysia, Petaling Jaya 46100, Selangor, MalaysiaStraits Scientific Malaysia, Ampang 68000, Selangor, MalaysiaDepartment of Pathology, University of New Mexico, Albuquerque, NM 87131, USADepartment Haematology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 15200, Kelantan, MalaysiaHaematology Department, Hospital Ampang, Ampang 68000, Selangor, MalaysiaThalassemia is one of the major inherited haematological disorders in the Southeast Asia region. This study explored the potential utility of red blood cell (RBC) parameters and reticulocyte cell population data (CPD) parameters in the differential diagnosis of α and β-thalassaemia traits as a rapid and cost-effective tool for screening of thalassemia traits. In this study, a total of 1597 subjects (1394 apparently healthy subjects, 155 subjects with α-thalassaemia trait, and 48 subjects with β-thalassaemia trait) were accrued. The parameters studied were the RBC parameters and reticulocyte CPD parameters derived from Unicel DxH800. A novel algorithm named αβ-algorithm was developed: (MN-LMALS-RET × RDW) − MCH) to discriminate α from β-thalassaemia trait with a cut-off value of 1742.5 [AUC = 0.966, sensitivity = 92%, specificity = 90%, 95% CI = 0.94–0.99]. Two prospective studies were carried: an in-house cohort to assess the specificity of this algorithm in 310 samples comprising various RBC disorders and in an interlaboratory cohort of 65 α-thalassemia trait, and 30 β-thalassaemia trait subjects to assess the reproducibility of the findings. We propose the αβ-algorithm to serve as a rapid, inexpensive surrogate evaluation tool of α and β-thalassaemia in the population screening of thalassemia traits in geographic regions with a high burden of these inherited blood disorders.https://www.mdpi.com/2075-4418/11/11/2163cell population dataalgorithmα-thalassemiaβ-thalassaemiaVCS parameters |
| spellingShingle | Angeli Ambayya Santina Sahibon Thoo Wei Yang Qian-Yun Zhang Rosline Hassan Jameela Sathar A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits Diagnostics cell population data algorithm α-thalassemia β-thalassaemia VCS parameters |
| title | A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits |
| title_full | A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits |
| title_fullStr | A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits |
| title_full_unstemmed | A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits |
| title_short | A Novel Algorithm Using Cell Population Data (VCS Parameters) as a Screening Discriminant between Alpha and Beta Thalassemia Traits |
| title_sort | novel algorithm using cell population data vcs parameters as a screening discriminant between alpha and beta thalassemia traits |
| topic | cell population data algorithm α-thalassemia β-thalassaemia VCS parameters |
| url | https://www.mdpi.com/2075-4418/11/11/2163 |
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