Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation
The grain aphid <i>Sitobion avenae</i> (Fabricius) is one of the most destructive pests of wheat (<i>Triticum aestivum</i>). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to...
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2022-05-01
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| author | Xinlun Liu Xudan Kou Shichao Bai Yufeng Luo Zhenyu Wang Lincai Xie Pingchuan Deng Hong Zhang Changyou Wang Yajuan Wang Jixin Zhao Wanquan Ji |
| author_facet | Xinlun Liu Xudan Kou Shichao Bai Yufeng Luo Zhenyu Wang Lincai Xie Pingchuan Deng Hong Zhang Changyou Wang Yajuan Wang Jixin Zhao Wanquan Ji |
| author_sort | Xinlun Liu |
| collection | DOAJ |
| description | The grain aphid <i>Sitobion avenae</i> (Fabricius) is one of the most destructive pests of wheat (<i>Triticum aestivum</i>). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to this pest. The present study was carried out to investigate the potential of the tetraploid wheat (<i>Triticum turgidum</i>) variety Lanmai, which showed high resistance to <i>S. avenae</i> at both seedling and adult plant stages, as a source of resistance genes. Based on apterous adult aphids’ fecundity tests and choice bioassays, Lanmai has been shown to display antixenosis and antibiosis. Suppression subtractive hybridization (SSH) was employed to identify and isolate the putative candidate defense genes in Lanmai against <i>S. avenae</i> infestation. A total of 134 expressed sequence tags (ESTs) were identified and categorized based on their putative functions. RT-qPCR analysis of 30 selected genes confirmed their differential expression over time between the resistant wheat variety Lanmai and susceptible wheat variety Polan305 during <i>S. avenae</i> infestation. There were 11 genes related to the photosynthesis process, and only 3 genes showed higher expression in Lanmai than in Polan305 after <i>S. avenae</i> infestation. Gene expression analysis also revealed that Lanmai played a critical role in salicylic acid and jasmonic acid pathways after <i>S. avenae</i> infestation. This study provided further insights into the role of defense signaling networks in wheat resistance to <i>S. avenae</i> and indicates that the resistant tetraploid wheat variety Lanmai may provide a valuable resource for aphid tolerance improvement in wheat. |
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| language | English |
| last_indexed | 2024-03-10T01:15:55Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-2fa5cc549c5f4ced95c8df152ede9c562023-11-23T14:08:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311601210.3390/ijms23116012Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) InfestationXinlun Liu0Xudan Kou1Shichao Bai2Yufeng Luo3Zhenyu Wang4Lincai Xie5Pingchuan Deng6Hong Zhang7Changyou Wang8Yajuan Wang9Jixin Zhao10Wanquan Ji11State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaThe grain aphid <i>Sitobion avenae</i> (Fabricius) is one of the most destructive pests of wheat (<i>Triticum aestivum</i>). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to this pest. The present study was carried out to investigate the potential of the tetraploid wheat (<i>Triticum turgidum</i>) variety Lanmai, which showed high resistance to <i>S. avenae</i> at both seedling and adult plant stages, as a source of resistance genes. Based on apterous adult aphids’ fecundity tests and choice bioassays, Lanmai has been shown to display antixenosis and antibiosis. Suppression subtractive hybridization (SSH) was employed to identify and isolate the putative candidate defense genes in Lanmai against <i>S. avenae</i> infestation. A total of 134 expressed sequence tags (ESTs) were identified and categorized based on their putative functions. RT-qPCR analysis of 30 selected genes confirmed their differential expression over time between the resistant wheat variety Lanmai and susceptible wheat variety Polan305 during <i>S. avenae</i> infestation. There were 11 genes related to the photosynthesis process, and only 3 genes showed higher expression in Lanmai than in Polan305 after <i>S. avenae</i> infestation. Gene expression analysis also revealed that Lanmai played a critical role in salicylic acid and jasmonic acid pathways after <i>S. avenae</i> infestation. This study provided further insights into the role of defense signaling networks in wheat resistance to <i>S. avenae</i> and indicates that the resistant tetraploid wheat variety Lanmai may provide a valuable resource for aphid tolerance improvement in wheat.https://www.mdpi.com/1422-0067/23/11/6012aphid resistancetetraploid wheat<i>Sitobion avenae</i>suppression subtractive hybridizationgene expression |
| spellingShingle | Xinlun Liu Xudan Kou Shichao Bai Yufeng Luo Zhenyu Wang Lincai Xie Pingchuan Deng Hong Zhang Changyou Wang Yajuan Wang Jixin Zhao Wanquan Ji Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation International Journal of Molecular Sciences aphid resistance tetraploid wheat <i>Sitobion avenae</i> suppression subtractive hybridization gene expression |
| title | Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation |
| title_full | Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation |
| title_fullStr | Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation |
| title_full_unstemmed | Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation |
| title_short | Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat (<i>Triticum turgidum</i>) under <i>Sitobion avenae</i> (F.) Infestation |
| title_sort | identification of differentially expressed genes in resistant tetraploid wheat i triticum turgidum i under i sitobion avenae i f infestation |
| topic | aphid resistance tetraploid wheat <i>Sitobion avenae</i> suppression subtractive hybridization gene expression |
| url | https://www.mdpi.com/1422-0067/23/11/6012 |
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