Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production
Abstract Background Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) is an entomopathogenic fungus that has potential as a biological control agent against many insect pests. This study was conducted to optimize the conditions for large-scale production on rice of B. bassiana and evaluate i...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-07-01
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| Series: | Egyptian Journal of Biological Pest Control |
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| Online Access: | https://doi.org/10.1186/s41938-022-00584-w |
| _version_ | 1828427015554859008 |
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| author | Ibrahima Camara Kaili Cao Rouguiatou Sangbaramou Peipei Wu Wangpeng Shi Shuqian Tan |
| author_facet | Ibrahima Camara Kaili Cao Rouguiatou Sangbaramou Peipei Wu Wangpeng Shi Shuqian Tan |
| author_sort | Ibrahima Camara |
| collection | DOAJ |
| description | Abstract Background Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) is an entomopathogenic fungus that has potential as a biological control agent against many insect pests. This study was conducted to optimize the conditions for large-scale production on rice of B. bassiana and evaluate its virulence against the bean flower thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae). Results The optimal substrate quantity for conidia production was 200 g of rice per container (2.1 l), with substrate having a 52% moisture content, being supplemented with 2% cooking oil. In stage production of a batch, 150 ml of conidia suspension (at 24.33 × 107 spores/ml) was incubated for 10 days in SDA liquid medium amended with 4% glucose and 1.5% yeast extract. The SDA medium was then used to inoculate one container of the rice substrate. The optimum ratio of conidial suspension to substrate (v/v) was 20–25%. Virulence of the spore powder harvested from the rice substrate was same as that of produced on the SDA solid media against thrips. The level of control from an application of spore powder of the thrips M. usitatus was similar to that from chemical the pesticide spinetoram when applied in a cowpea (Vigna sinensis Endl) field. Conclusions The optimized rice substrate system can be used for a large-scale production of B. bassiana spores, which can be used for field control of thrips. |
| first_indexed | 2024-12-10T16:55:04Z |
| format | Article |
| id | doaj.art-2fb0125ec17f48a3b5ba54253b421905 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-10T16:55:04Z |
| publishDate | 2022-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-2fb0125ec17f48a3b5ba54253b4219052022-12-22T01:40:46ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-07-013211810.1186/s41938-022-00584-wScreening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale productionIbrahima Camara0Kaili Cao1Rouguiatou Sangbaramou2Peipei Wu3Wangpeng Shi4Shuqian Tan5Department of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityDepartment of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityDepartment of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityDepartment of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityDepartment of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityDepartment of Entomology and MOA, Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityAbstract Background Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) is an entomopathogenic fungus that has potential as a biological control agent against many insect pests. This study was conducted to optimize the conditions for large-scale production on rice of B. bassiana and evaluate its virulence against the bean flower thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae). Results The optimal substrate quantity for conidia production was 200 g of rice per container (2.1 l), with substrate having a 52% moisture content, being supplemented with 2% cooking oil. In stage production of a batch, 150 ml of conidia suspension (at 24.33 × 107 spores/ml) was incubated for 10 days in SDA liquid medium amended with 4% glucose and 1.5% yeast extract. The SDA medium was then used to inoculate one container of the rice substrate. The optimum ratio of conidial suspension to substrate (v/v) was 20–25%. Virulence of the spore powder harvested from the rice substrate was same as that of produced on the SDA solid media against thrips. The level of control from an application of spore powder of the thrips M. usitatus was similar to that from chemical the pesticide spinetoram when applied in a cowpea (Vigna sinensis Endl) field. Conclusions The optimized rice substrate system can be used for a large-scale production of B. bassiana spores, which can be used for field control of thrips.https://doi.org/10.1186/s41938-022-00584-wEntomopathogenic fungiMass productionRice substrateVirulenceThrips |
| spellingShingle | Ibrahima Camara Kaili Cao Rouguiatou Sangbaramou Peipei Wu Wangpeng Shi Shuqian Tan Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production Egyptian Journal of Biological Pest Control Entomopathogenic fungi Mass production Rice substrate Virulence Thrips |
| title | Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production |
| title_full | Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production |
| title_fullStr | Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production |
| title_full_unstemmed | Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production |
| title_short | Screening of Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) strains against Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) and conditions for large-scale production |
| title_sort | screening of beauveria bassiana bals hypocreales cordycipitaceae strains against megalurothrips usitatus bagnall thysanoptera thripidae and conditions for large scale production |
| topic | Entomopathogenic fungi Mass production Rice substrate Virulence Thrips |
| url | https://doi.org/10.1186/s41938-022-00584-w |
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