Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice
It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57B...
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2023-10-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9843 |
| _version_ | 1797261352199258112 |
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| author | Nejc Umek Luka Pušnik Chiedozie Kenneth Ugwoke Žiga Šink Simon Horvat Jiří Janáček Erika Cvetko |
| author_facet | Nejc Umek Luka Pušnik Chiedozie Kenneth Ugwoke Žiga Šink Simon Horvat Jiří Janáček Erika Cvetko |
| author_sort | Nejc Umek |
| collection | DOAJ |
| description |
It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM.
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| first_indexed | 2024-04-24T23:39:51Z |
| format | Article |
| id | doaj.art-2fc47ff125a04f5cb0116b639b4c7872 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:39:51Z |
| publishDate | 2023-10-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-2fc47ff125a04f5cb0116b639b4c78722024-03-15T13:22:21ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2023-10-0110.17305/bb.2023.9843Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female miceNejc Umek0https://orcid.org/0000-0001-5831-2216Luka Pušnik1https://orcid.org/0000-0003-3418-0348Chiedozie Kenneth Ugwoke2Žiga Šink3https://orcid.org/0009-0007-2454-6592Simon Horvat4Jiří Janáček5Erika Cvetko6Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaLaboratory of Biomathematics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech RepublicInstitute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM. https://www.bjbms.org/ojs/index.php/bjbms/article/view/9843Type 1 diabetes mellitus (T1DM)skeletal musclemyosin heavy chain (MyHC) isoformscapillary network analysismuscle fiber compositionthree-dimensional (3D) image analysis |
| spellingShingle | Nejc Umek Luka Pušnik Chiedozie Kenneth Ugwoke Žiga Šink Simon Horvat Jiří Janáček Erika Cvetko Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice Biomolecules & Biomedicine Type 1 diabetes mellitus (T1DM) skeletal muscle myosin heavy chain (MyHC) isoforms capillary network analysis muscle fiber composition three-dimensional (3D) image analysis |
| title | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
| title_full | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
| title_fullStr | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
| title_full_unstemmed | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
| title_short | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
| title_sort | skeletal muscle myosin heavy chain expression and 3d capillary network changes in streptozotocin induced diabetic female mice |
| topic | Type 1 diabetes mellitus (T1DM) skeletal muscle myosin heavy chain (MyHC) isoforms capillary network analysis muscle fiber composition three-dimensional (3D) image analysis |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9843 |
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