miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2
Gastric cancer is one of the most common malignancies harmful to human health. The search for effective drugs or gene therapy has aroused the attention of scientists. So far, microRNAs, as small non-coding RNAs, have the potential to be therapeutic targets for cancer. Herein, we found a highly expre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.690196/full |
| _version_ | 1818716432323575808 |
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| author | Liuqing Yang Lina Li Pan Chang Ming Wei Jianting Chen Chaofan Zhu Jing Jia |
| author_facet | Liuqing Yang Lina Li Pan Chang Ming Wei Jianting Chen Chaofan Zhu Jing Jia |
| author_sort | Liuqing Yang |
| collection | DOAJ |
| description | Gastric cancer is one of the most common malignancies harmful to human health. The search for effective drugs or gene therapy has aroused the attention of scientists. So far, microRNAs, as small non-coding RNAs, have the potential to be therapeutic targets for cancer. Herein, we found a highly expressed miR-25 in gastric cancer cell. However, the function of miR-25 for gastric cancer cell growth and apoptosis was unknown. Functionally, we used RT-qPCR, western blot, CCK-8, and flow cytometry to detect gastric cancer cell growth and apoptosis. The results indicated that miR-25 promoted gastric cancer cell growth and inhibited their apoptosis. Mechanistically, we found that a gene EGR2 was a potential target gene of miR-25. Further dual-luciferase results supported this prediction. Moreover, knockdown of EGR2 promoted gastric cancer cell growth and inhibited their apoptosis by flow cytometry detection. Altogether, these findings revealed miR-25 as a regulator of gastric cancer cell growth and apoptosis through targeting EGR2. |
| first_indexed | 2024-12-17T19:19:10Z |
| format | Article |
| id | doaj.art-2fad3862537e43f8b8171f43068d51fb |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T19:19:10Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-2fad3862537e43f8b8171f43068d51fb2022-12-21T21:35:39ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-10-011210.3389/fgene.2021.690196690196miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2Liuqing Yang0Lina Li1Pan Chang2Ming Wei3Jianting Chen4Chaofan Zhu5Jing Jia6Second Affiliated Hospital of Xi’an Medical University, Xi’ an, ChinaFirst Department of Medical Oncology, Affiliated Shaanxi Provincial Cancer Hospital, Xi’an, ChinaSecond Affiliated Hospital of Xi’an Medical University, Xi’ an, ChinaDepartment of Pharmacology, Xi’an Medical University, Xi’an, ChinaSecond Affiliated Hospital of Xi’an Medical University, Xi’ an, ChinaSecond Affiliated Hospital of Xi’an Medical University, Xi’ an, ChinaSecond Affiliated Hospital of Xi’an Medical University, Xi’ an, ChinaGastric cancer is one of the most common malignancies harmful to human health. The search for effective drugs or gene therapy has aroused the attention of scientists. So far, microRNAs, as small non-coding RNAs, have the potential to be therapeutic targets for cancer. Herein, we found a highly expressed miR-25 in gastric cancer cell. However, the function of miR-25 for gastric cancer cell growth and apoptosis was unknown. Functionally, we used RT-qPCR, western blot, CCK-8, and flow cytometry to detect gastric cancer cell growth and apoptosis. The results indicated that miR-25 promoted gastric cancer cell growth and inhibited their apoptosis. Mechanistically, we found that a gene EGR2 was a potential target gene of miR-25. Further dual-luciferase results supported this prediction. Moreover, knockdown of EGR2 promoted gastric cancer cell growth and inhibited their apoptosis by flow cytometry detection. Altogether, these findings revealed miR-25 as a regulator of gastric cancer cell growth and apoptosis through targeting EGR2.https://www.frontiersin.org/articles/10.3389/fgene.2021.690196/fullgastric cancerMiR-25cell growthapoptosisEgr2 |
| spellingShingle | Liuqing Yang Lina Li Pan Chang Ming Wei Jianting Chen Chaofan Zhu Jing Jia miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 Frontiers in Genetics gastric cancer MiR-25 cell growth apoptosis Egr2 |
| title | miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 |
| title_full | miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 |
| title_fullStr | miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 |
| title_full_unstemmed | miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 |
| title_short | miR-25 Regulates Gastric Cancer Cell Growth and Apoptosis by Targeting EGR2 |
| title_sort | mir 25 regulates gastric cancer cell growth and apoptosis by targeting egr2 |
| topic | gastric cancer MiR-25 cell growth apoptosis Egr2 |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.690196/full |
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