The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission
The Endosomal Sorting Complexes Required for Transport (ESCRT) machinery mediates the membrane fission step that completes cytokinetic abscission and separates dividing cells. Filaments composed of ESCRT-III subunits constrict membranes of the intercellular bridge midbody to the abscission point. Th...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/84515 |
| _version_ | 1827789478164430848 |
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| author | Elliott L Paine Jack J Skalicky Frank G Whitby Douglas R Mackay Katharine S Ullman Christopher P Hill Wesley I Sundquist |
| author_facet | Elliott L Paine Jack J Skalicky Frank G Whitby Douglas R Mackay Katharine S Ullman Christopher P Hill Wesley I Sundquist |
| author_sort | Elliott L Paine |
| collection | DOAJ |
| description | The Endosomal Sorting Complexes Required for Transport (ESCRT) machinery mediates the membrane fission step that completes cytokinetic abscission and separates dividing cells. Filaments composed of ESCRT-III subunits constrict membranes of the intercellular bridge midbody to the abscission point. These filaments also bind and recruit cofactors whose activities help execute abscission and/or delay abscission timing in response to mitotic errors via the NoCut/Abscission checkpoint. We previously showed that the ESCRT-III subunit IST1 binds the cysteine protease Calpain-7 (CAPN7) and that CAPN7 is required for both efficient abscission and NoCut checkpoint maintenance (Wenzel et al., 2022). Here, we report biochemical and crystallographic studies showing that the tandem microtubule-interacting and trafficking (MIT) domains of CAPN7 bind simultaneously to two distinct IST1 MIT interaction motifs. Structure-guided point mutations in either CAPN7 MIT domain disrupted IST1 binding in vitro and in cells, and depletion/rescue experiments showed that the CAPN7-IST1 interaction is required for (1) CAPN7 recruitment to midbodies, (2) efficient abscission, and (3) NoCut checkpoint arrest. CAPN7 proteolytic activity is also required for abscission and checkpoint maintenance. Hence, IST1 recruits CAPN7 to midbodies, where its proteolytic activity is required to regulate and complete abscission. |
| first_indexed | 2024-03-11T17:20:21Z |
| format | Article |
| id | doaj.art-2fc3d4be4cff4d3e80b6e77ad5bf983b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-11T17:20:21Z |
| publishDate | 2023-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2fc3d4be4cff4d3e80b6e77ad5bf983b2023-10-19T13:26:08ZengeLife Sciences Publications LtdeLife2050-084X2023-09-011210.7554/eLife.84515The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscissionElliott L Paine0https://orcid.org/0000-0001-5575-297XJack J Skalicky1https://orcid.org/0000-0002-5450-0567Frank G Whitby2https://orcid.org/0000-0003-3511-2216Douglas R Mackay3Katharine S Ullman4https://orcid.org/0000-0003-3693-2830Christopher P Hill5https://orcid.org/0000-0001-6796-7740Wesley I Sundquist6https://orcid.org/0000-0001-9988-6021Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesDepartment of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, United StatesDepartment of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, United StatesThe Endosomal Sorting Complexes Required for Transport (ESCRT) machinery mediates the membrane fission step that completes cytokinetic abscission and separates dividing cells. Filaments composed of ESCRT-III subunits constrict membranes of the intercellular bridge midbody to the abscission point. These filaments also bind and recruit cofactors whose activities help execute abscission and/or delay abscission timing in response to mitotic errors via the NoCut/Abscission checkpoint. We previously showed that the ESCRT-III subunit IST1 binds the cysteine protease Calpain-7 (CAPN7) and that CAPN7 is required for both efficient abscission and NoCut checkpoint maintenance (Wenzel et al., 2022). Here, we report biochemical and crystallographic studies showing that the tandem microtubule-interacting and trafficking (MIT) domains of CAPN7 bind simultaneously to two distinct IST1 MIT interaction motifs. Structure-guided point mutations in either CAPN7 MIT domain disrupted IST1 binding in vitro and in cells, and depletion/rescue experiments showed that the CAPN7-IST1 interaction is required for (1) CAPN7 recruitment to midbodies, (2) efficient abscission, and (3) NoCut checkpoint arrest. CAPN7 proteolytic activity is also required for abscission and checkpoint maintenance. Hence, IST1 recruits CAPN7 to midbodies, where its proteolytic activity is required to regulate and complete abscission.https://elifesciences.org/articles/84515cytokinesisESCRT-IIIabscissionNoCut Checkpoint |
| spellingShingle | Elliott L Paine Jack J Skalicky Frank G Whitby Douglas R Mackay Katharine S Ullman Christopher P Hill Wesley I Sundquist The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission eLife cytokinesis ESCRT-III abscission NoCut Checkpoint |
| title | The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission |
| title_full | The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission |
| title_fullStr | The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission |
| title_full_unstemmed | The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission |
| title_short | The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission |
| title_sort | calpain 7 protease functions together with the escrt iii protein ist1 within the midbody to regulate the timing and completion of abscission |
| topic | cytokinesis ESCRT-III abscission NoCut Checkpoint |
| url | https://elifesciences.org/articles/84515 |
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