StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid
The major stages of the potato life cycle are tuber dormancy and sprouting, however, there is still known very little of the mechanisms that control these processes. TCP (Theosinte branch I, Cycloidea, proliferationcell factors 1 and 2) transcription factors play a key role in plant growth and dorma...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1009552/full |
| _version_ | 1798033832629239808 |
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| author | Kaitong Wang Kaitong Wang Ning Zhang Ning Zhang Xue Fu Xue Fu Huanhuan Zhang Huanhuan Zhang Shengyan Liu Shengyan Liu Xue Pu Xiao Wang Huaijun Si Huaijun Si |
| author_facet | Kaitong Wang Kaitong Wang Ning Zhang Ning Zhang Xue Fu Xue Fu Huanhuan Zhang Huanhuan Zhang Shengyan Liu Shengyan Liu Xue Pu Xiao Wang Huaijun Si Huaijun Si |
| author_sort | Kaitong Wang |
| collection | DOAJ |
| description | The major stages of the potato life cycle are tuber dormancy and sprouting, however, there is still known very little of the mechanisms that control these processes. TCP (Theosinte branch I, Cycloidea, proliferationcell factors 1 and 2) transcription factors play a key role in plant growth and dormancy related developmental processes. Previous researches demonstrated that TCP transcription factor StTCP15 had a function in the promotion of dormancy. To elucidate the function of StTCP15 gene, it was cloned from potato cultivar “Desiree,” which encodes a polypeptide consisting of 414 amino acids and is mainly found in the nucleus. The potato tubers of StTCP15 overexpression lines sprouted in advance, while the potato tubers of StTCP15 down-regulated expression lines showed delayed sprouting. In addition, it was also found that overexpression lines of StTCP15 extremely significantly reduced the ratio of abscisic acid (ABA)/gibberellic acid (GA3), while the superoxide dismutase activity decreased, and the activity of peroxidase and catalase increased compared with the wild type. The opposite result was found in the down-regulated expression lines of StTCP15 gene. Three interacting proteins, StSnRK1, StF-Box and StGID1, were screened by Yeast two-hybrid, and verified by Bimolecular Fluorescence Complementation and Split-luciferase, indicating that StTCP15 could affect ABA and GA3 signaling pathways to regulate potato tuber dormancy and sprouting. Together, these results demonstrated that StTCP15 regulated potato tuber dormancy and sprouting by affecting the dynamic balance between ABA and GA3. The result could provide some information on the molecular mechanism of StTCP15 regulating potato tuber dormancy and sprouting. |
| first_indexed | 2024-04-11T20:34:58Z |
| format | Article |
| id | doaj.art-2fb0689aa1ad4a58b4a4d00d03a04f26 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T20:34:58Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2fb0689aa1ad4a58b4a4d00d03a04f262022-12-22T04:04:23ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10095521009552StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acidKaitong Wang0Kaitong Wang1Ning Zhang2Ning Zhang3Xue Fu4Xue Fu5Huanhuan Zhang6Huanhuan Zhang7Shengyan Liu8Shengyan Liu9Xue Pu10Xiao Wang11Huaijun Si12Huaijun Si13State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaThe major stages of the potato life cycle are tuber dormancy and sprouting, however, there is still known very little of the mechanisms that control these processes. TCP (Theosinte branch I, Cycloidea, proliferationcell factors 1 and 2) transcription factors play a key role in plant growth and dormancy related developmental processes. Previous researches demonstrated that TCP transcription factor StTCP15 had a function in the promotion of dormancy. To elucidate the function of StTCP15 gene, it was cloned from potato cultivar “Desiree,” which encodes a polypeptide consisting of 414 amino acids and is mainly found in the nucleus. The potato tubers of StTCP15 overexpression lines sprouted in advance, while the potato tubers of StTCP15 down-regulated expression lines showed delayed sprouting. In addition, it was also found that overexpression lines of StTCP15 extremely significantly reduced the ratio of abscisic acid (ABA)/gibberellic acid (GA3), while the superoxide dismutase activity decreased, and the activity of peroxidase and catalase increased compared with the wild type. The opposite result was found in the down-regulated expression lines of StTCP15 gene. Three interacting proteins, StSnRK1, StF-Box and StGID1, were screened by Yeast two-hybrid, and verified by Bimolecular Fluorescence Complementation and Split-luciferase, indicating that StTCP15 could affect ABA and GA3 signaling pathways to regulate potato tuber dormancy and sprouting. Together, these results demonstrated that StTCP15 regulated potato tuber dormancy and sprouting by affecting the dynamic balance between ABA and GA3. The result could provide some information on the molecular mechanism of StTCP15 regulating potato tuber dormancy and sprouting.https://www.frontiersin.org/articles/10.3389/fpls.2022.1009552/fullpotatoStTCP15dormancysproutingABAGA3 |
| spellingShingle | Kaitong Wang Kaitong Wang Ning Zhang Ning Zhang Xue Fu Xue Fu Huanhuan Zhang Huanhuan Zhang Shengyan Liu Shengyan Liu Xue Pu Xiao Wang Huaijun Si Huaijun Si StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid Frontiers in Plant Science potato StTCP15 dormancy sprouting ABA GA3 |
| title | StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| title_full | StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| title_fullStr | StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| title_full_unstemmed | StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| title_short | StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| title_sort | sttcp15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid |
| topic | potato StTCP15 dormancy sprouting ABA GA3 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1009552/full |
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