Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot
In order to increase yield,take the Bacillus subtilis Asr as the expreession strain, the fermentation process of Bacillus subtilis Asr was optimized by orthogonal test and response surface method. The results show that three main factors affecting bacterial yield are leaching yeast powder, MgSO4 and...
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Hebei University of Science and Technology
2020-10-01
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| Series: | Journal of Hebei University of Science and Technology |
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| Online Access: | http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b202005007&flag=1&journal_ |
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| author | Yuwen REN Yuanyuan REN Yazhen LIU Tianhua LU Liqiang LIU Xiaohui ZHOU |
| author_facet | Yuwen REN Yuanyuan REN Yazhen LIU Tianhua LU Liqiang LIU Xiaohui ZHOU |
| author_sort | Yuwen REN |
| collection | DOAJ |
| description | In order to increase yield,take the Bacillus subtilis Asr as the expreession strain, the fermentation process of Bacillus subtilis Asr was optimized by orthogonal test and response surface method. The results show that three main factors affecting bacterial yield are leaching yeast powder, MgSO4 and CaCl2. The best fermentation medium formulation is as following: sucrose 20 g/L, peptone 10 g/L, leaching yeast powder 8.65 g/L, K2HPO4 3.0 g/L, MgSO4 0.27 g/L and CaCl2 0.53 g/L. The optimal culture conditions are as following: 37 ℃, initial pH 7.0, inoculum of 10% and stirring speed of 250 r/min. After the optimization, the highest yield of Bacillus subtilis Asr reaches 7.30×108</sup> cfu/mL, which is 3.95 times higher than that before optimization. The study provides a technical reference for the subsequent bacterial scale-up culture in fermentor and the optimization of fermentation medium from other Bacillus subtilis. |
| first_indexed | 2024-12-23T10:23:46Z |
| format | Article |
| id | doaj.art-2fb0b1f9e8a047bab128dadb796cb676 |
| institution | Directory Open Access Journal |
| issn | 1008-1542 |
| language | zho |
| last_indexed | 2024-12-23T10:23:46Z |
| publishDate | 2020-10-01 |
| publisher | Hebei University of Science and Technology |
| record_format | Article |
| series | Journal of Hebei University of Science and Technology |
| spelling | doaj.art-2fb0b1f9e8a047bab128dadb796cb6762022-12-21T17:50:37ZzhoHebei University of Science and TechnologyJournal of Hebei University of Science and Technology1008-15422020-10-0141543344110.7535/hbkd.2020yx05007b202005007Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rotYuwen REN0Yuanyuan REN1Yazhen LIU2Tianhua LU3Liqiang LIU4Xiaohui ZHOU5School of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaSchool of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaAgricultural and Rural Bureau of Chongli District in Zhangjiakou City, Zhangjiakou, Hebei 076350, ChinaSchool of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaSchool of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaSchool of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaIn order to increase yield,take the Bacillus subtilis Asr as the expreession strain, the fermentation process of Bacillus subtilis Asr was optimized by orthogonal test and response surface method. The results show that three main factors affecting bacterial yield are leaching yeast powder, MgSO4 and CaCl2. The best fermentation medium formulation is as following: sucrose 20 g/L, peptone 10 g/L, leaching yeast powder 8.65 g/L, K2HPO4 3.0 g/L, MgSO4 0.27 g/L and CaCl2 0.53 g/L. The optimal culture conditions are as following: 37 ℃, initial pH 7.0, inoculum of 10% and stirring speed of 250 r/min. After the optimization, the highest yield of Bacillus subtilis Asr reaches 7.30×108</sup> cfu/mL, which is 3.95 times higher than that before optimization. The study provides a technical reference for the subsequent bacterial scale-up culture in fermentor and the optimization of fermentation medium from other Bacillus subtilis.http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b202005007&flag=1&journal_fermentation engineering; bacillus subtilis; fermentation process; orthogonal method; response surface method; optimization |
| spellingShingle | Yuwen REN Yuanyuan REN Yazhen LIU Tianhua LU Liqiang LIU Xiaohui ZHOU Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot Journal of Hebei University of Science and Technology fermentation engineering; bacillus subtilis; fermentation process; orthogonal method; response surface method; optimization |
| title | Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot |
| title_full | Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot |
| title_fullStr | Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot |
| title_full_unstemmed | Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot |
| title_short | Optimization of high-density fermentation for Bacillus subtilis resistant to plant soft rot |
| title_sort | optimization of high density fermentation for bacillus subtilis resistant to plant soft rot |
| topic | fermentation engineering; bacillus subtilis; fermentation process; orthogonal method; response surface method; optimization |
| url | http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b202005007&flag=1&journal_ |
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