<italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis
Objectives:To describe the clinical features and microstructural characteristics assessed by <i>in vivo</i> confocal microscopy (IVCM) in patients with adenoviral epidemic keratoconjunctivitis (EKC).Materials and Methods:The study included 20 eyes of 12 patients who presented to the Koca...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2018-12-01
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| Series: | Türk Oftalmoloji Dergisi |
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http://www.oftalmoloji.org/archives/archive-detail/article-preview/in-vivo-i-confocal-microscopy-analysis-of-the-corn/21057
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| _version_ | 1828015705783533568 |
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| author | Sevgi Subaşı Nurşen Yüksel Müge Toprak Büşra Yılmaz Tuğan |
| author_facet | Sevgi Subaşı Nurşen Yüksel Müge Toprak Büşra Yılmaz Tuğan |
| author_sort | Sevgi Subaşı |
| collection | DOAJ |
| description | Objectives:To describe the clinical features and microstructural characteristics assessed by <i>in vivo</i> confocal microscopy (IVCM) in patients with adenoviral epidemic keratoconjunctivitis (EKC).Materials and Methods:The study included 20 eyes of 12 patients who presented to the Kocaeli University Medical Faculty, Department of Ophthalmology with complaints of watering, crusting, and stinging, were clinically diagnosed EKC, and were examined by slit-lamp biomicroscopy and IVCM during the prodromal phase and the punctate keratitis, deep epithelial keratitis, and subepithelial infiltration stages of EKC.Results:While biomicroscopic examination findings were normal during the prodromal period of EKC, IVCM showed an increase in Langerhans cell numbers in the subbasal plexus. After onset of clinical EKC, the punctate epithelial keratitis stage was characterized by findings of hyperreflective cell clusters in the basal epithelium layer, increased accumulation of Langerhans cells in Bowman’s layer, and hyperreflectivity in the anterior stromal layers. In the deep epithelial keratitis stage, the basal epithelial cells displayed peripheral hyperreflectivity and the hyperreflectivity of the anterior stromal surface increased and became more rounded. In the subepithelial keratitis stage, these findings persisted in addition to increased anterior stromal surface hyperreflectivity and focal round plaques.Conclusion:This study shows that the inflammatory process in the cornea starts in the prodromal period of EKC. Massive inflammation of the epithelium and stroma was observed in the active phase and focal changes were observed on the anterior stromal surface starting in the subepithelial infiltration period. |
| first_indexed | 2024-04-10T10:21:59Z |
| format | Article |
| id | doaj.art-2fca19af9f2c498db4861f0e250695c8 |
| institution | Directory Open Access Journal |
| issn | 1300-0659 2147-2661 |
| language | English |
| last_indexed | 2024-04-10T10:21:59Z |
| publishDate | 2018-12-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türk Oftalmoloji Dergisi |
| spelling | doaj.art-2fca19af9f2c498db4861f0e250695c82023-02-15T16:21:32ZengGalenos YayineviTürk Oftalmoloji Dergisi1300-06592147-26612018-12-0148627628010.4274/tjo.5925113049054<italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic KeratoconjunctivitisSevgi Subaşı0Nurşen Yüksel1Müge Toprak2Büşra Yılmaz Tuğan3 Körfez Devlet Hastanesi, Göz Hastalıkları Kliniği, Kocaeli, Türkiye Kocaeli Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Kocaeli, Türkiye Gebze Fatih Devlet Hastanesi, Göz Hastalıkları Kliniği, Kocaeli, Türkiye Ağrı Patnos Devlet Hastanesi, Göz Hastalıkları Kliniği, Ağrı, Türkiye Objectives:To describe the clinical features and microstructural characteristics assessed by <i>in vivo</i> confocal microscopy (IVCM) in patients with adenoviral epidemic keratoconjunctivitis (EKC).Materials and Methods:The study included 20 eyes of 12 patients who presented to the Kocaeli University Medical Faculty, Department of Ophthalmology with complaints of watering, crusting, and stinging, were clinically diagnosed EKC, and were examined by slit-lamp biomicroscopy and IVCM during the prodromal phase and the punctate keratitis, deep epithelial keratitis, and subepithelial infiltration stages of EKC.Results:While biomicroscopic examination findings were normal during the prodromal period of EKC, IVCM showed an increase in Langerhans cell numbers in the subbasal plexus. After onset of clinical EKC, the punctate epithelial keratitis stage was characterized by findings of hyperreflective cell clusters in the basal epithelium layer, increased accumulation of Langerhans cells in Bowman’s layer, and hyperreflectivity in the anterior stromal layers. In the deep epithelial keratitis stage, the basal epithelial cells displayed peripheral hyperreflectivity and the hyperreflectivity of the anterior stromal surface increased and became more rounded. In the subepithelial keratitis stage, these findings persisted in addition to increased anterior stromal surface hyperreflectivity and focal round plaques.Conclusion:This study shows that the inflammatory process in the cornea starts in the prodromal period of EKC. Massive inflammation of the epithelium and stroma was observed in the active phase and focal changes were observed on the anterior stromal surface starting in the subepithelial infiltration period. http://www.oftalmoloji.org/archives/archive-detail/article-preview/in-vivo-i-confocal-microscopy-analysis-of-the-corn/21057 Adenovirusepidemic keratoconjunctivitisepithelial keratitisconfocal microscopysubepithelial infiltrates |
| spellingShingle | Sevgi Subaşı Nurşen Yüksel Müge Toprak Büşra Yılmaz Tuğan <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis Türk Oftalmoloji Dergisi Adenovirus epidemic keratoconjunctivitis epithelial keratitis confocal microscopy subepithelial infiltrates |
| title | <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis |
| title_full | <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis |
| title_fullStr | <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis |
| title_full_unstemmed | <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis |
| title_short | <italic>In Vivo</italic> Confocal Microscopy Analysis of the Corneal Layers in Adenoviral Epidemic Keratoconjunctivitis |
| title_sort | italic in vivo italic confocal microscopy analysis of the corneal layers in adenoviral epidemic keratoconjunctivitis |
| topic | Adenovirus epidemic keratoconjunctivitis epithelial keratitis confocal microscopy subepithelial infiltrates |
| url |
http://www.oftalmoloji.org/archives/archive-detail/article-preview/in-vivo-i-confocal-microscopy-analysis-of-the-corn/21057
|
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