iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.).
Vernalization is classically defined as the induction of flowering process by exposure of the plants to a prolonged cold condition. Normally, it is considered as a precondition of flowering. Vicia faba, commonly known as faba bean, belongs to family Fabaceae. It is one of the plant species that has...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5679601?pdf=render |
| _version_ | 1819108133983748096 |
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| author | Yun-Ying Cao Xiao-Chun Bian Mo-Xian Chen Li-Ru Xia Jianhua Zhang Fu-Yuan Zhu Chun-Fang Wu |
| author_facet | Yun-Ying Cao Xiao-Chun Bian Mo-Xian Chen Li-Ru Xia Jianhua Zhang Fu-Yuan Zhu Chun-Fang Wu |
| author_sort | Yun-Ying Cao |
| collection | DOAJ |
| description | Vernalization is classically defined as the induction of flowering process by exposure of the plants to a prolonged cold condition. Normally, it is considered as a precondition of flowering. Vicia faba, commonly known as faba bean, belongs to family Fabaceae. It is one of the plant species that has been cultivated in the earliest human settlements. In this study, an iTRAQ-LC-MS/MS-based quantitative proteomic analysis has been conducted to compare the vernalized faba bean seedlings and its corresponding control. In total, 91 proteins from various functional categories were observed to be differentially accumulated in vernalized faba bean seedlings. Subsequent gene ontology analysis indicated that several biological processes or metabolic pathways including photosynthesis and phytic acid metabolism were differentially respond to vernalization in comparison to the control sample. Further investigation revealed that a family of proteins nominated as glycine-rich RNA-binding factor was accumulated in vernalized seedlings, indicating an extra layer of regulation by alternative splicing on transcript abundance in response to vernalization. These findings raise a possibility that these candidate proteins could be important to represent the responsive network under vernalization process. Therefore, we propose that the regulation of vernalization in faba bean not only occurs at the transcriptional level as previously reported, but also at the post-transcriptional level. |
| first_indexed | 2024-12-22T03:05:06Z |
| format | Article |
| id | doaj.art-2fb06afccde14bc48b12c6cc9e32c787 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:05:06Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2fb06afccde14bc48b12c6cc9e32c7872022-12-21T18:41:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018743610.1371/journal.pone.0187436iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.).Yun-Ying CaoXiao-Chun BianMo-Xian ChenLi-Ru XiaJianhua ZhangFu-Yuan ZhuChun-Fang WuVernalization is classically defined as the induction of flowering process by exposure of the plants to a prolonged cold condition. Normally, it is considered as a precondition of flowering. Vicia faba, commonly known as faba bean, belongs to family Fabaceae. It is one of the plant species that has been cultivated in the earliest human settlements. In this study, an iTRAQ-LC-MS/MS-based quantitative proteomic analysis has been conducted to compare the vernalized faba bean seedlings and its corresponding control. In total, 91 proteins from various functional categories were observed to be differentially accumulated in vernalized faba bean seedlings. Subsequent gene ontology analysis indicated that several biological processes or metabolic pathways including photosynthesis and phytic acid metabolism were differentially respond to vernalization in comparison to the control sample. Further investigation revealed that a family of proteins nominated as glycine-rich RNA-binding factor was accumulated in vernalized seedlings, indicating an extra layer of regulation by alternative splicing on transcript abundance in response to vernalization. These findings raise a possibility that these candidate proteins could be important to represent the responsive network under vernalization process. Therefore, we propose that the regulation of vernalization in faba bean not only occurs at the transcriptional level as previously reported, but also at the post-transcriptional level.http://europepmc.org/articles/PMC5679601?pdf=render |
| spellingShingle | Yun-Ying Cao Xiao-Chun Bian Mo-Xian Chen Li-Ru Xia Jianhua Zhang Fu-Yuan Zhu Chun-Fang Wu iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). PLoS ONE |
| title | iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). |
| title_full | iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). |
| title_fullStr | iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). |
| title_full_unstemmed | iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). |
| title_short | iTRAQ-based quantitative proteomic analysis in vernalization-treated faba bean (Vicia faba L.). |
| title_sort | itraq based quantitative proteomic analysis in vernalization treated faba bean vicia faba l |
| url | http://europepmc.org/articles/PMC5679601?pdf=render |
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